Abstract
A correlative light- and electron microscopical study has yielded both a description of chromosome behaviour during early meiotic prophase in mouse spermatocytes, and an accurate timing of the zygotene and early pachytene stage. The light microscopic part of the study consists of an analysis of orcein stained or C-banded, air-dried preparations, and series of separately squashed sections of seminiferous tubules. The mice used for air-dried preparations were treated with hydroxyurea and triaziquone, which reduces the spermatocyte population to a small, well-defined group of cells in meiotic interphase. The development of the restricted spermatocyte population is followed during four days. The electron microscopical study consists of an investigation of synaptonemal complexes in thin sections of seminiferous tubules in various stages of the epithelial cycle. It turned out that synaptic pairing starts about half a day after the end of the DNA replication phase. At most one and a half day later chromosome pairing is completed. In very early pachytene spermatocytes a sex vesicle is not visible. The orientation of the X and Y chromosome into a sex vesicle starts about one day after the beginning of the pachytene stage. The existence of a leptotene stage is discussed. The presence of a pre-leptotene contraction phase in male mice is excluded.
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Church, K.: Meiosis in the grasshopper. II. The preleptotene spiral stage during oogenesis and spermatogenesis in Melanoplus ferrumrubrum. Canad. J. Genet. Cytol. 14, 397–401 (1972)
Devictor-Vuillet, M., Luciani, J.M., Stahl, A.: Individualisation d'un stade préleptoténe de condensation chromosomique dans l'ovocyte de la lapine. C.R. Acad. Sci. Paris 276, 2453–2456 (1973)
Dietrich, A.J.J., Mulder, R.J.P.: A light microscopic study of the development and behaviour of the synaptonemal complex in spermatocytes of the mouse. Chromosoma (Berl.) 83, 409–418 (1981)
Driscoll, D.J., Palmer, C.G., Melman, A.: Nonhomologous associations of C-heterochromatin at human male meiotic prophase. Cytogenet. Cell Genet. 23, 23–32 (1979)
Grell, R.F., Oakberg, E.F., Generoso, E.E.: Synaptonemal complexes at premeiotic interphase in the mouse spermatocyte. Proc. nat. Acad. Sci. (Wash.) 77, 6720–6723 (1980)
John, B.: Myths and mechanisms in meiosis. Chromosoma (Berl.) 54, 295–325 (1976)
Hultén, M., Lindsten, J.: Cytogenetic aspects of human male meiosis. In: Advances in human genetics (H. Harris and K. Hirschhorn, eds.) vol. 4, pp. 327–387. New York: Plenum press 1973
Jong, J.H. de. Oud, J.L.: Location and behaviour of constitutive heterochromatin during meiotic prophase in Beta vulgaris L. Genetica (Den Haag) 51, 125–133 (1979)
McDermott, A.: Human male meiosis: chromosome behaviour at pre-meiotic and meiotic stages of spermatogenesis. Canad. J. Genet. Cytol. 13, 536–549 (1971)
Monesi, V.: Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testes, using tritiated thymidine. J. Cell Biol. 14, 1–18 (1962)
Oakberg, E.F.: A description of spermatogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Amer. J. Anat. 99, 391–409 (1956a)
Oakberg, E.F.: Duration of spermatogenesis in the mouse and timing of stages of the cycle of the seminiferous epithelium. Amer. J. Anat. 99, 507–516 (1956b)
Oud, J.L., Jong, J.H. de, Rooij, D.G. de: A sequential analysis of meiosis in the male mouse during a restricted spermatocyte population obtained by a hydroxyurea/triazquone treatment. Chromosoma (Berl.) 71, 237–248 (1979)
Oud, J.L., Reutlinger, A.H.H.: The behaviour of silver positive structures during meiotic prophase of male mice. Chromosoma (Berl.) 81, 569–578 (1981)
Rasmussen, S.W., Holm, P.B.: Human meiosis II. Chromosome pairing and recombination nodules in human spermatocytes. Carlsberg Res. Commun. 43, 275–327 (1978)
Rooij, D.G. de: Spermatogonial stem cell renewal in the mouse. I. Normal situation. Cell Tissue Kinet. 6, 281–287 (1973)
Stahl, A., Luciani, J.M., Devictor-Vuillet, M.: Étude chromosomique de la méiose. In: Les accidents chromosomiques de la reproduction (A. Boué and C. Thibault, eds.) pp. 197–218. Paris: Inserm 1973
Sumner, A.T.: A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75, 304–306 (1972)
Walters, M.S.: Premeiosis and meiosis in Lilium “Enchantment”. Chromosoma (Berl.) 80, 119–146 (1980)
Wilson, E.B.: The cell in development and heredity, 3rd edit. New York: Macmillan 1925
Winiwarter, H. von: Recherches sur l'ovogénèse et l'organogénèse de l'ovaire des mammifères (lapin et homme). Arch. Biol. (Liège, Paris) 17, 33–199 (1901)
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Oud, J.L., Reutlinger, A.H.H. Chromosome behaviour during early meiotic prophase of mouse primary spermatocytes. Chromosoma 83, 395–407 (1981). https://doi.org/10.1007/BF00327361
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DOI: https://doi.org/10.1007/BF00327361