Abstract
Longitudinal thin sections of preselected spermatocytes were studied with the electron microscope. The kinetochores of autosomes and sex chromosomes show a characteristic change of their form during the meiotic divisions. Just after nuclear membrane breakdown the kinetochore profiles have the form of circles, in early prometaphase they have flame shape, and in metaphase appear as straight zones. As early as prometaphase I two sister kinetochores are discernible in each kinetochore region of a dyad. In prometaphase the sister kinetochores of the sex chromosomes are connected with each other through condensation zones which are continuous with both kinetochores. A double line structure is often seen in kinetochores and condensation zones. The morphological change of kinetochores can be asynchronous, as is especially conspicuous in the sex chromosomes. —The mitotic apparatuses of Pales behave in hexylenglycol medium like mitotic apparatuses of marine eggs. Crystalloids (Fuge, 1970) and microfilament bundles (Bajer and Molè-Bajer, 1969) occur in mitotic apparatuses in early and middle prometaphase.
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Literatur
Bajer, A.: Subchromatid structure of chromosomes in the living state. Chromosoma (Berl.) 17, 291–302 (1965).
Bajer, A., Molè-Bajer, J.: Formation of spindle fibers, kinetochore orientation, and behavior of the nuclear envelope during mitosis in endosperm. Chromosoma (Berl.) 27, 448–484 (1969).
Bajer, A., Molè-Bajer, J.: Architecture and function of the mitotic spindle. Advanc. Cell molec. Biol. 1, 213–266 (1971).
Bauer, H., Dietz, R., Röbbelen, C.: Die Spermatocytenteilungen der Tipuliden. III. Mitteilung. Das Bewegungsverhalten der Chromosomen in Translokationsheterozygoten von Tipula oleracea. Chromosoma (Berl.) 12, 116–189 (1961).
Behnke, O., Forer, A.: Some aspects of microtubules in spermatocyte meiosis in a crane fly (Nephrotoma suturalis Loew): Intranuclear and intrachromosomal microtubules. C. R. Lab. Carlsberg 35, 437–455 (1966).
Braselton, J. P., Bowen, C. C.: The ultrastructure of the kinetochores of Lilium longiflorum during the first meiotic division. Caryologia (Firenze) 24, 49–58 (1971).
Brinkley, B. R., Stubblefield, E.: The fine structure of the kinetochore of a mammalian cell in vitro. Chromosoma (Berl.) 19, 28–43 (1966).
Brinkley, B. R., Stubblefield, E.: Ultrastructure and interaction of the kinetochore and centriole in mitosis and meiosis. Advanc. Cell Biol. 1, 119–185 (1970).
Buck, R. C.: Mitosis and meiosis in Rhodnius prolixus: The fine structure of the spindle and diffuse kinetochore. J. Ultrastruct. Res. 18, 489–501 (1967).
Cohen, W. D., Gottlieb, T.: C-microtubules in isolated mitotic spindles. J. Cell Sci. 9, 603–619 (1971).
Comings, D. E., Okada, T. A.: Fine structure of Kinetochore in Indian Muntjac. Exp. Cell Res. 67, 97–110 (1971).
Dietz, R.: Multiple Geschlechtschromosomen bei den cypriden Ostracoden, ihre Evolution und ihr Teilungsverhalten. Chromosoma (Berl.) 9, 359–440 (1958).
Dietz, R.: Polarisationsmikroskopische Befunde zur chromosomeninduzierten Spindelbildung bei der Tipulide Pales crocata (Nematocera). Zool. Anz., Suppl. 26, 131–138 (1963).
Dietz, R.: Bau und Funktion des Spindelapparats. Naturwissenschaften 56, 237–248 (1969).
Forer, A.: Chromosome movements during cell-divison. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), p. 553–601. Amsterdam and London: North-Holland Publ. Co. 1969.
Frigerio, N. A., Shaw, M. J.: A simple method for determination of glutaraldehyde. J. Histochem. Cytochem. 17, 176–181 (1969).
Fuge, H.: A crystalloid component in the meiotic spindle. Exp. Cell Res. 60, 309–313 (1970).
Fuge, H.: Spindelbau, Mikrotubuliverteilung und Chromosomenstruktur während der I. meiotischen Teilung der Spermatocyten von Pales ferruginea. Z. Zellforsch. 120, 579–599 (1971).
Fuge, H., Müller, W.: Mikrotubuli-Kontakt an Anaphasechromosomen in der I. meiotischen Teilung. Exp. Cell Res. 71, 241–245.
Goldman, R. D., Rebhun, L. I.: The structure and some properties of the isolated mitotic apparatus. J. Cell Sci. 4, 179–209 (1969).
Jokelainen, P. T.: The ultrastructure and spatial organization of the metaphase kinetochore in mitotic rat cells. J. Ultrastruct. Res. 19, 19–44 (1967).
Jokelainen, P. T.: The effect of colchicine on the kinetochores and mitotic apparatus in the rat. J. Cell Biol. 39, 68a (1968).
Journey, L. J., Whaley, A.: Kinetochore ultrastructure in vincristine-treated mammalian cells. J. Cell Sci. 7, 49–54 (1970).
Luykx, P.: The structure of the kinetochore in meiosis and mitosis in Urechis eggs. Exp. Cell Res. 39, 643–657 (1965a).
Luykx, P.: Kinetochore-to-pole connections during prometaphase of the meiotic divisions in Urechis eggs. Exp. Cell Res. 39, 658–688 (1965b).
Luykx, P.: Cellular mechanisms of chromosome distribution. Int. Rev. Cytol., Suppl. 2, 1–173 (1970).
McCully, E. K., Robinow, C. F.: Mitosis in the fission yeast Schizosaccharomyces pombe: A comparative study with light and electron microscopy. J. Cell Sci. 9, 475–507 (1971).
Molè-Bajer, J.: Fine structural studies of apolar mitosis. Chromosoma (Berl.) 26, 427–448 (1969).
Moses, M. J.: Synaptinemal complex. Ann. Rev. Genet. 2, 363–412 (1968).
Müller, W.: Interferenzmikroskopische Untersuchungen der Trochenmassenkonzentration in isolierten Mitoseapparaten und lebenden Spermatocyten von Pales ferruginea (Nematocera). Chromosoma (Berl.) 30, 305–316 (1970).
Nicklas, R. B.: Mitosis. Advanc. Cell Biol. 2 (im Druck, 1972).
Nicklas, R. B., Staehly, C. A.: Chromosome micromanipulation. I. the mechanics of chromosome attachment to the spindle. Chromosoma (Berl.) 21, 1–16 (1967).
Schrader, F.: The structure of the kinetochore at meiosis. Chromosoma (Berl.) 1, 230–237 (1939).
Schrader, F.: Mitosis, 2nd ed. 1953. New York: Columbia University Press.
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Müller, W. Elektronenmikroskopische Untersuchungen zum Formwechsel der Kinetochoren während der Spermatocytenteilungen von Pales ferruginea (Nematocera) . Chromosoma 38, 139–172 (1972). https://doi.org/10.1007/BF00326191
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DOI: https://doi.org/10.1007/BF00326191