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Galanin and vasoactive intestinal polypeptide are colocalised with classical pituitary hormones and show plasticity of expression

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Summary

The identity of galanin- and vasoactive intestinal polypeptide-(VIP) immunoreactive (IR) cells in the rat anterior pituitary was investigated using immunocytochemistry and, since levels of both peptides are stimulated by oestrogen, the effect of oestrogen treatment and gonadectomy on the expression of both peptides was examined. In normal male rats, few galanin-IR and very few VIP-IR cells were found. Colocalisation studies performed on 2-μm serial paraffin sections revealed that in these animals galanin IR was present in somatotrophs and thyrotrophs. In normal females in dioestrus many lactotrophs, in addition to somatotrophs and thyrotrophs, expressed galanin, but very few VIP-IR cells were seen. In cryostat sections of normal rat pituitaries, slightly more VIP-IR cells were present. Oestrogen treatment in females produced an increase in frequency of galanin-IR cells, the vast majority of which were lactotrophs, and more VIP-IR cells, identified as lactotrophs, also appeared. VIP was present in a subset of galanin-IR lactotrophs after oestrogen treatment. After ovariectomy female pituitaries resembled those of normal males, with few galanin positive cells none of which were lactotrophs, and hardly any VIP-IR cells. Thus these two peptides are present in specific endocrine cell types of rat anterior pituitary and display plasticity of expression in different cell types under the influence of oestrogen. Their roles in control of pituitary hormone secretion are supported by these findings, and it is possible that both peptides act in a paracrine fashion within the pituitary.

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References

  • Abrams GM, Nilaver G, Zimmerman EA (1985) VIP-containing neurons. In: Hökfelt T, Björklund A (eds) Handbook of chemical neuroanatomy, vol 4. Elsevier, Amsterdam, pp 335–354

    Google Scholar 

  • Arnaout MA, Garthwaite TL, Martinson DR, Hagen TC (1986) Vasoactive intestinal polypeptide is synthesised in anterior pituitary tissue. Endocrinology 119:2052–2057

    Google Scholar 

  • Baes M, Allaerts W, Denef C (1987) Evidence for functional communication between folliculostellate cells and hormone-secreting cells in perifused anterior pituitary cell aggregates. Endocrinology 120:685–691

    Google Scholar 

  • Bauer FE, Ginsberg L, Venetikou M, Mackay DJ, Burrin JM, Bloom SR (1986) Growth hormone release in man induced by galanin, a new hypothalamic peptide. Lancet 2:192–195

    Google Scholar 

  • Black IB, Adler JE, La Gamma EF (1988) Neurotransmitter plasticity in the peripheral nervous system. In: Björklund A, Hökfelt T, Owman C (eds) Handbook of chemical neuroanatomy, vol 6. Elsevier, Amsterdam, pp 51–64

    Google Scholar 

  • Boockfor FR, Hoeffler JP, Frawley LS (1986) Estradiol induces a shift in cultured cells that release prolactin or growth hormone. Am J Physiol 250:E103-E105

    Google Scholar 

  • Ch'ng JLC, Christofides ND, Anand P, Gibson SJ, Allen YS, Su HC, Tatemoto K, Morrison JFB, Polak JM, Bloom SR (1985) Distribution of galanin immunoreactivity in the central nervous system and the response of galanin-containing neuronal pathways to injury. Neuroscience 16:343–354

    Google Scholar 

  • Conti A, Togni E, Travaglini P, Muratori M, Faglia G (1987) Vasoactive intestinal polypeptide and dopamine. Effect on prolactin secretion in normal women and patients with microprolactinomas. Neuroendocrinology 46:241–245

    Google Scholar 

  • DeFesi CR, Astier HS, Surks MI (1979) Kinetics of thyrotrophs and somatotrophs during development of hypothyroidism and L-triiodothyronine treatment of hypothyroid rats. Endocrinology 104:1172–1180

    Google Scholar 

  • Denef C, Andries M (1983) Evidence for paracrine interaction between gonadotrophs and lactotrophs in pituitary cell aggregates. Endocrinology 112:813–822

    Google Scholar 

  • Frawley LS, Boockfor FR, Hoeffler JP (1985) Identification by plaque assays of a pituitary cell type that secretes both growth hormone and prolactin. Endocrinology 116:734–737

    Google Scholar 

  • Gabriel SM, Kaplan LM, Martin JB (1988a) Sex differences in galanin gene expression during development in the rat. American Endocrine Society Abstract No. 1169

  • Gabriel SM, Milbury CM, Nathanson JA, Martin JB (1988b) Galanin stimulates rat pituitary growth hormone secretion in vitro. Life Sci 42:1981–1986

    Google Scholar 

  • Gibson SJ, Polak JM, Anand P, Blank MA, Yiangou Y, Su HC, Terenghi G, Katagiri G, Morrison JFB, Lumb BM, Inyama C, Bloom SR (1986) A VIP/PHI-containing pathway links urinary bladder and sacral spinal cord. Peptides 7 (Suppl 1):205–219

    Google Scholar 

  • Gourdji D, Bataille D, Vauclin N, Grouselle D, Rosselin G, Tixier-Vidal A (1979) Vasoactive intestinal polypeptide (VIP) stimulates prolactin (PRL) release and cAMP production in rat pituitary cell line (GH3/B6). Additive effects of VIP and TRH on PRL release. FEBS Lett 104:165–168

    Google Scholar 

  • Gozes I, Werner H, Fawzi M, Abdelatty A, Shani Y, Fridkin M, Koch Y (1989) Estrogen regulation of vasoactive intestinal peptide mRNA in rat hypothalamus. J Mol Neurosci 1:55–61

    Google Scholar 

  • Hoeffler JP, Frawley LS (1987) Hypothalmic releasing factors differentially affect the proportions of cells that secrete growth hormone or prolactin. Endocrinology 120:791–795

    Google Scholar 

  • Horvath E, Kovacs K (1988) Fine structural cytology of the adenohypophysis in rat and man. J Electron Microsc Tech 8:401–432

    Google Scholar 

  • Hsu SM, Soban E (1982) Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem 30:1079–1082

    Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) The use of antiavidin antibody and avidin-biotin-perioxidase complex in immunoperoxidase technics. Am J Clin Pathol 75:816–821

    Google Scholar 

  • Huang WM, Gibson SJ, Facer P, Gu J, Polak JM (1983) Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating. Histochemistry 77:275–279

    Google Scholar 

  • Inoue T, Kato Y, Koshiyama H, Yanaihara N, Imura H (1988) Galanin stimulates the release of vasoactive intestinal polypeptide from perifused hypothalamic fragments in vitro and from periventricular structures into the cerebrospinal fluid in vivo in the rat. Neurosci Lett 85:95–100

    Google Scholar 

  • Kaplan LM, Gabriel SM, Koenig JI, Sunday ME, Spindel ER, Martin JB, Chin WW (1988) Galanin is an oestrogen-inducible secretory product of the rat anterior pituitary. Proc Natl Acad Sci USA 85:7408–7412

    Google Scholar 

  • Kato Y, Iwasaki J, Abe H, Yanaihara N, Imura H (1978) Prolactin release by vasoactive intestinal polypeptide in rats. Endocrinology 103:554–558

    Google Scholar 

  • Koshiyama H, Kato Y, Inoue T, Murakami Y, Ishikawa Y, Yanaihara N, Imura H (1987) Central galanin stimulates pituitary prolactin secretion in rats: possible involvement of hypothalamic vasoactive intestinal polypeptide. Neurosci Lett 75:49–54

    Google Scholar 

  • Lam KSL, Minimatani N, Reichlin S (1988) Post-translational processing of pre-pro-VIP (vasoactive intestinal polypeptide) in the rat anterior pituitary — effects of thyroid hormone and estrogen. 8th International Congress of Endocrinology, Kyoto, Japan (Abstract)

  • Lam KSL, Lechan RM, Minimatani N, Segerson TP, Reichlin S (1989) Vasoactive intestinal peptide in the anterior pituitary is increased in hypothyroidism. Endocrinology 124:1077–1084

    Google Scholar 

  • Landis SC (1988) Neurotransmitter plasticity in sympathetic neurons and its regulation by environmental factors in vitro and in vivo. In: Björklund A, Hökfelt T, Owman C (eds) Handbook of chemical neuroanatomy, vol 6. Elsevier, Amsterdam, pp 65–115

    Google Scholar 

  • Larsson L-I (1982) Localization of vasoactive intestinal polypeptide: a critical appraisal. In: Said SI (ed) Vasoactive intestinal polypeptide. Raven Press, New York, pp 51–63

    Google Scholar 

  • Melander T, Hökfelt T, Rökaeus A, Fahrenkrug J, Tatemoto K, Mutt V (1985) Distribution of galanin-like immunoreactivity in the gastro-intestinal tract of several mammalian species. Cell Tissue Res 239:253–270

    Google Scholar 

  • Melander T, Fuxe K, Harfstrand A, Eneroth P, Hökfelt T (1987) Effects of intraventricular injections of galanin on neuroendocrine functions in the male rat. Possible involvement of hypothalamic catecholamine neuronal systems. Acta Physiol Scand 131:25–32

    Google Scholar 

  • Morel G, Besson J, Rosselin G, Dubois PM (1982) Ultrastructural evidence for endogenous vasoactive intestinal peptide-like immunoreactivity in the pituitary gland. Neuroendocrinology 34:85–89

    Google Scholar 

  • Murakami Y, Kato Y, Koshiyama H, Inoue T, Yanaihara N, Imura H (1987) Galanin stimulates growth hormone (GH) secretion via GH-releasing factor (GRF) in conscious rats. Eur J Pharmacol 136:415–418

    Google Scholar 

  • Mutt V, Said SI (1974) Structure of the porcine vasoactive intestinal polypeptide octapeptide. The amino-acid sequence. Use of kallikrein in its determination. Eur J Biochem 42:581–589

    Google Scholar 

  • Nagy G, Mulcahey J, O'Neill JD (1988) Autocrine control of prolactin secretion by vasoactive intestinal peptide. Endocrinology 122:364–366

    Google Scholar 

  • Nikitovich-Winer BA, Atkin J, Maley BE (1987) Colocalisation of prolactin and growth hormone within specific adenohypophyseal cells in male, female and lactating rats. Endocrinology 121:625–630

    Google Scholar 

  • O'Halloran DJ, Ghatei MA, Burrin JM, Steel JH, Polak JM, Bloom SR (1988a) The effect of ovariectomy and oestrogen replacement on the anterior pituitary peptides 7B2 and galanin in the rat. Regul Peptides 22:425 (Abstract)

    Google Scholar 

  • O'Halloran DJ, Jones P, Steel JH, Ghatei MA, Polak JM, Bloom SR (1988b) Modulation of hypothalamic and anterior pituitary galanin gene expression by oestrogen in the rat. J Endocrinol 119 (Suppl): Abstract 142

    Google Scholar 

  • Ottlecz A, Samson WK, McCann SM (1986) Galanin: evidence for a hypothalamic site of action to release growth hormone. Peptides 7:51–53

    Google Scholar 

  • Palkovits M, Rökaeus A, Antoni FA, Kiss A (1987) Galanin in the hypothalamo-hypophyseal system. Neuroendocrinology 46:417–423

    Google Scholar 

  • Phelps CJ (1986) Immunocytochemical analysis of prolactin cells in the adult rat adenohypohysis: distribution and quantitation relative to sex and strain. Am J Anat 176:233–242

    Google Scholar 

  • Sawchenko PE, Swanson LW, Vale WW (1984) Co-expression of corticotropin-releasing factor and vasopressin immunoreactivity in parvocellular neurosecretory neurons of the adrenalectomised rat. Proc Natl Acad Sci USA 81:1883–1887

    Google Scholar 

  • Skofitsch G, Jacobowitz DM (1986) Quantitative distribution of galanin-like immunoreactivity in the rat central nervous system. Peptides 7:609–613

    Google Scholar 

  • Steel JH, Van Noorden S, Ballesta J, Gibson SJ, Ghatei MA, Burrin J, Leonhardt U, Burrin J, Bloom SR, Polak JM (1988) Localization of 7B2, neuromedin B, and neuromedin U in specific cell types of rat, mouse and human pituitary, in rat hypothalamus, and in 30 human pituitary and extrapituitary tumors. Endocrinology 122:270–282

    Google Scholar 

  • Stratmann IE, Ezrin E, Sellers EA (1974) Estrogen-induced transformation of somatotrophs into mammotrophs in the rat. Cell Tissue Res 152:229–238

    Google Scholar 

  • Tatemoto K, Rökaeus A, Jornvall H, McDonald TJ, Mutt V (1983) Galanin — a novel biologically active peptide from porcine intestine. FEBS Lett 164:124–128

    Google Scholar 

  • Van Noorden S, Polak JM, Bloom SR, Bryant MG (1979) Vasoactive intestinal polypeptide in the pituitary pars nervosa. Neuropathol Appl Neurobiol 5:149–153

    Google Scholar 

  • Yiangou Y, Gill JS, Chrysanthou BJ, Burrin J, Bloom SR (1988) Infusion of prepro-VIP derived peptides in man: effect on secretion of prolactin. Neuroendocrinology 48:615–618

    Google Scholar 

  • Zeckwer I, Davidson LW, Keller TB, Livingood CS (1935) Pituitary in experimental cretinism: structural changes in pituitaries of thyroidectomised rats. Am J Med Sci 190:145–157

    Google Scholar 

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Authors and Affiliations

  1. Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, England

    J. H. Steel & J. M. Polak

  2. Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, England

    D. J. O'Halloran, P. M. Jones & S. R. Bloom

  3. Department of Anatomy, Jikei University School of Medicine, 105, Tokyo, Japan

    G. Gon (visiting colleague) & H. Ishikawa

  4. Department of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka, Japan

    N. Yanaihara

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  1. J. H. Steel
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  2. G. Gon
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  3. D. J. O'Halloran
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  4. P. M. Jones
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  5. N. Yanaihara
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  6. H. Ishikawa
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  7. S. R. Bloom
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  8. J. M. Polak
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Steel, J.H., Gon, G., O'Halloran, D.J. et al. Galanin and vasoactive intestinal polypeptide are colocalised with classical pituitary hormones and show plasticity of expression. Histochemistry 93, 183–189 (1989). https://doi.org/10.1007/BF00315973

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  • DOI: https://doi.org/10.1007/BF00315973

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