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Influences of clotting factors (thrombin, factor XIII) and of fibronectin on the growth of tumor cells and leukemic cells in vitro

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Summary

Thrombin, factor XIII and fibronectin were incubated with cultures of mouse sarcoma cells, human cervix carcinoma cells (HeLa cells) and cells of an acute lymphoblastic leukemia. Thrombin induced a significant increase of 3H-thymidine uptake into cells with a 1,5- to 2-fold increase of cell count. The cells of an acute lymphoblastic leukemia showed a similar response to the influence of thrombin. Factor XIII in tumor cells merely induced an increase of 3H-thymidine uptake, the cell count remained constant. The cells of an acute lymphoblastic leukemia showed under the influence of factor XIII a significant increase of cell count and thymidine uptake. HeLa cell growth was optimal at low fibronectin concentrations. Fibronectin concentrations of 1 mg/ml to 3 mg/ml inhibited HeLa and mouse sarcoma cell growth.

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Authors and Affiliations

  1. I. Medizinische Klinik Kiel, Klinikum der Christian-Albrechts-Universität, Schittenhelmstraße 12, D-2300, Kiel, Federal Republic of Germany

    H. D. Bruhn & K. H. Zurborn

Authors
  1. H. D. Bruhn
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  2. K. H. Zurborn
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Lectures held at the conference on “Aktuelle Mitteilungen/Work in Progress” of the Annual Meeting of the Deutsche und Österreichische Gesellschaft für Hämatologie und Onkologie, Innsbruck, October 1982

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Bruhn, H.D., Zurborn, K.H. Influences of clotting factors (thrombin, factor XIII) and of fibronectin on the growth of tumor cells and leukemic cells in vitro. Blut 46, 85–88 (1983). https://doi.org/10.1007/BF00320663

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  • DOI: https://doi.org/10.1007/BF00320663

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