Abstract
Patterns of puffing activity during the third larval instar and the prepupal period of two different strains of D. melanogaster (Oregon and vg6) are compared. The variation in puffing activity observed is both quantitative (involving the mean size or timing of activity of individual puffs) and qualitative. The pattern of activity of 64% of the puffs is the same in the two strains, 12% show strain differences in puff size and 19% in the time of their activity. One puff (64C) is active only in one of the strains (vg6). In genetic experiments this puff segregates normally and the puff locus has been mapped genetically to a site coincident with, or at least very close to, the cytogenetic position of the puff. In heterozygotes the puff is homozygous only when the maternal and paternal homologues are synapsed. When the homologues are asynapsed only the homologue from the vg6 parent is puffed at 64C. With the exeption of some strains closely related to vg6 no other strain of D. melanogaster has been found to possess puffing activity at 64C. In vg6/In(3LR)C165 heterozygotes 64C forms a heterozygous puff even when the homologues are synapsed. In the discussion consideration is given to the various factors that control puff size.
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References
Ashburner, M.: Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila, melanogaster. Chromosoma (Berl.) 21, 398–428 (1967a).
Ashburner, M.: Gene activity dependent on chromosome synapsis in the polytene chromosomes of Drosophila melanogaster. Nature (Lond.) 214, 1159–1160 (1967b).
- Studies of puffing in the salivary gland chromosomes of Drosophila. Ph. D. Thesis, University of Cambridge (1968).
— Patterns of puffing activity in the salivary gland chromosomes of Drosophila. II. A comparison of the autosomal puffing patterns of the sibling species Drosophila melanogaster and D. simulans. Chromosoma (Berl.) 27, 47–63 (1969a).
— The genetic control of puffing in polytene chromosomes. In: Chromosomes today, vol. 2 (C.D. Darlington and K. R. Lewis eds.). Edinburgh: Oliver & Boyd 1969b.
Baker, R. F., and C. Yanofsky: The periodicity of RNA polymerase initiations: A new regulatory feature of transcription. Proc. nat. Acad. Sci. (Wash.) 60, 313–320 (1968).
Becker, H. J.: Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. I. Beobachtungen zum Verhalten des Puffmusters im Normalstamm und bei zwei Mutanten, giant und lethal-giant-larvae. Chromosoma (Berl.) 10, 654–678 (1959).
Beermann, W.: Nuclear differentiation and functional morphology of the chromosomes. Cold Spr. Harb. Symp. quant. Biol. 21, 217–232 (1956).
— Riesenchromosomen. Protoplasmatologia, Bd. VI D; p. 1–161. Wien: Springer 1962.
— Operative Gliederung der Chromosomen. Naturwissenschaften 52, 365–375 (1965).
— Differentiation at the level of the chromosomes. In: Cell differentiation and morphogenesis. Amsterdam: North-Holland Publ. Co. 1966.
Berendes, H. D.: Salivary gland function and chromosomal puffing patterns in Drosophila hydei. Chromosoma (Berl.) 17, 35–77 (1965).
Bridges, C. B., and K. S. Brehme: The mutants of Drosophila melanogaster. Carnegie Inst. Washington Publ. 552, p. 1–257 (1944).
—, and T. H. Morgan: The third chromosome group of mutant characters of Drosophila melanogaster. Carnegie Inst. Washington Publ. 327, p. 1–251 (1923).
Britten, R. J., and D. E. Kohne: Repeated sequences in DNA. Science 161, 529–540 (1968).
Callan, H. G.: The organisation of genetic units in chromosomes. J. Cell Sci. 2, 1–7 (1967).
Clever, U.: Actinomycin and puromycin: Effects on sequential gene activation by ecdysone. Science 146, 794–795 (1964).
— Control of chromosomal puffing. In: Control of nuclear activity (L. Goldstein, ed). Englewood Cliffs (N.J.): Prentice-Hall, Inc. 1967.
Henderson, S. A.: Chromosome pairing, chiasmata and crossing over. Handbook of molecular cytology (A. Lima-de-Faria, ed.). Amsterdam: North Holland Publishing Comp. 1969.
Imamoto, F.: On the initiation of transcription of the tryptophan operon in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 60, 305–312 (1968).
Keyl, H. G.: Duplikationen von Untereinheiten der chromosomalen DNS während der Evolution von Chironomus thummi. Chromosoma (Berl.) 17, 139–180 (1965a).
— A demonstrable local and geometric increase in the chromosal DNA of Chironomus. Experientia (Basel) 21, 191–193 (1965b).
— Increase of DNA in chromosomes. In: Chromosomes today, vol. 1 (C. D. Darlington and K. R. Lewis, eds.). Edinburgh: Oliver & Boyd 1966.
Kiknadze, I. I.: The structural and cytochemioal characteristics of chromosome puffs. In: Genetic variations in somatic cells (J. Klein, M. Vojtiskova, and V. Zeleny, eds.). Praha: Academia-Prague 1967.
Lindsley, D., and E. H. Grell: Genetic variations of Drosophila melanogaster. Carnegie Inst. Washington Publ. 627, p. 1–472 (1968).
Lytchev, V. A.: A study of the activity of chromosomes (after) continuous inbreeding in Drosophila melanogaster. [In Russian with English summary.] Tsitologia (U.S.S.R.) 1965, 325–2333.
-, and Zh. A. Medvedev: Some methodical aspects of the study of puffs in salivary gland chromosomes of Diptera in the experimental investigations of the influence of different factors on the chromosomes. [In Russian with English summary.] Genetika (U.S.S.R.) 1967 (8), 53–59.
Mechelke, P.: Das Wandern des Aktivitätmaximums im BR 4-Locus von Acricotopus lucidus als Modell für die Wirkungsweise eines komplexen Locus. Naturwissenschaften 48, 29 (1961).
Panitz, R.: Heterozygote Punktionsstrukturen in den Riesenchromosomen von Acricotopus lueidus. Puffs als Orte unilokaler Strukturmutationen. Chromosoma (Berl.) 17, 199–218 (1965).
Pavan, C., and A. L. P. Perondini: Heterozygous puffs and bands in Sciara ocellaris Compstock (1882). Exp. Cell Res. 48, 202–205 (1967).
Pelling, C.: Ribonukleinsäure-Synthese der Riesenchromosomen. Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma (Berl.) 15, 71–122 (1964).
Ritossa, F., and S. Spiegelman: Localisation of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965).
Roberts, P. A.: A positive correlation between crossing over within heterozygous pericentric inversions and reduced egg hatch of Drosophila females. Genetics 56, 179–187 (1967).
Spickett, S. G.: Genetic and developmental studies of a quantitative character. Nature (Lond.) 199, 870–873 (1963).
—, and J. M. Thoday: Regular responses to selection III. Interaction between located polygenes. Genet. Res. 7, 96–121 (1966).
Stevens, B. J., and H. Swift: RNA transport from nucleus to cytoplasm in Chironomus salivary glands. J. Cell Biol. 31, 55–77 (1966).
Thoday, J. M., and T. B. Boam: Regular responses to selection I. Description of responses. Genet. Res. 2, 161–176 (1961).
— J. B. Gibson, and S. G. Spickett: Regular responses to selection II. Recombination and accelerated response. Genet. Res. 5, 1–19 (1964).
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Ashburner, M. Patterns of puffing activity in the salivary gland chromosomes of Drosophila . Chromosoma 27, 156–177 (1969). https://doi.org/10.1007/BF00326142
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DOI: https://doi.org/10.1007/BF00326142