Summary
Mitosis in endosperm of Haemanthus katherinae was studied in vitro by the use of 16 mm time-lapse microcinematography. In several cells, chromosomal fibres were seen in phase contrast microscope. The fibres are more convergent in late metaphase and anaphase than in prometaphase. There exist two distinct fibre attachments at each daughter kinetochore, i.e. there are four in metaphase chromosome.
Details of the division of a nearly cytoplasm-free mitotic apparatus are also reported as well as some data concerning the elongation of the spindle and activity of the phragmoplast.
Similar content being viewed by others
References
Bajer, A.: Cine micrographic studies on chromosome movements in β-irradiated cells. Chromosoma (Berl.) 9, 319–331 (1958a); - Cine-micrographic studies on mitosis in endosperm. IV. Exp. Cell Res. 14, 245–256 (1958b); - A note on the behaviour of the spindle fibres at mitosis. Chromosoma (Berl.) 12, 64–71 (1961); - Cine-micrographic analysis of cell plate formation in endosperm. Exp. Cell Res. 37, 376–398 (1965a); - Chromosome distribution and separation. Symp. XI. Int. Congr. of Genetics (in the press) (1965b).
-, and J. Molé-Bajer: Mitosis and mitotic factors. 16 mm film 1963.
Cleveland, L. R.: The whole life cycle of chromosomes and their coiling systems. Trans. Amer. phil Soc. 39, 1–100 (1949).
Cooper, K. W.: Visibility of primary spindle fibres and the course of mitosis in the living blastomeres of the mite Pediculopsis graminum. Proc. nat. Acad. Sci. (Wash.) 27, 480–483 (1941).
Hughes, A. F., and M. M. Swann: Anaphase movement in the living cell. J. exp. Biol. 25, 45–70 (1948).
Inoué, S.: Polarization optical studies of the mitotic spindle. I. Chromosoma (Berl.) 5, 487–500 (1953).
—, and Bajer, A.: Birefringence in endosperm mitosis. Chromosoma (Berl.) 12, 48–63 (1961).
Lima-de-Faria, A.: Recent advances in the study of the kinetochore. Int. Rev. Cytol. 7, 123–157 (1958);- Matrix and kinetochore in living material. Hereditas (Lund) 45, 463–465 (1959).
Mazia, D.: Mitosis and the physiology of cell division. In: The cell, vol. III, edts. J. Brachet and A. Mirsky. New York: Academic Press Inc. 1961.
Molé-Bajer, J.: Some problems of the dynamics of the mitotic spindle and phragmoplast (in Polish). Univ. Jagellonica Acta Sci. Litt. 44, 1–88 (1962).
—, and A. Bajer: Mitosis in endosperm: technique of studies in vitro. Cellule 63, 399–407 (1963).
Östergren, G.: Proximal heterochromatin, structure of centromere and the mechanism of its misdivision. Bot. Notiser 1947, 176–177 (1947);-Luzula and the mechanism of chromosome movements. Hereditas (Lund) 35, 445–468 (1949); - The mechanism of co-orientation in bivalents and multivalents. Hereditas (Lund) 37, 85–156 (1951).
Ris, H. H.: The anaphase movement of chromosomes in the spermatocytes of grasshopper. Biol. Bull. 96, 90–106 (1949).
Roth, L. E.,and E. W. Daniels: Electron microscopic studies of mitosis in amebae. J. Cell Biol. 12, 57–78 (1962).
Schmidt, W. J.: Doppelbrechung der Kernspindel und Zugfasertheorie der Chromosomenbewegung. Chromosoma (Berl.) 1, 253–264 (1939).
Schrader, F.: Mitosis, 2nd ed. New York: Columbia University Press 1953.
Swann, M. M.: Protoplasmic structure and mitosis. I. J. exp. Biol. 28, 417–433 (1951a); - Protoplasmic structure and mitosis. II. J. exp. Biol. 28, 434–444 (1951b).
Author information
Authors and Affiliations
Additional information
Dedicated to Professor H. Bauer on the occasion of his sixtieth birthday.
Rights and permissions
About this article
Cite this article
Bajer, A. Behaviour of chromosomal spindle fibres in living cells. Chromosoma 16, 381–390 (1965). https://doi.org/10.1007/BF00326001
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00326001