Abstract
The GGS1/TPS1 gene of the yeast Saccharomyces cerevisiae encodes the trehalose-6-phosphate synthase subunit of the trehalose synthase complex. Mutants defective in GGS1/TPS1 have been isolated repeatedly and they showed variable pleiotropic phenotypes, in particular with respect to trehalose content, ability to grow on fermentable sugars, glucose-induced signaling and sporulation capacity. We have introduced the fdp1, cif1, byp1 and glc6 alleles and the ggs1/tps1 deletion into three different wild-type strains, M5, SP1 and W303-1A. This set of strains will aid further studies on the molecular basis of the complex pleiotropic phenotypes of ggs1/tps1 mutants. The phenotypes conferred by specific alleles were clearly dependent on the genetic background and also differed for some of the alleles. Our results show that the lethality caused by single gene deletion in one genetic background can become undetectable in another background. The sporulation defect of ggs1/tps1 diploids was neither due to a deficiency in G1 arrest, nor to the inability to accumulate trehalose. Ggs1/tps1 Δ mutants were very sensitive to glucose and fructose, even in the presence of a 100-fold higher galactose concentration. Fifty-percent inhibition occurred at concentrations similar to the Km values of glucose and fructose transport. The inhibitory effect of glucose in the presence of a large excess of galactose argues against an overactive glycolytic flux as the cause of the growth defect. Deletion of genes of the glucose carrier family shifted the 50% growth inhibition to higher sugar concentrations. This finding allows for a novel approach to estimate the relevance of the many putative glucose carrier genes in S. cerevisiae. We also show that the GGS1/TPS1 gene product is not only required for the transition from respirative to fermentative metabolism but continuously during logarithmic growth on glucose, in spite of the absence of trehalose under such conditions.
Similar content being viewed by others
References
Bañuelos M, Fraenkel DG (1982) Mol Cell Biol 2:921–929
Bell W, Klaassen P, Ohnacker M, Boller T, Herweijer M, Schoppink P, Van der Zee P, Wiemken A (1992) Eur J Biochem 209:951–959
Berben G, Dumont J, Gilliquet V, Bolle P-A, Hilger F (1991) Yeast 7:475–477
Bisson LF, Fraenkel DG (1983) Proc Natl Acad Sci USA 80:1730–1734
Bisson LF, Neigeborn L, Carlson M, Fraenkel DG (1987) J Bacteriol 169:1656–1662
Bisson LF, Coons DM, Kruckeberg AI, Lewis DA (1993) Crit Rev Biochem Mol Biol 28:259–308
Blázquez MA, Gancedo C (1994) Curr Genet 25:89–94
Blázquez MA, Lagunas R, Gancedo C, Gancedo JM (1993) FEBS Lett 329:51–54
Breitenbach-Schmitt I, Schmitt HD, Heinisch J, Zimmermann FK (1984) Mol Gen Genet 195:536–540
Cannon JF, Pringle JR, Fiechter A, Khalil M (1994) Genetics 136: 485–503
Celenza JL, Marshall-Carlson L, Carlson M (1988) Proc Natl Acad Sci USA 85:2130–2134
Charlab R, Oliveira DE, Panek AD (1985) Braz J Med Biol Res 18: 447–454
De Virgilio C, Simmen U, Hottiger T, Boller T, Wiemken A (1990) FEBS Lett 273:107–110
De Virgilio C, Bürckert N, Bell W, Jenö P, Boller T, Wiemken A (1993) Eur J Biochem 212:315–323
François J, Villanueva ME, Hers HG (1988) Eur J Biochem 174:551–559
François J, Neves MJ, Hers HG (1991) Yeast 7:575–587
Gancedo JM, Gancedo C (1971) Arch Microbiol 76:132–138
Gietz RD, Sugino A (1988) Gene 74:527–534
González MI, Stucka R, Blázquez MA, Feldmann H, Gancedo C (1992) Yeast 8:183–192
Herskowitz I, Jensen RE (1991) Methods Enzymol 194:132–146
Hohmann S, Huse K, Valentin E, Mbonyi K, Thevelein JM, Zimmermann FK (1992) J Bacteriol 174:4183–4188
Hohmann S, Neves MJ, de Koning W, Alijo R, Ramos J, Thevelein JM (1993) Curr Genet 23:281–289
Hohmann S, Van Dijck P, Luyten K, Thevelein JM (1994) Curr Genet (in press)
Kane SM, Roth RM (1974) J Bacteriol 118:8–14
Ko CH, Liang H, Gaber RF (1993) Mol Cell Biol 13:638–648
Kruckeberg AL, Bisson LF (1990) Mol Cell Biol 10:5903–5913
Kuo SC, Christensen MS, Cirillo VP (1970) J Bacteriol 103:671–678
Lewis DA, Bisson LF (1991) Mol Cell Biol 11:3804–3813
Manning AM, Rosenblom CL, Beaudet AL (1992) EMBL accession number MM88172
McDougall J, Kaasen I, Strøm AR (1993) FEMS Microbiol Lett 107:25–30
Murray AW, Szostak JW (1983) Cell 34:961–970
Navon G, Shulman RG, Yamane T, Eccleshal TR, Lam K-B, Baronofsky JJ, Marmur J (1979) Biochemistry 18:4487–4499
Neves MJ, Jorge JA, François JM, Terenzi HF (1991) FEBS Lett 283: 19–22
Poll KW van de, Schamhart DJH (1977) Mol Gen Genet 154:61–66
Poll KW van de, Kerkenaar A, Schamhart DHJ (1974) J Bacteriol 117:965–970
Popolo L, Vanoni M, Alberghina L (1982) Exp Cell Res 142:69–78
Prior C, Fukuhara H, Blaisonneau J, Wesolowski-Louvel M (1993) Yeast 9:1373–1377
Ramos J, Szkutnicka K, Cirillo VP (1988) J Bacteriol 170:5375–5377
Roth R (1970) J Bacteriol 101:53–57
Rothstein RJ (1983) Methods Enzymol 101:202–211
Schaaff I, Green JBA, Gozalbo D, Hohmann S (1989) Curr Genet 15:75–81
Sherman F, Fink GR, Hicks JB (1986) Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sols A (1976) The Pasteur effect in the allosteric era. In: Kornberg A et al. (eds) Reflections in biochemistry. Pergamon Press, Oxford, pp 199–206
Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76:1035–1039
Sur IP, Lobo Z, Maitra PK (1994) Yeast 10:199–209
Thevelein JM (1988) Exp Mycol 12:1–12
Thevelein JM (1992) Ant van Leeuwenhoek 62:109–130
Thomas BJ, Rothstein R (1989) Cell 56:619–630
Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S, Broach J, Matsumoto K, Wigler M (1995) Cell 40:27–36
Van Aelst L, Hohmann S, Zimmermann FK, Jans AWH, Thevelein JM (1991) EMBO J 10:2095–2104
Van Aelst L, Hohmann S, Bulaya B, de Koning W, Sierkstra L, Neves MJ, Luyten K, Alijo R, Ramos J, Coccetti P, Martegani E, de Magalhães-Rocha NM, Brandão RL, Van Dijck P, Vanhalewyn M, Durnez P, Jans AWH, Thevelein JM (1993) Mol Microbiol 8: 927–943
Vandercammen A, François J, Hers H-G (1989) Eur J Biochem 182: 613–620
Vuorio OE, Kalkkinen N, Londesborough J (1993) Eur J Biochem 216:849–861
Zakian VA, Kupfer DM (1982) Plasmid 8:15–28
Zamenhoff S (1957) Methods Enzymol 3:696–704
Author information
Authors and Affiliations
Additional information
Communicated by F. K. Zimmermann
Rights and permissions
About this article
Cite this article
Neves, M.J., Hohmann, S., Bell, W. et al. Control of glucose influx into glycolysis and pleiotropic effects studied in different isogenic sets of Saccharomyces cerevisiae mutants in trehalose biosynthesis. Curr Genet 27, 110–122 (1995). https://doi.org/10.1007/BF00313424
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00313424