Abstract
We have constructed an ordered-array genomic DNA library of the pathogenic dimorphic fungus Candida albicans which facilitates the rapid cloning of C. albicans genes by hybridisation. Using the Saccharomyces cerevisiae SEC53 gene encoding phosphomannomutase as a hybridisation probe we have cloned the C. albicans homologue, PMM1, and determined its sequence. This gene shows high similarity, both at the nucleotide (76.2%) and amino-acid (77.7%) level, to the S. cerevisiae SEC53 gene. We have used the C. albicans PMM1 gene, in single copy, to transform temperature-sensitive S. cerevisiae sec53-6 mutant cells, which are defective in PMM activity at 37°C, to growth at 37°C. The C. albicans PMM1 gene is thus the structural and functional equivalent of the SEC53 gene.
References
Bernstein M, Hoffmann W, Ammerer G, Schekman R (1985) J Cell Biol 101: 2374–2382
Clarke L, Carbon J (1976) Cell 9: 91–99
Gorman JA, Chan W, Gorman JW (1991) Genetics 129: 19–24
Ito H, Fukada Y, Murata K, Kimura A (1983) J Bacteriol 153: 163–168
Kelly R, Miller SM, Kurtz MB, Kirsch DR (1987) Mol Cell Biol 7: 199–207
Kepes F, Schekman R (1988) J Biol Chem 263: 9155–9161
Rose MD, Novick P, Thomas JH, Botstein D, Fink GR (1987) Gene 60: 237–243
Sambrook JF, Maniatis T, Fritsch EF (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sherman F, Fink GR, Hicks JB (1982) Methods in yeast genetics: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Smith DJ, Proudfoot A, Friedli L, Klig LS, Paravicini G, Payton MA (1992) Mol Cell Biol 12: 2924–2930
Tanner W, Lehle L (1987) Biochem Biophys Acta 906: 81–99
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Communicated by C. P. Hollenberg
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Smith, D.J., Cooper, M., DeTiani, M. et al. The Candida albicans PMM1 gene encoding phosphomannomutase complements a Saccharomyces cerevisiae sec 53-6 mutation. Curr Genet 22, 501–503 (1992). https://doi.org/10.1007/BF00326416
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DOI: https://doi.org/10.1007/BF00326416