Summary
Selected lobules of term human placenta were extracorporeally perfused and human immunoglobulin-G complexed to horseradish peroxidase (IgG-HRP) was added to the maternal perfusate. After different durations of perfusion IgG-HRP was visualised by use of diamino-benzidine cytochemistry. Within the first 10 min of perfusion IgG-HRP was found bound to microvilli and coated pits of the syncytiotrophoblast; internalisation into coated vesicles and tubulo-vesicular bodies was also observed. Subsequently, IgG-HRP was found in multivesicular bodies and by 30 min appeared in basal vesicles, the frequency of the latter event increasing with time. No routing of IgG-HRP into Golgi regions or lysosomes could be detected. By 60 min IgG-HRP was found in a few caveolae of fetal endothelium of both terminal and intermediate villi. IgG-HRP was not found in intercellular clefts of the endothelium. The pattern of uptake and routing observed suggests a receptor-mediated transcytosis of IgG-HRP across the syncytiotrophoblast and a transcellular pathway through the endothelium.
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References
Abrahamson DR, Rodewald R (1981) Evidence for the sorting of endocytic vesicle contents during the receptor-mediated transport of IgG across the newborn rat intestine. J Cell Biol 91: 270–280
Balfour AH, Jones EA (1977) The binding of plasma proteins to human placental cell-membranes. Clin Sci Mol Med 52:383
Booth AG, Wilson MJ (1981) Human placental coated vesicles contain receptor-bound transferrin. J Biochem 196:355–362
Burton DR (1985) Immunoglobulin G: functional sites. Mol Immunol 22:161–202
Contractor SF, Eaton BM (1986) Role of transferrin in iron transport between maternal and fetal circulations of a perfused lobule of human placenta. Cell Biochem Funct 4:69–74
Contractor SF, Eaton BM, Stannard PJ (1983) Uptake and fate of exogenous immunoglobulin G in the perfused human placenta. J Reprod Immunol 5:265–273
Contractor SF, Eaton BM, Firth JA, Bauman KF (1984) A comparison of effects of different perfusion regimes on the structure of the isolated human placental lobule. Cell Tissue Res 237:609–617
Contractor SF, Stannard PJ (1983) The use of AIB transport to access the suitability of a system of human perfusion for drug transport studies. Placenta 4:19–30
Douglas GC, King BF (1988) Receptor-mediated endocytosis of 125I-labelled transferrin by human choriocarcinoma (JAR) cells. Placenta 9:253–265
Einhorn MS, Granoff DM, Nahm MH, Quinn A, Shackleford PG (1987) Concentrations of antibodies in paired maternal and infant sera: relationship to IgG subclass. J Pediatr 111:783–788
Galbraith GMP, Galbraith RA, Page Faulk W (1980) Immunological studies of transferrin and transferrin receptors of human placental trophoblast. Placenta 1:33–46
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Gruenberg J, Griffiths G, Howell KE (1989) Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro. J Cell Biol 108:1301–1316
Geuze HJ, Slot JW, Strous GJAM, Peppard J, von Figura K, Hasilik A, Schwartz AL (1984) Intracellular receptor sorting during endocytosis. Comparative immunoelectron microscopy of multiple receptors in rat liver. Cell 37:195–204
Hopkins CR (1983) The importance of the endosome in intracellular traffic. Nature 304:684–685
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137 A
Kaufmann P, Bruns U, Leiser R, Luckhardt M, Winterhager E (1985) The fetal vascularisation of term human placental villi II. Intermediate and terminal villi. Anat Embryol 173:203–214
King BF (1982a) Absorption of peroxidase-conjugated immunoglobulin G by human placenta: an in vitro study. Placenta 3:395–406
King BF (1982b) The role of coated vesicles in selective transfer across yolk sac epithelium. J Ultrastruct Res 79:273–284
Kuhn LC, Kraenbuhl JP (1982) The sacrificial receptor-translocation of polymeric IgA across epithelia. Trends Biochem Sci 7:299–302
Leach L, Eaton BM, Firth JA, Contractor SF (1989) Immunogold localisation of endogenous immunoglobulin-G in ultra-thin frozen sections of the human placenta. Cell Tissue Res 257:603–607
Levick JR, Michel CC (1973) The permeability of individually perfused frog mesenteric capillaries to T1824 and T1824-albumin as evidence for a large pore system. Q J Exp Physiol 58:67–85
Lin CT (1980) Immunoelectron microscopic localization of immunoglobulin G in human placenta. J Histochem Cytochem 28:339–346
Matre R, Haugen A (1978) The placental Fc receptors studied using immune complexes of peroxidase. Scand J Immunol 8:187–193
Moskalewski S, Ptak W, Czarnik Z (1975) Demonstration of cells with IgG receptor in human placenta. Biol Neonate 26:268–273
Nakane PK, Kawaoi A (1974) Peroxidase-labelled antibody: A new method of conjugation. J Histochem Cytochem 22:1084–1091
Pastan I, Willingham MC (1985) The pathway of endocytosis. In: Pastan I, Willingham MC (ed) Endocytosis. Plenum Press, New York, pp 1–40
Pearse BMF (1982) Coated vesicles from human placenta carry ferritin, transferrin and immunoglobulin G. Proc Natl Acad Sci USA 79:451–455
Seligman PA, Schleicher RB, Allen RH (1979) Isolation and characterization of the transferrin receptor from human placenta. J Biol Chem 256:9943–9946
Watabe H, Gitlin JI, Gitlin D (1980) Further characterization of IgG receptors from human placenta. J Biochem 87:393–397
Wood G, Reynard J, Krishman E, Racela L (1987) Immunobiology of the human placenta I. IgG Fc receptors in trophoblastic villi. Cell Immunol 35:191–204
Wild AE (1981) Endocytic mechanisms in protein transfer across the placenta. Placenta [Suppl] 1:165–186
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Leach, L., Eaton, B.M., Firth, J.A. et al. Uptake and intracellular routing of peroxidase-conjugated immunoglobulin-G by the perfused human placenta. Cell Tissue Res 261, 383–388 (1990). https://doi.org/10.1007/BF00318681
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DOI: https://doi.org/10.1007/BF00318681