Summary
1. Mucosal transferrin was determined as transferrin-like immunoreactivity (TLIR) by means of a 2-site immunoradiometric assay (IRMA). Scraped-off mucosal tissue as well as isolated mucosal cells from the duodenum and jejunum of normal and iron-deficient rats before and after a washing procedure were examined. 2. In iron-deficient rats there was about twice as much TLIR in scraped-off mucosal tissue as in the untreated animals. In the duodenum and jejunum of normal and iron-deficient rats, TLIR contents of the isolated cells in the magnitude of 320–510 ng/mg dry weight were found. 3. Washing isolated cells three times in ice-cold Hank's solution resulted in a nearly tenfold decrease of TLIR content in all groups. In contrast the cells' RNA content remained unchanged.
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Osterloh, K., Schümann, K., Ehtechami, C. et al. Transferrin in isolated cells from rat duodenum and jejunum. Blut 51, 41–47 (1985). https://doi.org/10.1007/BF00320599
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DOI: https://doi.org/10.1007/BF00320599