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Regulation of the lactose operon in vitro

II. Repression of transcription of the lactose operon

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Summary

Using the system described in the preceding paper (Iida et al., 1970), we have shown that lac repressor fractions purified one hundred to several hundred-fold repress the transcription of the lac operon in vitro and that an inducer IPTG1 inhibits the repression. The amount of lac messenger RNA produced in the case of the most powerful repression is calculated to be 1/10 of that in the presence of the inducer. In contrast, when the template DNA carries an operator constitutive (o c) mutation, little or no repression was observed. An efficient repression requires besides a purified repressor fraction, the presence of a cellular ribosomal fraction and an adequate salt concentration.

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Author notes

  1. Yoshiko Iida

    Present address: Institute of Physiology, Czechoslovak Academy of Sciences, Budějovická, 1083, Prague 4, Czechoslovakia

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan

    Yasumi Ohshima & Tadao Horiuchi

  2. The Cancer Research Institute, Kanazawa University, Kanazawa, Japan

    Yoshiko Iida & Tadanori Kameyama

Authors
  1. Yasumi Ohshima
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  2. Tadao Horiuchi
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  3. Yoshiko Iida
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  4. Tadanori Kameyama
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Communicated by J. Tomizawa

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Ohshima, Y., Horiuchi, T., Iida, Y. et al. Regulation of the lactose operon in vitro . Molec. Gen. Genetics 106, 307–314 (1970). https://doi.org/10.1007/BF00324048

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  • DOI: https://doi.org/10.1007/BF00324048

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