Abstract
The Saccharomyces cerevisiae MRS6 gene belongs to the same gene family as that responsible for the mammalian Rab escort protein (REP) and the Rab GDP dissociation inhibitor protein (GDI). Both REP and GDI are regulators of the Ras-related small G-proteins Rab/YPT1 which are involved in intracellular vesicular trafficking in yeast and in mammals. Here were characterize an antiserum directed against Mrs6p and show that it specifically inhibits the geranylation of the YPT1 protein in an in vitro assay. These findings provide direct evidence for the role of Mrs6p as the REP component of the yeast Rab geranylgeranyl transferase enzyme.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Araki S, Kaibuchi K, Sasaki T, Hata Y, Takai Y (1991) Mol Cell Biol 11:1438–1447
Boguski MS, McCormick F (1993) Nature 366:643–654
Bourne HR, Sanders DA, McCormick F (1991) Nature 349:117–127
Bradford M (1976) Anal Biochem 72:248–254
Fujimura K, Tanaka K, Nakano A, Toh-e A (1994) J Biol Chem 269: 9205–9212
Jiang Y, Rossi G, Ferro-Novick S (1993) Nature 366:84–86
Laemmli UK (1970) Nature 227:680
Li R, Havel C, Watson JA, Murray AW (1993) Nature 366:82–84
Molenaar CMT, Prange R, Gallwitz D (1988) EMBO J 7:971–976
Ragnini A, Teply R, Waldherr M, Voskova A, Schweyen RJ (1994) Curr Genet (in press)
Schägger H, von Jagow G (1987) Anal Biochem 166:368–379
Seabra MC, Brown MS, Slaughter CA, Sudhof TC, Goldstein JL (1992) Cell 70:1049–1057
Seabra MC, Brown MS, Goldstein JL (1993) Science 259:377–381
Schmitt HD, Puzicha M, Gallwitz D (1988) Cell 53:635–647
Waldherr M, Ragnini A, Jank B, Teply R, Wiesenberger G, Schweyen RJ (1993a) Curr Genet 24:301–306
Waldherr M, Ragnini A, Schweyen RJ, Boguski MS (1993b) Nature Genet 3:193–194
Author information
Authors and Affiliations
Additional information
Communicated by F. K. Zimmermann
Rights and permissions
About this article
Cite this article
Benito-Moreno, R.M., Miaczynska, M., Bauer, B.E. et al. Mrs6p, the yeast homologue of the mammalian choroideraemia protein: immunological evidence for its function as the Ypt1p Rab escort protein. Curr Genet 27, 23–25 (1994). https://doi.org/10.1007/BF00326574
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00326574