Summary
The distribution of mesoderm, the structure of mesoderm cells and relationship between mesoderm and ectoderm were examined by SEM in embryos at stages 3 to 5. The mesoderm was displayed by removal of the endoderm and by fracturing the embryos through mesoderm containing regions. Within the mesoderm layer four zones could be distinguished by their cell shape and arrangement — the primitive streak, a multilayered compact area around the margins of the area pellucida, multilayered and loosely arranged cells near the primitive streak and a flattened monolayer of cells around the advancing lateral and anterior edges of the mesoderm sheet. Secretion of basement membrane by the ectoderm was seen to precede the arrival of mesoderm cells. This suggests that ectoderm alone can synthesize basement membrane without mesodermal contribution.
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Abercrombie, M., Heaysman, J.E., Pegrum, S.M.: Locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Exp. Cell Res. 67, 359–367 (1971)
Bancroft, M., Bellairs, R.: Differentiation of the neutral plate and neutral tube in the young chick embryo. Anat. Embryol. 147, 309–335 (1975)
Bard, J.B.L., Hay, E.D., Meller, S.D.: Formation of the endothelium of the avian cornea: A study of cell movement in vivo. Devel. Biol. 42, 334–361 (1975)
Bellairs, R.: Developmental processes in higher vertebrates, 1st edition. London: Logos Press 1971
Cohen, A.M., Hay, E.D.: Secretion of collagen by embryonic neuroepithelium at the time of spinalcord-somite interaction. Devel. Biol. 26, 578–605 (1971)
Dodson, J.W., Hay, E.D.: Secretion of collagenous stroma by isolated epithelium grown in vitro. Exp. Cell Res. 65, 215–220 (1971)
Hamburger, V., Hamilton, H.L.: A series of normal stages in the development of the chick embryo. J. Morph. 88, 49–92 (1951)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A (1965)
New, D.A.T.: A new technique for the cultivation of the chick embryo in vitro. J. Embryol. exp. Morphol. 3, 326–331 (1955)
Newsome, D.A., Kenyon, K.R.: Collagen production in vitro by the retinal pigment epithelium. Develop. Biol. 32, 387–400 (1973)
Revel, J.P.: Some aspects of cellular interactions in development (ed. A.A. Moscona) The cell surface in development, 1st edition. New York: John Wiley & Sons, Inc. 1974
Rosenquist, G.C.: A radioautographic study of labelled grafts in the chick blastoderm development from the primitive streak stages to stage 12. Contr. Embryol. Carneg. Instn. 38, 71–110 (1966)
Spratt Jr., N.T.: Formation of the primitive streak in the explanted chick blastoderm marked with carbon particles. J. Exp. Zool. 103, 259–304 (1946)
Silver, P.H.S., Wakely, J.: Fine structure origin and fate of extracellular materials in the interspace between the presumptive lens and presumptive retina of the chick embryo. J. Anat. 118, 19–31 (1974)
Trelstad, R.L., Hay, E.D., Revel, J.P.: Cell contact during early morphogenesis in the chick embryo. Devel. Biol. 16, 78–106 (1967)
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England, M.A., Wakely, J. Scanning electron microscopy of the development of the mesoderm layer in chick embryos. Anat. Embryol. 150, 291–300 (1977). https://doi.org/10.1007/BF00318347
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DOI: https://doi.org/10.1007/BF00318347