Summary
Total DNAs from twelve natural isolates of Escherichia coli from animals and humans were examined by hybridization with a probe for IS1. Considerable variation in copy number was found. In the case of two strains isolated from the same individual, one strain contained no copies of IS1 and the other, much greater than 30. Evidence was also obtained for the existence of IS1-like elements (iso-IS1s) of greater than 15% sequence divergence relative to the IS1 from antibiotic resistance plasmid R100.
References
Brenner DJ, Fanning GR, Miklos, GV, Steigerwalt, AG (1973) Polynucleotide sequence relatedness among Shigella species. Int J Sys Bacteriol 23:1–7
Clements MB, Syvanen M (1980) Isolation of a polA mutation that affects transposition of insertion sequences and transposons. Cold Spring Harbor Symp Quant Biol 45:201–204
Colwell RR, Johnson R, Wan L, Lovelace TE, Brenner DJ (1971) Numerical taxonomy and deoxyribonucleic acid reassociation in the taxonomy of some Gram-negative fermentative bacteria. Int J Sys Bacteriol 24:422–433
Harshman L, Riley M (1980) Conservation and variation of nucleotide sequences in Escherichia coli strains isolated from nature. J Bacteriol 144:560–568
Lederberg J (1951) Genetic studies with bacteria. In: Dunn LC (ed) Genetics in the 20th Century. Macmillian, New York, p 263–289
Milkman R (1973) Electrophoretic variation in Escherichia coli from natural sources. Science 182:1024–1026
Milkman R (1975) Alloenzyme variation in Escherichia coli of diverse natural origins. In: Market CL (ed) Isozymes, vol 4. Genetics and evolution. Academic Press, New York, p 273–285
Nevers P, Reif H-J, Saedler HC (1977) On mutations affecting IS1-mediated deletion formation in E. coli. In: Bukhari A, Shapiro J, Adhya SL (eds) DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory, New York, p 125–131
Nyman K, Nakamura K, Ohtsubo H, Ohtsubo E (1981) Distribution of the insertion element IS1 in gram-negative bacteria. Nature (London) 289:609–612
Ohtsubo H, Nyman K, Doroskiewicz W, Ohtsubo E (1981) Multiple copies of iso-insertion sequences of IS1 in Shigella dysenteriae chromosome. Nature (London) 292:640–643
Ohtsubo E, Nyman K, Nakamura K, Ohtsubo H (1981) Evolution of the insertion element IS1 that causes genetic engineering of bacterial genomes in vitro. In: Setlow JK, and Hollaender A (eds) Genetic engineering, vol 3. Plenum Publishing Corporation New York, p 33–55
Ohtsubo H, Ohtsubo E (1978) Nucleotide sequence of an insertion element IS1. Proc Natl Acad Sci USA 75:615–619
Selander RK, Levin BR (1980) Genetic diversity and structure in Escherichia coli populations. Science 210:545–547
Smirnov GB, Ilina TS, Romonova YM, Markov AP, Nechaeva EV (1980) Mutants of Escherichia coli affected in the processes of transposition and genomic rearrangments. Cold Spring Harbor Symp Quant Biol 45:193–200
Southern E (1975) Detection of, specific sequences among DNA fragments by gel electrophoresis. J Mol Biol 98:503–517
Sternglanz R, DiNardo S, Voekel KA, Nishumura Y, Hirota Y, Becherer K, Zumstein L, Wang JG (1981) Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition. Proc Natl Acad Sci USA 78:2747–2751
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Communicated by G. O'Donovan
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Nyman, K., Ohtsubo, H., Davison, D. et al. Distribution of insertion element IS1 in natural isolates of Escherichia coli . Molec. Gen. Genet. 189, 516–518 (1983). https://doi.org/10.1007/BF00325920
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DOI: https://doi.org/10.1007/BF00325920