Abstract
A new technique (BUdR treatment followed by acridine orange staining) allowing a differentiation of sister chromatids is described. A statistical analysis of 91 human karyotypes gives an estimate of the frequency of exchanges. The mean of sister chromatid exchanges is 27,3 and the minimum number is 11 per cell. — The frequency of these exchanges is proportional to the relative length of each chromosome, and the accumulation of several exchanges in some segments evokes the possibility of a “negative interference”. — The analysis of endomitoses treated with BUdR during at least two generations is not in disagreement with the model of semi-conservative replication of chromosomal DNA, but the modifications of the chromatids may result from a completely different process. — The frequency of endomitoses is increased by the treatment. These endomitoses allow a very precise analysis of the evolution of the sister chromatid exchanges, during two successive cellular generations.
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Travail de l'E.R.A. N∘ 47 du C.N.R.S. et C.E.A. Contrat N∘ 293.
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Dutrillaux, B., Fosse, A.M., Prieur, M. et al. Analyse des échanges de chromatides dans les cellules somatiques humaines. Chromosoma 48, 327–340 (1974). https://doi.org/10.1007/BF00326509
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DOI: https://doi.org/10.1007/BF00326509