Abstract
A DNA probe, E9, which has been used extensively in population genetic studies of the barley powdery mildew pathogen, Erysiphe graminis f.sp. hordei, was shown to be homologous to dispersed sequences in the genome of this fungus. In a cross of the isolates CC52 and DH14, fragments with homology to E9 mapped to six clusters of loci. Avirulences matching five resistance genes in barley were controlled by single genes, in accordance with the gene-for-gene hypothesis, while avirulence matching a sixth resistance gene, Mla13, was controlled by two genes. A gene which controls the response to a fungicide, ethirimol, was not linked to any other gene. In all, seven linkage groups, comprising 22 loci, were detected. The results indicate that E9 can be used to identify members of a clone of E.g. f.sp. hordei, but should not be used for quantitative population genetic analysis.
Similar content being viewed by others
References
Borbye L, Linde-Laursen I, Christiansen SK, Giese H (1992) Mycol Res 96: 97–102
Brown JKM, Jørgensen JH (1991) In: Jørgensen JH (ed) Integrated control of cereals mildews: virulence patterns and their change. Risø National Laboratory, Roskilde, pp 263–286
Brown JKM, Wolfe MS (1990) Plant Pathol 39: 376–390
Brown JKM, O'Dell M, Simpson CG, Wolfe MS (1990) Plant Pathol 39: 391–401
Brown JKM, Jessop AC, Rezanoor HN (1991) Proc Roy Soc London B246: 83–90
Brown JKM, Jessop AC, Thomas S, Rezanoor HN (1992) Plant Pathol 41: 126–135
Brown JKM, Simpson CG, Wolfe MS (1993) Plant Pathol 42: 108–115
Christiansen SK, Giese H (1990) Theor Appl Genet 79: 705–712
Flor HH (1956) Adv Genet 8: 29–54
Giese H (1981) Hereditas 95: 51–62
Goodwin SB, Drenth A, Fry WE (1992) Curr Genet 22: 107–115
Hamer JE, Givan S (1990) Mol Gen Genet 223: 487–495
Hollomon DW (1981) Phytopathology 71: 536–540
Jones IT, Davies IJER (1985) Euphytica 34: 499–507
Jørgensen JH (1988) Adv Plant Pathol 6: 137–157
Kohn LM, Stasovski E, Carbone E, Royer J, Anderson JB (1991) Phytopathology 81: 480–485
Kosambi DD (1944) Ann Eugen 13: 172–175
McDonald BA, Martinez JP (1991) Exp Mycol 15: 146–158
Mather K (1938) The measurement of linkage in heredity. Methuen, London
Milgroom MG, Lipari SE, Powell WA (1992) Genetics 131: 297–306
Moseman JG (1963) Phytopathology 53: 1326–1330
Moseman JG, Jørgensen JH (1971) Crop Sci 11: 547–550
Moseman JG, Jørgensen JH (1973) Euphytica 22: 189–196
Moseman JG, Macer RCF, Greeley LW (1965) Trans Br Mycol Soc 48: 479–489
Nei M (1973) Proc Natl Acad Sci USA 70: 3321–3323
O'Dell M, Wolfe MS, Flavell RB, Simpson CG, Summers RW (1989) Plant Pathol 38: 340–351
Rasmussen M, Rossen L, Giese H (1993) Mol Gen Genet 239: 298–303
Smedegård-Petersen V (1967) Roy Vet Agric Univ Yearbook 1967: 1–28
Wiberg A (1974) Hereditas 77: 89–148
Wolfe MS (1984) Plant Pathol 33: 451–466
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brown, J.K.M., Simpson, C.G. Genetic analysis of DNA fingerprints and virulences in Erysiphe graminis f.sp. hordei . Curr Genet 26, 172–178 (1994). https://doi.org/10.1007/BF00313807
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00313807