Abstract
By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compatible monokaryons carrying different mtDNA RFLP phenotypes retained mtDNA genotypes from one of the monokaryons, suggesting that mitochondrial inheritance is principally uniparental. In contrast, it was shown that recombinant mtDNA genomes arose in some dikaryons obtained after protoplast cell fusion. Based on these results, a possible mechanism for mitochondrial inheritance in L. edodes is discussed.
Similar content being viewed by others
References
Baptista-Ferreira JLC, Economou A, Casselton LA (1983) Mitochondrial genetics of Coprinus: recombination of mitochondrial genomes. Curr Genet 7:405–407
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Borst P, Grivell LA (1978) The mitochondrial genome of yeast. Cell 15:705–723
Economou A, Lees V, Pukkila PJ, Zolan ME, Casselton LA (1987) Biased inheritance of optional insertions following mitochondrial genome recombination in the basidiomycete fungus Coprinus cinereus. Curr Genet 11:513–519
Fukuda M, Fukumasa-Nakai Y, Hibbett DS, Matsumoto T, Hayashi Y (1994) Mitochondrial DNA restriction fragment polymorphisms in natural populations of Lentinula edodes. Mycol Res 98:169–175
Fukumasa-Nakai Y, Matsumoto T, Fukuda M (1992) Efficient isolation of mitochondrial DNA from higher basidiomycetes for restriction endonuclease analysis. Rep Tottori Mycol Inst (Japan) 30:60–68
Fukumasa-Nakai Y, Matsumoto T, Komatsu M (1994) Fruiting body productivity of protoplast-derived clones in Lentinula edodes. Mycoscience 35:137–139
Hasebe K (1991) Genetic studies on mutants and agronomic characters in Shiitake, Lentinus edodes (in Japanese with English summary). Rep Tottori Mycol Inst (Japan) 29:1–69
Hintz WEA, Anderson JB, Horgan PA (1988) Nuclear migration and mitochondrial inheritance in the mushroom Agaricus bitorquis. Genetics 119:35–41
Komatsu M, Kimura K (1968) Studies on abnormal fruit-bodies of hymenomycetous fungi. V. Fruit-bodies with white pilei of Lentinus edodes (in Japanese with English summary). Rep Tottori Mycol Inst (Japan) 6:9–17
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
May G, Taylor JW (1988) Patterns of mating and mitochondrial DNA inheritance in the agaric basidiomycete Coprinus cinereus. Genetics 118:213–220
Mitchell MB, Mitchell HK (1952) A case of “maternal” inheritance in Neurospora crassa. Proc Natl Acad Sci USA 38: 442–449
Morgan A, Maliga P (1987) Rapid chloroplast segregation and recombination of mitochondrial DNA in Brassica cybrids. Mol Gen Genet 209:240–246
Murakami S, Hasebe K, Tsuneda A (1987) A mutant of Lentinus edodes forming aberrant clamp connections. Rep Tottori Mycol Inst (Japan) 25:49–55
Nagy F, Lazar G, Menczel L, Maliga P (1983) A heteroplasmic state induced by protoplast fusion is a necessary condition of detecting rearrangements in Nicotiana mitochondrial DNA. Theor Appl Genet 66:49–55
Nakai Y (1986) Cytological studies on shiitake, Lentinus edodes (Berk.) Sing. (in Japanese with English summary). Rep Tottori Mycol Inst (Japan) 24:1–102
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fukuda, M., Harada, Y., Imahori, S. et al. Inheritance of mitochondrial DNA in sexual crosses and protoplast cell fusions in Lentinula edodes . Curr Genet 27, 550–554 (1995). https://doi.org/10.1007/BF00314446
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00314446