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A cell line from trypsinized adult rabbit brain tissue

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Summary

Trypsinized cerebral hemispheres, cerebellum or pial material from young adult rabbits were cultivated through many passages and for more than 6 months. Longterm cultures of the various tissues were undistinguishable and ultimately demonstrated a clearcut shift to hyperploidy. However, primary cultures and those in the first few passages showed a number of morphologically different cell types. Some were clearly recognizable as fibrous astrocytes, while others exhibited many characteristics of protoplasmic astrocytes. With the aid of time-lapse cinematography individual elements were found to undergo morphological transformation, even to losing their characteristic features. A third type of cell, clearly distinguishable from the others by its epithelial arrangement, probably was of endothelial origin. Ultimately the cell line obtained from all three sources bore a closer resemblance to the endothelial than to the astrocytic elements.

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Authors and Affiliations

  1. Pasadena Foundation for Medical Research, Pasadena, Calif., USA

    Silvio S. Varon M. D. (Senior Researcher, Assistant at the Institute of Psychiatry), Charles W. Raiborn Jr., T. Seto & C. M. Pomerat

  2. Department of Biochemistry, City of Hope Medical Center, Duarte, California

    Silvio S. Varon M. D. (Senior Researcher, Assistant at the Institute of Psychiatry)

  3. University of Milano (Italy) Medical School, Italy

    Silvio S. Varon M. D. (Senior Researcher, Assistant at the Institute of Psychiatry)

Authors
  1. Silvio S. Varon M. D.
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  2. Charles W. Raiborn Jr.
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  3. T. Seto
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  4. C. M. Pomerat
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Additional information

This investigation was supported in part by PHS Research Grants B-3113 (C 1) and C-2655 (C 2) from the National Institute of Neurological Diseases and Blindness, Public Health Service, and in part by the National Association for Mental Health.

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Varon, S.S., Raiborn, C.W., Seto, T. et al. A cell line from trypsinized adult rabbit brain tissue. Z. Zellforsch. 59, 35–46 (1963). https://doi.org/10.1007/BF00321006

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  • DOI: https://doi.org/10.1007/BF00321006

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