Summary
Expression of pho1-encodd acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses this activity. Full repression by adenine only occurs when phosphate is not growth limiting and vice versa. Regulation occurs at the level of mRNA. We isolated adenine non-repressible mutants. They define four genes (anr1, anr2, anr3, and anr5) which are involved in adenine-dependent pho1 expression. All anr mutants are also phosphate nonrepressible. These results indicate that the generation and/or transduction of the intracellular signal responsible for pho1 repression is simultaneously dependent on both adenine and phosphate.
Similar content being viewed by others
References
Dhamija SS, Fluri R, Schweingruber ME (1986) Curr Genet 11:467–473
Elliot S, Chang C, Schweingruber ME, Schaller J, Rickli EF, Carbon J (1986) J Biol Chem 261:2936–2941
Gutz H, Heslot H, Leupold U, Loprieno N (1974) In: King RC (ed) Handbook of genetics, vol 1. Plenum Press, New York, pp 395–446
Maundrell K, Nurse P, Schönholzer F, Schweingruber ME (1985) Gene 39:223–230
Schweingruber AM, Dlugonski J, Edenharter E, Schweingruber ME (1991) Curr Genet 19:249–254
Schweingruber AM, Fankhauser H, Dlugonski J, Steinmann-Loss C, Schweingruber ME (1992) Genetics 30:445–449
Schweingruber ME (1987) Adv Prot Phosphatases 4:77–93
Schweingruber ME, Edenharter E (1990) Curr Genet 17:191–194
Schweingruber ME, Fluri R, Maundrell K, Schweingruber AM, Dumermuth E (1986) J Biol Chem 261:15877–15882
Schweingruber ME, Schweingruber AM (1981) Differentiation 19:68–70
Schweingruber ME, Schweingruber AM, Schüpbach ME (1982) Curr Genet 5:109–117
Yang J, Schweingruber ME (1990) Curr Genet 18:269–272
Zurlinden A, Schweingruber ME (1992) Gene (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schweingruber, M.E., Edenharter, E., Zurlinden, A. et al. Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate. Curr Genet 22, 289–292 (1992). https://doi.org/10.1007/BF00317923
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00317923