Summary
Swiss albino mice were injected on day 16 of pregnancy with 3H diethylstilbestrol. Fetuses were sacrificed 3h afterwards, frozen, and processed for thaw-mount autoradiography. The serial sections from 4 fetuses were studied. Intense nuclear concentration of radioactivity was found in the mesenchymal cells directly surrounding the epithelial primordia of the mammary glands, but not in the epithelium itself. Localization was similar in male and female fetuses. These findings are discussed in relation to their developmental, teratological, and oncological implications.
Similar content being viewed by others
References
Balinsky, B.I.: On the developmental process in mammary glands and other epidermal structures. Trans. Roy. Soc. Edinburgh 62, 1–31 (1949)
Cunha, G.R.: Epithelial-stromal interactions in development of the urogenital tract. Int. Rev. Cytol. 47, 137–194 (1976)
Herbst, A.L., Ulfelder, H., Poskanzer, D.C.: Adenocarcinoma of the vagina. Association of maternal stilbestrol therapy with tumor appearance in young women. N.E.J.M. 284, 878–881 (1971)
Kratochwill, K.: Development and loss of androgen responsiveness in the embryonic rudiment of the mouse mammary gland. Develop. Biol. 61, 358–365 (1977)
Kratochwill, K., Schwartz, P.: Tissue interaction in androgen response of embryonic mammary rudiment of mouse: Identification of target tissue for testosterone. Proc. Natl. Acad. Sci. USA 76, 4041–4044 (1976)
Nunez, E., Engelmann, F., Denassakag, C., Jayle, M.F.: Identification et purification préliminaire de la foetoprotéine liant les oestrogènes dans le sérum de rats nouveau-nés, C. R. Acad. Sci. (Paris) 273, 831–834 (1971)
Raynaud, A.: Observations sur les modifications provoquées par les hormones oestrogènes, du mode de développement des mamelons des foetus de Souris. C.R. Acad. Sci. 240, 674–676 (1955)
Raynaud, A.: Les mamelles, In: Traité de Zoologie, Vol. XVI, Fasc. VI, pp. 1–147. P. P.Grassé, ed. Paris: Masson et Cie (1969)
Raynaud, A., Frilley, M.: Le développement embryonnaire de la glande mammaire de la Souris, après destruction, au moyen des rayons X, des glandes génitales de l'embryon. Bull. Soc. Zool. France 74, 156–159 (1949)
Raynaud, A., Raynaud, J.: Les diverses malformations mammaires produites chez les foetus de Souris, par l'action des hormones sexuelles. C.R. Soc. Biol. Paris 148, 963–968 (1954)
Raynaud, J.P., Mercier-Bodard, C., Baulieu, E.E.: Rat estradiol binding plasma protein (EBP), Steroids, 18, 767–788 (1971)
Stumpf, W.E., Sar, M.: Autoradiographic techniques for localizing steroid hormones. In: Methods in Enzymology, Vol. XXXVI, pp. 135–156. (B.W. O'Malley and J.G. Hardman, eds.) New York: Academic Press (1975)
Stumpf, W.E., Sar, M.: Autoradiographic localization of estrogen, androgen, progestin and glucocorticosteroid in “target tissues” and “non target tissues” in: Receptors and Mechanism of Action of Steroid Hormones. (Pasqualini, J. ed.) New York: Marcel Delker 1976
Stumpf, W.E., Sar, M.: Estrogen target cells in fetal brain. In: Hormones and Brain Development. (Dorner, G., and Kawakami ed.) Amsterdam: M. Elsevier/North-Holland Biomedical Press (1978)
Stumpf, W.E., Narbaitz, R., ad Sar, M.: Estrogen receptors in the fetal mouse. J. Steroid Biochem, in press (1979)
Author information
Authors and Affiliations
Additional information
Supported by PHS Grant #NS 09914
Rights and permissions
About this article
Cite this article
Narbaitz, R., Stumpf, W.E. & Sar, M. Estrogen receptors in mammary gland primordia of fetal mouse. Anat Embryol 158, 161–166 (1980). https://doi.org/10.1007/BF00315903
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00315903