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Afferent and efferent innervation of the rat esophagus

A tracing study with horseradish peroxidase and nuclear yellow

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Summary

The technique of retrograde labeling of nerve cells with HRP and nuclear yellow as well as transganglionic anterograde HRP-tracing of sensory projections into the CNS were used to establish the motor and sensory innervation pattern of two parts of the rat esophagus: the cervical and the abdominal segment. For comparison, also the innervation of the anterior wall of the stomach was studied.

Application of HRP to the cervical part of the esophagus resulted in bilateral labeling of neurons in the nucleus ambiguns exclusively, while application of the tracer to the abdominal part was followed by labeling of cells in both the nucleus ambiguus and the dorsal motor nucleus of the vagus. Application of tracer to the wall of the stomach caused labeling of cells in the dorsal motor nucleus of the vagus exclusively. Labeling appeared always bilaterally.

In all experiments there was a profuse labeling of primary afferent neurons with cell bodies in both nodose ganglia and endings in certain subnuclei of the solitary nucleus. Endings related to the cervical esophagus projected into the ventral subnuclei, projections from the abdominal esophagus were located in the ventral and medial subnuclei, those from the stomach in the medial subnucleus solely. The area postrema and the commissural nucleus received afferents from both organs, the esophagus and the stomach.

Double labeling experiments with HRP and nuclear yellow provided no signs of overlap of sensory innervation areas of the sites investigated in this study. Within the wall of the esophagus no labeled intramural cells nor nerve fibers were found in sections beyond the injection sites.

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References

  • Beckstead RM, Norgren R (1979) An autoradiographic examination of the central distribution of the trigeminal, facial, glossopharyngeal and vagal nerves in the monkey. J Comp Neurol 184:455–472

    Google Scholar 

  • Chernicky CL, Barnes KL, Ferrario CM, Conomy JP (1982) Brainstem distribution of neurons with efferent projections in the cervical vagus of the dog. Brain Res Bull 10:345–351

    Google Scholar 

  • Clerc N (1983) Histological characteristics of the lower oesophageal sphincter in the cat. Acta Anat 117:201–208

    Google Scholar 

  • Contreras RJ, Beckstead RM, Norgren R (1982) The central projections of the trigeminal, facial, glossopharyngeal and vagus nerves: an autoradiographic study in the rat. J Autonom Nerv Syst 6:303–322

    Google Scholar 

  • Dennison SJ, O'Connor BL, Aprison MH, Merritt VE, Felten DL (1981): Viscerotopic localisation of preganglionic parasympathetic cell bodies of origin of the anterior and posterior subdiaphragmatic vagus nerves. J Comp Neurol 197:259–269

    Google Scholar 

  • Gruber H (1968) Über Struktur und Innervation der quergestreiften Muskulator des Oesophagus der Ratte. Z Zellforsch 91:236–247

    Google Scholar 

  • Gwyn DG, Leslie RA, Hopkins DA (1979) Gastric afferents to the nucleus of the solitary tract in the cat. Neurosci Lett 14:13–17

    Google Scholar 

  • Hinrichsen CFL, Ryan AT (1981) Localization of laryngeal motoneurons in the rat: morphologic evidence for dual innervation. Exp Neurol 74:341–355

    Google Scholar 

  • Kalia M, Mesulam MM (1980a) Brain stem projections of sensory and motor components of the vagus complex in the cat: 1. The cervical vagus and nodose ganglion. J Comp Neurol 193:435–465

    Google Scholar 

  • Kalia M, Mesulam MM (1980b) Brain stem projections of sensory and motor components of the vagus complex in the cat: II. Laryngeal, tracheobronchial, pulmonary, cardiac and gastrointestinal branches. J Comp Neurol 193:467–508

    Google Scholar 

  • Kalia M, Sullivan JM (1982) Brainstem projections of sensory and motor components of the vagus nerve in the rat. J Comp Neurol 211:248–264

    Google Scholar 

  • Karim MA, Leong SK, Perwaiz SA (1981) On the anatomical organization of the vagal nuclei. Am J Primatol 1:277–292

    Google Scholar 

  • Katan S, Gottschall J, Neuhuber W (1982) Simultaneous visualization of horseradish peroxidase and nuclear yellow in tissue sections for neuronal double labeling. Neurosci Lett 28:121–126

    Google Scholar 

  • Katz DM, Karten HJ (1983) Visceral representation within the nucleus of the tractus solitarius in the pigeon, Columba livia. J Comp Neurol 218:42–73

    Google Scholar 

  • Leek BF (1977) Abdominal and pelvic visceral receptors. Brain Med Bull 33:163–168

    Google Scholar 

  • Mei N (1983) Sensory structures in the viscera. In: Ottoson D (ed) Progress in sensory physiology 4. Springer, Berlin, Heidelber, New York, Tokyo, 1–42

    Google Scholar 

  • Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reactionproduct with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:2:106–117

    Google Scholar 

  • Neuhuber W, Niederle B (1979) Spinal ganglion cells innervating the stomach of the rat as demonstrated by somatopetal transport of horseradish peroxidase (HRP). Anat Embryol 155:355–362

    Google Scholar 

  • Nomura S, Mizuno N (1983) Central distribution of efferent and afferent components of the cervical branches of the vagus nerve. Anat Embryol 166:1–18

    Google Scholar 

  • Paintal AS (1973) Vagal sensory receptors and their reflex effects. Physiol Rev 53:159–221

    Google Scholar 

  • Pásaro R, Lobera B, Gonzáles-Barón S, Delgado-Garcia JM (1981) Localización de las motoneuronas de los músculos intrinsecos de la laringe en la rata. Rev Españ Fisiol 37:317–322

    Google Scholar 

  • Robles-Chillida EM, Rodrigo J, Mayo I, Arnedo A, Gomez A (1981) Ultrastructure of free-ending nerve fibers in oesophageal epithelium. J Anat 133:227–233

    Google Scholar 

  • Rogers RC, Hermann GE (1983) Central connections of the hepatic branch of the vagus nerve: a horseradish peroxidase histochemical study. J Autonom Nerv Syst 7:165–174

    Google Scholar 

  • Takayama K, Ishikawa N, Miura M (1982) Sites of origin and termination of gastric vagus preganglionic neurons: an HRP study in the rat. J Autonom Nerv Syst 6:211–223

    Google Scholar 

  • Torvik A (1956) Afferent connections to the sensory trigeminal nuclei, the nucleus of the solitary tract, and adjacent structures. J Comp Neurol 106:51–141

    Google Scholar 

  • Wan-Hua AY (1980) Uptake sites of horseradish peroxidase after injection into peritoneal structures: defining some pitfalls. J Neurosci Meth 2:123–133

    Google Scholar 

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Author notes

  1. D. Kantner

    Present address: Anatomisches Institut, Winterthurerstr. 190, CH-8057, Zürich

Authors and Affiliations

  1. Anatomical Institute, University of Zürich, Switzerland

    T. Fryscak, W. Zenker & D. Kantner

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  1. T. Fryscak
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  2. W. Zenker
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  3. D. Kantner
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Fryscak, T., Zenker, W. & Kantner, D. Afferent and efferent innervation of the rat esophagus. Anat Embryol 170, 63–70 (1984). https://doi.org/10.1007/BF00319459

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