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The use of genetic markers selected in vitro for the isolation and genetic verification of intraspecific somatic hybrids of Nicotiana tabacum L.

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Summary

A scheme employing genetic markers obtained by in vitro selection was developed for the stringent isolation of hybrid somatic cells of Nicotiana tabacum. Mesophyll protoplasts that carried two dominant alleles of nuclear genes conferring resistance to the herbicide picloram (pmR1) and the ability to utilize glycerol as the sole source of carbon (Gut) were fused with suspension-culture protoplasts that were marked with the dominant nuclear allele (HuR9) conferring resistance to hydroxyurea. Putative somatic hybrid cell lines were identified by selecting for the Gut and HuR9 markers, followed by an assay for the unselected marker PmR1. Plants regenerated from six of these cell lines were proved to be true somatic hybrids by demonstrating the segregation of each of the three parental markers in the progeny of crosses of those plants with normal seed-derived plants.

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Author notes

  1. Stephen V. Evola

    Present address: Biology Department, Brookhaven National Laboratory, 11973, Upton, NY, USA

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853, Ithaca, NY, USA

    Stephen V. Evola & Elizabeth D. Earle

  2. Experimental Station, E.I. du Pont deNemours and Co., Inc., 19898, Wilmington, DE, USA

    Roy S. Chaleff

Authors
  1. Stephen V. Evola
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  2. Elizabeth D. Earle
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  3. Roy S. Chaleff
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Communicated by G. Melchers

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Evola, S.V., Earle, E.D. & Chaleff, R.S. The use of genetic markers selected in vitro for the isolation and genetic verification of intraspecific somatic hybrids of Nicotiana tabacum L.. Molec. Gen. Genet. 189, 441–446 (1983). https://doi.org/10.1007/BF00325906

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  • DOI: https://doi.org/10.1007/BF00325906

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