Summary
We examined how combinations of parentage, fungicide application, and artificial herbivory influence growth and shoot phosphorus content in pre-reproductive Lotus corniculatus, using young offspring arising from three parental crosses, two of which had one parent in common. Soil with vesicular-arbuscular mycorrhizal (VAM) fungi was treated with either water or benomyl, an anti-VAM fungicide, and added to trays containing groups of four full siblings. There were two experiments; in the first no plants were clipped while in the second two of the four plants were clipped to simulate herbivory. In both experiments plants of the two related crosses accumulated more biomass and total shoot P than did plants of the third cross. Plants inoculated with watertreated soil had greater shoot mass and P concentration than did fungicide-treated replicates but the extent of increase in P concentration varied among crosses. In Experiment 2, clipping reduced root mass and resulted in higher shoot P concentration. In this experiment there was a significant interaction of fungicide application and clipping: both unclipped and clipped plants grew better in soil not treated with fungicide, but the increase in shoot mass, total mass, and total P was greater in unclipped plants. Significant interaction of fungicide treatment and clipping is most likely due to reduced availability of carbon to the roots of clipped plants, resulting in poorer symbiotic functioning.
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References
Allen EB, Allen MF (1986) Water relations of xeric grasses in the field: interactions of mycorrhizas and competition. New Phytol 104:559–571
Anderson RC, Liberta AE (1989) Growth of little bluestem (Schizachyrium scoparium) (Poaceae) in fumigated and nonfumigated soils under various inorganic nutrient conditions. Am J Bot 76:95–104
Carpenter AT, Allen MF (1988) Responses of Hedysarum boreale Nutt. to mycorrhizas and Rhizobium: plant and soil nutrient changes in a disturbed shrub-steppe. New Phytol 109:125–132
Chiariello N, Hickman JC, Mooney HA (1982) Endomycorrhizal role for interspecific transfer of phosphorus in a community of annual plants. Science 217:941–943
Daft MJ, El-Giamhi AA (1978) Effect of arbuscular mycorrhiza on plant growth. VII. Effects of defoliation and light on selected hosts. New Phytol 80:365–372
Finlay RD (1985) Interactions between soil micro-arthropods and endomycorrhizal associations of higher plants. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil. British Ecological Society Special Publ. No. 4, Blackwell, Oxford
Fitter AH (1985) Functioning of vesicular-arbuscular mycorrhizas under field conditions. New Phytol 99:257–265
Fitter AH (1986) Effect of benomyl on leaf phosphorus concentration in alpine grasslands: a test of mycorrhizal benefit. New Phytol 103:767–776
Fitter AH (1989) The role and ecological significance of vesicular-arbuscular mycorrhizas in temperate ecosystems. In: Mejstrik V (ed) Proceedings of the Second European Symposium on Mycorrhiza. Academic, Prague (in press)
Fitter AH, Nichols R (1988) The use of benomyl to control infection by vesicular-arbuscular mycorrhizal fungi. New Phytol 110:201–206
Francis R, Read DJ (1984) Direct transfer of carbon between plants connected by vesicular-arbuscular mycorrhizal mycelium. Nature 307:53–56
Francis R, Finlay RD, Read DJ (1986) Vesicular-arbuscular mycorrhiza in natural vegetation systems. New Phytol 102:103–111
Grime JP, Mackey JML, Hillier SH, Read DJ (1987) Floristic diversity in a model system using experimental microcosms. Nature 328:420–422
Hale MG, Sanders FE (1982) Effects of benomyl on vesicular-arbuscular mycorrhizal infection of red clover (Trifolium pratense L.) and consequences for phosphorus inflow. J Plant Nutrition 5:1355–1367
Hendrix S (1988) Herbivory and its impact on plant reproduction. In: Lovett Doust J, Lovett Doust L (eds) Plant reproductive ecology: patterns and strategies. Oxford University Press, London, pp 246–263
Huang R-S, Smith WK, Yost RS (1985) Influence of vesicular-arbuscular mycorrhiza on growth, water relations, and leaf orientation in Leucaena leucocephala (Lam.) DeWit. New Phytol 99:229–243
Janos DP (1980) Vesicular-arbuscular mycorrhizae affect lowland tropical rain forest plant growth. Ecology 61:151–162
Jones DA, Turkington R (1986) Biological flora of the British Isles. No. 163. Lotus corniculatus. J Ecol 74:1185–1212
Lambert DH, Baker D, Cole H Jr (1979) The role of mycorrhizae in the interactions of phosphorus with zinc, copper, and other elements. J Soil Sci Soc Am 43:976–980
Newman EI (1988) Mycorrhizal links between plants: their functioning and ecological significance. Adv Ecol Res 18:243–270
Read DJ, Francis R, Finlay RD (1985) Mycorrhizal mycelia and nutrient cycling in plant communities. In: Fitter AH (ed) Ecological interactions in soil. Blackwell Scientific Publications, London, pp 193–217
Rhodes LH, Gerdemann JW (1978) Hyphal translocation and uptake of sulfur by vesicular-arbuscular mycorrhizae of onion. Soil Biol Biochem 10:355–360
Smith SE, St. John BJ, Smith FA, Bromley J-L (1986) Effects of mycorrhizal infection on plant growth, nitrogen and phosphorus nutrition in glasshouse-grown Allium cepa L. New Phytol 103:359–373
Snedecor GW, Cochran WG (1976) Statistical Methods. Iowa State University Press, Ames, Iowa
Sokal RR, Rohlf FJ (1981) Biometry. 2nd edition. W.H. Freeman and Company, New York
SPSS, Inc (1983) SPSSx user's guide. McGraw-Hill Book Co., New York
Stephenson AG (1984) The regulation of maternal investment in an indeterminate flowering plant (Lotus corniculatus). Ecology 65:113–121
Verkade SD, Hamilton DF (1983) Effects of benomyl on growth of Liriodendron tulipifera L. seedlings inoculated with the vesicular-arbuscular fungus Glomus fasciculatus. Sci Horticul 21:253–260
Wallace LL (1981) Growth, morphology and gas exchange of mycorrhizal and non-mycorrhizal Panicum coloratum L., a C4 grass species, under different clipping and fertilization regimes. Oecologia 49:272–278
Wallace LL (1987) Mycorrhizas in grasslands: interactions of ungulates, fungi and drought. New Phytol 105:619–632
Wallace LL, Svejcar T (1987) Mycorrhizal and clipping effects on Andropogon gerardii photosynthesis. Am J Bot 74:1138–1142
Wallace LL, McNaughton SJ, Coughenour MB (1982) The effects of clipping and fertilization on nitrogen nutrition and allocation by mycorrhizal and non-mycorrhizal Panicum coloratum L., a C4 grass. Oecologia 54:68–71
Whittingham J, Read DJ (1982) Vesicular-arbuscular mycorrhiza in natural vegetation systems. III. Nutrient transfer between plants with mycorrhizal inter-connections. New Phytol 90:277–284
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Borowicz, V.A., Fitter, A.H. Effects of endomycorrhizal infection, artificial herbivory, and parental cross on growth of Lotus corniculatus L.. Oecologia 82, 402–407 (1990). https://doi.org/10.1007/BF00317489
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DOI: https://doi.org/10.1007/BF00317489