Abstract
The B mating type of the basidiomycete fungus, Schizophyllum commune is determined by two, tightly linked, multi-specificity (also called multi-allelic) loci: Bα and Bβ. A plasmid library was used in DNA-mediated transformation to obtain transformants that displayed B-directed development. Plasmids that conferred Bα1 and Bβ1 mating-type specificities were rescued from the transformants. Fragments of DNA from each plasmid hybridized to genomic DNA from the strain used to make the plasmid library; however, they did not hybridize, or hybridized only weakly, to genomic DNA from strains with mating-type specificities different from Bα1 or Bβ1. The cloned fragments are presumed to correspond to active regions of each B mating-type locus.
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References
Asgeirsdottir SA, Schuren FHJ, Wessels JGH (1994) Assignment of genes to pulse-field separated chromosomes of Schizophyllum commune. Mycol Res 98:689–693
Clewell D (1972) Nature of Col E1 plasmid replication in Escherichia coli in the presence of chloramphenicol J Bacteriol 110:667–676
Frankel C, Ellingboe AH (1976) New mutations and a 7-chromosome linkage map of Schizophyllum commune. Genetics 85:417–425
Froeliger EH, Munoz-Rivas AM, Specht CA, Ullrich RC, Novotny CP (1987) The isolation of specific genes from the basidiomycete Schizophyllum commune. Curr Genet 12:547–554
Giasson L, Specht CA, Milgrim C, Novotny CP, Ullrich RC (1989) Cloning and comparison of Aα mating-type alleles of the Basidiomycete Schizophyllum commune. Mol Gen Genet 218:72–77
Gillissen B, Bergemann J, Sandmann C, Schoeer B, Bolker M (1992) A two-component regulatory system for self/non-self recognition in Ustilago maydis. Cell 68:647–657
Koltin, Y (1968) The genetic structure of the incompatibility factors of Schizophyllum commune: comparative studies of primary mutations in the B factor. Mol Gen Genet 102:196–203
Koltin Y, Raper JR, Simchen G (1967) Genetic structure of the incompatibility factors of Schizophyllum commune: the B factor. Proc Natl Acad Sci USA 57:55–66
Kues U, Richardson WVJ, Tymon AM, Mutasa ES, Gottgens B, Gaubatz S, Gregoriades A, Casselton LA (1992) The combination of dissimilar alleles of the Aα and Aβ gene complexes, whose proteins contain homeo-domain motifs, determines sexual development in the mushroom Coprinus cinereus. Genes Dev 6:568–577
Luo Y, Ullrich RC, Novotny CP (1994) Only one of the paired Schizophyllum commune Aα mating-type, putative homeobox genes encodes a homeodomain essential for Aα-regulated development. Mol Gen Genet 244:318–324
May G, Le Chevanton L, Pukkila PJ (1991) Molecular analysis of the Coprinus cinereus mating type A factor demonstrates an unexpectedly complex structure. Genetics 128:529–538
Munoz-Rivas AM, Specht CA, Novotny CP, Ullrich RC (1986) Isolation of the DNA sequence coding indole-3-glycerol phosphate synthetase and phosphoribosylanthranilate isomerase of Schizophyllum commune. Curr Genet 10:909–913
Mutasa ES, Tymon AM, Bottgens B, Mellon FM, Little PFR, Casselton LA (1990) Molecular organization of an A mating-type factor of the basidiomycete fungus Coprinus cinereus. Curr Genet 18:223–229
Parag Y (1962) Mutations in the B incompatibility factor of Schizophyllum commune. Proc Natl Acad Sci USA 48:743–750
Raper CA (1983) Controls for development and differentiation of the dikaryon in Basidiomycetes. In: Bennett JW, Ceigler A (eds) Secondary metabolism and differentiation in fungi. Marcel Dekker, New York, pp 195–238
Raper CA (1988) Schizophyllum commune, a model for genetic studies of the Basidiomycotina. Adv Plant Pathal 6:511–522
Raper JR, Hoffman RM (1974) Schizophyllum commune. In: King RC (ed) Handbook of genetics. Plenum, New York, pp 597–626
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Specht CA, Dirusso CC, Novotny CP, Ullrich RC (1982) A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem 119:158–163
Specht CA, Munoz-Rivas AM, Novotny CP, Ullrich RC (1991) Transformation of Schizophyllum commune: an analysis of specific properties. Exp Mycol 15:326–335
Specht CA, Stankis MM, Giasson L, Novotny CP, Ullrich RC (1992) Functional analysis of the homeodomain-related proteins of the Aα locus of Schizophyllum commune. Proc Natl Acad Sci USA 89:7174–7178
Specht CA, Stankis MM, Novotny CP, Ullrich RC (1994) Mapping the heterogeneous DNA region that determines the nine Aα mating-type specificites of Schizophyllum commune. Genetics 137:709–714
Stankis MM, Specht CA, Giasson L (1990) Sexual incompatibility in Schizophyllum commune: from classical genetics to a molecular view. Sem Dev Biol 1:195–206
Stankis MM, Specht CA, Yang H, Giasson L, Ullrich RC, Novotny CP (1992) The Aα mating locus of Schizophyllum commune encodes two dissimilar, multiallelic, homeodomain proteins. Proc Natl Acad Sci USA 89:7169–7173
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Communicated by G. Simchen
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Specht, C.A. Isolation of the Bα and Bβ mating-type loci of Schizophyllum commune . Curr Genet 28, 374–379 (1995). https://doi.org/10.1007/BF00326436
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DOI: https://doi.org/10.1007/BF00326436