Skip to main content
SpringerLink
Log in

The presence and distribution of Antho-RFamide-like material in scyphomedusae

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The nervous systems of the scyphomedusae Chrysaora hysoscella, Cyanea capillata and Cyanea lamarckii (Phylum Cnidaria) were stained using an antiserum against the anthozoan neuropeptide Antho-RFamide. Staining was widespread in all three species. In Chrysaora, the antiserum revealed nerve nets in the subumrella and exumbrella ectoderm, in both faces of the oral lobes, and in the endoderm lining the subumbrella and exumbrella surfaces of the gastric cavity. The most prominent staining occurred in a dense plexus of neurons in the ectoderm at the base of the tentacles. This nerve net sent projections into the subumbrella ectoderm. For the most part, staining in the two species of Cyanea was similar to that in Chrysaora, with a few exceptions. These include the presence, in Cyanea, of an obvious tentacular nerve tract and nerve nets associated with clusters of cnidocytes in the tentacles. Radioimmunoassays of extracts from Chrysaora and Cyanea lamarkii revealed that both species contain large amounts of Antho-RFamide-like material (up to 55 nmol/animal). The results indicate that Antho-RFamide-like neuropeptides are widespread in scyphomedusae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aerne BL, Stidwell RP, Tardent P (1991) Nematocyst discharge in Hydra does not require the presence of nerve cells. J Exp Zool 258:137–141

    Google Scholar 

  • Anderson PAV, Schwab WE (1981) The organization and structure of nerve and muscles in the jellyfish Cyanea capillata (Coelenterata: Scyphozoa). J Morphol 170:383–399

    Google Scholar 

  • Anderson PAV, Schwab WE (1983) Action potentials in neurons of the motor nerve net of Cyanea (Coelenterata). J Neurophysiol 50:671–683

    Google Scholar 

  • Bozler E (1927) Untersuchungen über das Nervensystem der Coelenteraten. I Teil: Kontinuität oder Kontakt zwischen den Nervenzellen? Z Zellforsch Mikrosk Anat 5:244–262

    Google Scholar 

  • Claus C (1877) Studien über Polypen und Quallen der Adria. I. Acalephen (Discomedusen). Denkschr mathem naturwiss Classe kaiserl Acad Wissenschaften Wien 38:1–64

    Google Scholar 

  • Eimer T (1878) Die Medusen physiologisch und morphologisch auf ihr Nervensystem untersucht. Laupp, Tübingen

    Google Scholar 

  • Graff D, Grimmelikhuijzen CJP (1988a) Isolation of <Glu-Ser-Leu-Arg-Trp-NH2, a novel neuropeptide from sea anemones. Brain Res 442:354–358

    Google Scholar 

  • Graff D, Grimmelikhuijzen CJP (1988b) Isolation of <Glu-Gly-Leu-Arg-Trp-NH2 (Antho-RWamide II), a novel neuropeptide from sea anemones. FEBS Lett 239:137–140

    Google Scholar 

  • Grimmelikhuijzen CJP (1983) FMRFamide immunoreactivity is generally occurring in the nervous systems of coelenterates. Histochemistry 78:361–381

    Google Scholar 

  • Grimmelikhuijzen CJP (1985) Antisera to the sequence Arg-Phe-amide visualize neuronal centralization in hydroid polyps. Cell Tissue Res 241:171–182

    Google Scholar 

  • Grimmelikhuijzen CJP, Graff D (1986) Isolation of <Glu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide from sea anemones. Proc Natl Acad Sci USA 83:9817–9821

    Google Scholar 

  • Grimmelikhuijzen CJP, Groeger A (1987) Isolation of the neuropeptide pGlu-Gly-Arg-Phe-amide from the pennatulid Renilla köllikeri. FEBS Lett 211:105–108

    Google Scholar 

  • Grimmelikhuijzen CJP, Spencer AN (1984) FMRF-amide immunoreactivity in the nervous system of the medusa Polyorchis penicillatus. J Comp Neurol 230:361–371

    Google Scholar 

  • Grimmelikhuijzen CJP, Spencer AN, Carré D (1986) Organization of the nervous system of physonectid siphonophores. Cell Tissue Res 246:463–479

    Google Scholar 

  • Grimmelikhuijzen CJP, Graff D, Spencer AN (1988a) Structure, location and possible actions of Arg-Phe-amide peptides in coelenterates. In: Thorndyke MC, Goldsworthy GJ (eds) Neurohormones in invertebrates. Cambridge University Press, Cambridge, pp 199–217

    Google Scholar 

  • Grimmelikhuijzen CJP, Hahn M, Rinehart KL, Spencer AN (1988b) Isolation of <Glu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide), a novel neuropeptide from hydromedusae. Brain Res 475:198–203

    Google Scholar 

  • Grimmelikhuijzen CJP, Graff D, McFarlane ID (1989a) Neurones and neuropeptides in coelenterates. Arch Histol Cytol 52 [Suppl]:265–276

    Google Scholar 

  • Grimmelikhuijzen CJP, Graff D, Koizumi O, Westfall JA, McFarlane ID (1989b) Neurons and their peptide transmitters in coelenterates. In: Anderson PAV (ed) Evolution of the first nervous systems. Plenum, New York, pp 95–109

    Google Scholar 

  • Grimmelikhuijzen CJP, Rinehart KL, Jacob E, Graff D, Reinscheid RK, Nothacker H-P, Staley AL (1990) Isolation of L-3-phenyllacty-Leu-Arg-Asn-NH2 (Antho-RNamide), a sea anemone neuropeptide containing and unusual amino-terminal blocking group. Proc Natl Acad Sci USA 87:5410–5414

    Google Scholar 

  • Hertwig O, Hertwig R (1878) Das Nervensystem und die Sinnesorgane der Medusen. Vogel, Leipzig

    Google Scholar 

  • Hesse R (1895) Über das Nervensystem und die Sinnesorgane von Rhizostoma cuvieri. Zeitschr wissensch Zool 60:411–457

    Google Scholar 

  • Horridge GA (1954) Observations on the nerve fibers of Aurelia aurita. Quart J Microsc Res 95:85–92

    Google Scholar 

  • Horridge A (1956) The nervous system of the ephyra larva of Aurelia aurita. Quart J Microsc Res 97:59–74

    Google Scholar 

  • Koizumi O, Wilson JD, Grimmelikhuijzen CJP, Westfall JA (1989) Ultrastructural organization of RFamide-like peptides in neuronal dense-cored vesicles in the peducle of Hydra. J Exp Zool 249:17–22

    Google Scholar 

  • Lendenfeld R von (1882) Über Coelenteraten der Südsee. I. Mittheilung. Cyanea Annaskala nov. sp. Zeitschr Wissensch Zool 37:465–552

    Google Scholar 

  • Lendenfeld R von (1888) Über Coelenteraten der Südsee. VII. Mittheilung. Die australische rhizostomonen Medusen. Zeitchr Wissensch Zool 47:201–324

    Google Scholar 

  • Mackie GO, Singla CL, Stell WK (1985) Distribution of nerve elements showing FMRFamide-like immunoreactivity in hydromedusae. Acta Zool Stockh 66:199–210

    Google Scholar 

  • McFarlane ID, Grimmelikhuijzen CJP (1991) Three anthozoan neuropeptides Antho-RFamide and Antho-RWamide I and II modulate spontaneous tentacle contractions in sea anemones. J Exp Biol 155:669–673

    Google Scholar 

  • McFarlane ID, Graff D, Grimmelikhuijzen CJP (1987) Excitatory actions of Antho-RFamide, an anthozoan neuropeptide, on muscles and conducting systems in the sea anemone Calliactis parasitica. J Exp Biol 133:157–168

    Google Scholar 

  • McFarlane ID, Anderson PAV, Grimmelikhuijzen CJP (1991) Effects of three anthozoan neuropeptides, Antho-RWamide I and II and Antho-RFamide, on slow muscles from sea anemones. J Exp Biol 156:419–431

    Google Scholar 

  • Passano KN, Passano LM (1971) The endodermal nerve net of Scyphozoa. J Morphol 133:105–123

    Google Scholar 

  • Passano LM (1982) Scyphozoa and Cubozoa. In: Shelton GAB (ed) Electrical conduction and behaviour in ‘simple’ invertebrates. Clarendon, Oxford, pp 149–202

    Google Scholar 

  • Schäfer EA (1879) Observations on the nervous system of Aurelia aurita. Phil Trans R Soc Lond 169:563–575

    Google Scholar 

  • Smith SJ, Oshida J, Bode H (1974) Inhibition of nematocyst discharge in Hydra fed to repletion. Biol Bull 147:186–202

    Google Scholar 

  • Spencer AN (1988) Effects of Arg-Phe-amide peptides on identified motor neurons in the hydromedusa Polyorchis penicillatus. Can J Zool 66:649–645

    Google Scholar 

  • Weber C (1989) Smooth muscle fibers of Podocoryne carnea demonstrated by a specific monoclonal antibody and their association with neurons showing FMRFamide-like immunoreactivity. Cell Tissue Res 255:275–282

    Google Scholar 

  • Westfall JA (1973) Ultrastructural evidence for a granule-containing sensory-motor-interneuron in Hydra littoralis. J Ultrastruc Res 42:268–282

    Google Scholar 

  • Westfall JA, Kinnamon JC (1978) A second sensory-motor-interneuron with neurosecretory granules in Hydra. J Neurocytol 7:365–379

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Molecular Neurobiology, University of Hamburg, W-2000, Hamburg 20, Federal Republic of Germany

    Peter A. V. Anderson, Angelika Moosler & Cornelis J. P. Grimmelikhuijzen

  2. Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, Fl, USA

    Peter A. V. Anderson

  3. Department of Physiology, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, Fl, USA

    Peter A. V. Anderson

  4. Department of Neuroscience, University of Florida, 9505 Ocean Shore Boulevard, 32086, St. Augustine, Fl, USA

    Peter A. V. Anderson

  5. Whitney Laboratory, 9505 Ocean Shore Boulevard, 32086, St. Augustine, FL, USA

    Peter A. V. Anderson

Authors
  1. Peter A. V. Anderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Angelika Moosler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cornelis J. P. Grimmelikhuijzen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Anderson, P.A.V., Moosler, A. & Grimmelikhuijzen, C.J.P. The presence and distribution of Antho-RFamide-like material in scyphomedusae. Cell Tissue Res 267, 67–74 (1992). https://doi.org/10.1007/BF00318692

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00318692

Key words

Search

Navigation