Abstract
At a time in the life cycle when a large proportion of the oocytes of Acheta incorporate 3H-thymidine into an extrachromosomal DNA body, synthesis of a satellite or minor band DNA, the density of which is greater than main band DNA, is readily detected. Synthesis of the satellite DNA is not detectable in tissues, the cells of which do not have a DNA body, or in ovaries in which synthesis of extrachromosomal DNA by the oocytes is completed. The DNA body contains the amplified genes which code for ribosomal RNA. However, less than 1 percent of the satellite DNA, all of which appears to be amplified in the oocyte, is complementary to ribosomal 18S and 28S RNA. In situ hybridization demonstrates that non-ribosomal elements, like the ribosomal elements of the satellite DNA, are localized in the DNA body.
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Abbreviations
- rRNA:
-
ribosomal RNA, includes 18S and 28S RNA
- rDNA:
-
gene sequences complementary to rRNA
- cRNA:
-
complementary RNA synthesized in vitro
References
Allen, E. R., Cave, M. D.: Formation, transport, and storage of ribonucleic acid containing structures in oocytes of Acheta domesticus (Orthoptera). Z. Zellforsch. 92, 477–486 (1968).
Allen, E. R., Cave, M. D.: Nucleolar organization in oocytes of Gryllid crickets: Subfamilies Gryllinae and Nemobiinae. J. Morph. 137, 433–447 (1972).
Bayreuther, K.: Die Oogenese der Tipuliden. Chromosoma (Berl.) 7, 508–557 (1956).
Bayreuther, K.: Extrachromosomales DNS-haltiges Material in der Oogenese der Flöhe. Z. Naturforsch. 12b, 458–463 (1957).
Birnstiel, M., Speirs, J., Purdom, I., Jones, K.: Properties and composition of the isolated ribosomal satellite of Xenopus laevis. Nature (Lond.) 219, 454–463 (1968).
Bishop, J. O., Robertson, F. W., Burns, J. A., Melli, M.: Methods for the analysis of deoxyribonucleic acid—ribonucleic acid hybridization data. Biochem. J. 115, 361–370 (1969).
Brown, D. D., Dawid, I. B.: Specific gene amplification in oocytes. Science 160, 272–280 (1968).
Brown, D. D., Dawid, I. B.: Developmental Genetics. Ann. Rev. Genet. 3, 127–154 (1969).
Brown, D. D., Gurdon, J.: Absence of ribosomal RNA synthesis in an anucleolate mutant of Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 51, 139–146 (1964).
Brown, D. D., Littna, E.: RNA synthesis during the development of Xenopus laevis, the South African clawed toad. J. molec. Biol. 8, 669–687 (1964).
Brown, D. D., Weber, C. S.: Gene linkage by RNA-DNA hybridization II. Arrangement of the redundant gene sequence for 28S and 18S ribosomal RNA. J. molec.Biol. 34, 681–697 (1968).
Brown, D. D., Weber, G. C., Sinclair, J. H.: Ribosomal RNA and its genes during oogenesis and development. Carnegie Inst. Wash. Year Book 66, 580–589 (1967).
Burgess, P. R., Travers, A. A., Dunn, J. J., Bautz, E. K. F.: Factors stimulating transcription by RNA polymerase. Nature (Lond.) 221, 43–46 (1969).
Cave, M. D.: Localization of ribosomal DNA within oocytes of the house cricket, Acheta domesticus (Orthoptera: Gryllidae). J. Cell Biol. 55, 310–321 (1972).
Cave, M. D., Allen, E. R.: Synthesis of nucleic acids associated with a DNA containing body of Acheta. Exp. Cell Res. 58, 201–212 (1969a).
Cave, M. D., Allen, E. R.: Extrachromosomal DNA in early stages of oogenesis in Acheta domesticus. J. Cell Sci. 4, 593–609 (1969b).
Collier, J. R.: Number of ribosomal cistrons in the marine mud snail, Ilyanassa obsoleta. Exp. Cell Res. 69, 181–184 (1971).
Dawid, I., Brown, D.: The mitochondrial and ribosomal DNA components of oocytes of Urechis caupo. Develop. Biol. 22, 1–14 (1970).
Dawid, I. B., Brown, D. D., Reeder, R. H.: Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis. J. molec. Biol. 51, 341–360 (1970).
Durand, M. C.: L'acide désoxyribonucléique des gamètes des Gryllus domesticus. C. R. Acad. Sci. (Paris) 241, 1340–1343 (1955).
Gall, J. G.: Differential synthesis of the genes for ribosomal RNA during amphibian oogenesis. Proc. nat. Acad. Sci. (Wash.) 60, 553–560 (1968).
Gall, J. G., Macgregor, H. C., Kidston, M. E.: Gene amplification in the oocytes of Dytiscid water beetles. Chromosoma (Berl.) 26, 169–187 (1969).
Gall, J. G., Pardue, M. L.: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. nat. Acad. Sci. (Wash.) 63, 378–383 (1969).
Gillespie, D.: The formation and detection of DNA-RNA hybrids. In: Methods in enzymology, vol. XII B (L. Grossman and K. Moldave, eds.), p. 641–668. New York: Academic Press 1968.
Hansen-Delkeskamp, E.: Satelliten-desoxyribonucleinsäure in Gonaden und somatischen Gewebe von Acheta domestica. L. Z. Naturforsch. 246, 1331–1335 (1969).
Heinonen, L., Halkka, O.: Early stages of oogenesis and metabolic DNA in oocytes of the house cricket, Acheta domesticus (L.) Ann. Med. exp. Fenn. 45, 101–109 (1967).
Ifft, J. B., Voet, D. H., Vinograd, J.: Determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. J. phys. Chem. 65, 1138–1145 (1961).
Kunz, W.: Lampbürstenchromosomen und multiple Nukleolen bei Orthopteren. Chromosoma (Berl.) 21, 446–462 (1967).
Kunz, W.: Die Entstehung multipler Oocytenukleolen aus akzessorischen DNA Körpern bei Gryllus domesticus. Chromosoma (Berl.) 26, 41–75 (1969).
Lima-de-Faria, A., Birnstiel, M. L., Jaworska, H.: Amplification of ribosomal cistrons in heterochromatin of Acheta. Genetics 61, Suppl., 145–159 (1969).
Lima-de-Faria, A., Nilsson, B., Cave, D., Puga, A., Jaworska, H.: Tritium labeling and cytochemistry of extra DNA in Acheta. Chromosoma (Berl.) 25, 1–20 (1968).
Mandel, M., Schildkraut, C., Marmur, J.: Use of CsCl density gradient analysis for determining the guanine plus cytosine content of DNA. In: Methods of enzymology, vol. XII B (L. Grossman and K. Moldave, editors), p. 184–195. New York: Academic Press 1968.
Miller, O., Beatty, B.: Visualization of nucleolar genes. Science 164, 955–957 (1969).
Pardue, M. L., Gerbi, S., Eckhardt, R., Gall, J.: Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma (Berl.) 29, 268–290 (1970).
Perkowska, E., Macgregor, H. C., Birnstiel, M. L.: Gene amplification in the oocyte nucleus of mutant and wild-type Xenopus laevis. Nature (Lond.) 217, 649–650 (1968).
Reeder, R. H., Brown, D. D.: Transcription of the ribosomal genes of an amphibian by the RNA polymerase of a bacterium. J. molec. Biol. 51, 361–377 (1970).
Ritossa, F. M., Atwood, K. C., Lindsley, D. L., Spiegelman, S.: On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA. Nat. Cancer Inst. Monogr. 23, 449–472 (1966).
Urbani, E.: Cytochemical and ultrastructural studies of oogenesis in the Dytiscidae. Monit. zool. ital. (N. S.) 3, 55–87 (1969).
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Cave, M.D. Synthesis and characterization of amplified DNA in oocytes of the house cricket, Acheta domesticus (Orthoptera: Gryllidae). Chromosoma 42, 1–22 (1973). https://doi.org/10.1007/BF00326327
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DOI: https://doi.org/10.1007/BF00326327