Abstract
The seven chromosomes of barley (Hordeum vulgare) have been identified individually by their distinctive N-banding pattern. Furthermore all of the barley chromosome N-banding patterns were found to be recognizably different from those exhibited by wheat chromosomes, making it possible to identify individual barley chromosomes when present in a wheat background. N-banding has therefore been used to identify the individual barley chromosomes present in (a) reciprocal wheat-barley F1 hybrids, including some with abnormal chromosome constitution, and (b) a set of wheat-barley addition lines produced in this laboratory. The value of N-banding for detecting translocations between wheat and barley chromosomes and for isolating lines possessing a pair of barley chromosomes substituted for a particular pair of wheat chromosomes is also demonstrated.
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References
Burnham, C.R., Hagberg, A.: Cytogenetic notes on chromosomal interchanges in barley. Hereditas (Lund) 42, 467–482 (1956)
Darvey, N.L., Gustafson, J.P.: Identification of rye chromosomes in wheat-rye addition lines and triticale by heterochromatin bands. Crop. Sci. 15, 239–243 (1975)
Funaki, K., Matsui, S., Sasaki, M.: Location of nucleolar organizers in animal and plant chromosomes by means of an improved N-banding technique. Chromosoma (Berl.) 49, 357–370 (1975)
Gerlach, W.L.: N-banded karyotypes of wheat species. Chromosoma (Berl.) 62, 49–56 (1977)
Gill, B.S., Kimber, G.: Giemsa C-banding and the evolution of wheat. Proc. nat. Acad. Sci. (Wash.) 71, 4086–4090 (1974)
Islam, A.K.M.R., Shepherd, K.W., Sparrow, D.H.B.: Addition of individual barley chromosomes to wheat. Proc. 3rd Int. Barley Genet. Symp. (Garching, BRD) 260–270 (1975)
Islam, A.K.M.R., Shepherd, K.W., Sparrow, D.H.B.: Production and characterization of wheatbarley addition lines. Proc. 5th Int. Wheat Genet. Symp. (New Delhi) 365–371 (1978)
Künzel, G.: Indications for a necessary revision of the barley karyogramme by use of translocations. Proc. 3rd Int. Barley Genet. Symp. (Garching, BRD) 275–281 (1975)
Linde-Laursen, I.: Giemsa C-banding of the chromosomes of ‘Emir’ barley. Hereditas (Lund) 81, 285–289 (1975)
Linde-Laursen, I.: Giemsa C-banding of barley chromosomes I. Banding pattern polymorphism. Hereditas (Lund) 88, 55–64 (1978a)
Linde-Laursen, I.: Giemsa C-banding of barley chromosomes II. Banding patterns of trisomies and telotrisomics. Hereditas (Lund) 89, 37–41 (1978b)
Noda, K., Kasha, K.J.: Barley chromosome identification with the C-banding Giemsa stain technique. Barley Genet. Newsl. 6, 47–50 (1976)
Noda, K., Kasha, K.J.: A proposed barley karyotype revision based on C-band chromosome identification. Crop Sci. 18, 925–930 (1978)
Ramage, R.T., Burnham, C.R., Hagberg, A.: A summary of translocation studies in barley. Crop Sci. 1, 277–279 (1961)
Tjio, J.H., Hagberg, A.: Cytological studies on some x-ray mutants of barley. Anal. Estac. Exp. Aula Dei 2, 149–167 (1951)
Tsuchiya, T.: Cytogenetic studies of trisomies in barley. Jap. J. Bot. 17, 177–213 (1960)
Tsuchiya, T.: Studies on the trisomies in barley. II. Cytological identification of the extra chromosomes in crosses with Burnham's translocation testers. Jap. J. Genet. 36, 444–451 (1961)
Tuleen, N.A.: Karyotype analysis of multiple translocation stocks of barley. Canad. J. Genet. Cytol. 15, 267–273 (1973)
Vosa, C.G.: Chromosome banding patterns in cultivated and wild barleys (Hordeum spp.) Heredity (Lond.) 37, 395–403 (1976)
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Islam, A.K.M.R. Identification of wheat-barley addition lines with N-banding of chromosomes. Chromosoma 76, 365–373 (1980). https://doi.org/10.1007/BF00327272
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DOI: https://doi.org/10.1007/BF00327272