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The effects of inhibitors of RNA and protein synthesis on cytological development during meiosis

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Summary

An examination of the correlation between RNA and protein synthesis occurring during meiosis and cytological development was made in Trillium erectum microsporocytes. Various reagents known to act at various steps of protein biosynthesis were administered to cultured buds at different developmental stages with more or less effect depending on the stage rather than the reagent.

Syntheses were found to be necessary for continued development of the microsporocytes during early prophase. Synthesis during meiotic prophase was also necessary for the maintainance of the condensed state of the late prophase chromosomes, the initial separation of the paired homologous chromosomes, and the orderly function of the spindle. Cytokinesis was readily disturbed at all treatment times. Pairing of homologous chromosomes was not affected and the prespecification of pairing is believed to occur at or near the time of DNA synthesis.

The results indicate that the syntheses occurring during meiosis can be correlated with cytological developmental processes.

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Authors and Affiliations

  1. Department of Botany, University of Illinois, USA

    C. Lindley Kemp

  2. Institut d'Histologie et d' Embryologie, École de Médecine, Université de Genève, Genève, Switzerland

    C. Lindley Kemp

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  1. C. Lindley Kemp
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Based on a thesis presented in partial fulfillment of the requirements for the Ph. D. degree at the University of Illinois, Department of Botany.

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Kemp, C.L. The effects of inhibitors of RNA and protein synthesis on cytological development during meiosis. Chromosoma 15, 652–665 (1964). https://doi.org/10.1007/BF00319997

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