Abstract
The in vivo interaction of the lectin Ulex europaeus agglutinin 1 with mouse Peyer's patch follicle-associated epithelial cells was studied in the mouse Peyer's patch gut loop model by immunofluorescence and electron microscopy. The lectin targets to mouse Peyer's patch M-cells and is rapidly endocytosed and transcytosed. These processes are accompanied by morphological changes in the M-cell microvilli and by redistribution of polymerised actin. The demonstration of selective binding and uptake of a lectin by intestinal M-cells in vivo suggests that M-cell-specific surface glycoconjugates might act as receptors for the selective adhesion/uptake of microorganisms.
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This work was supported under the LINK programme in Selective Drug Delivery and Targeting, funded by SERC/MRC/DTI and industry (SERC grant GR/F 09747). M.A.J. was also supported by a Wellcome Postdoctoral Research Followship (039684/Z/93/Z). Additional support was provided by the Royal Society for equipment.
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Clark, M.A., Jepson, M.A., Simmons, N.L. et al. Selective binding and transcytosis of Ulex europaeus 1 lectin by mouse Peyer's patch M-cells in vivo. Cell Tissue Res 282, 455–461 (1995). https://doi.org/10.1007/BF00318877
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DOI: https://doi.org/10.1007/BF00318877