Summary
Overlapping restriction fragments of chloroplast DNA from the conifer Pinus contorta were cloned. Out of a total of 49 clones, 33 comprise the minimum set required to represent the entire genome. Using the purified inserts of these clones as probes in filter hybridizations, all sites for the three restriction enzymes KpnI, HapI and SacI in the P. contorta chloroplast genome were mapped. Heterologous filter hybridizations and sequence analysis of some of the P. contorta clones were used to determine the position of 15 genes on the restriction map. The size of the genome, which lacks an inverted repeat organization, was found to be approximately 121 kilobase pairs (kbp). Unusual features of this genome are a duplication of the psbA gene and the presence of two genes, gidA and frxC, which are not found in angiosperms. The genome appeared essentially colinear with that of Pinus radiata, for which a map has previously been published. Two different restriction fragment length polymorphisms were found to be produced by variable numbers of copies of 124 bp-and 150 bp-long, tandemly repeated elements.
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References
Alt J, Morris J, Westhoff P, Herrmann RG (1984) Curr Genet 8:597–606
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heynecker HL, Boyer HW, Crosa JH, Falkow S (1977) Gene 2:95–113
Bonham-Smith PC, Bourque DP (1989) Nucleic Acids Res 17:2057–2080
Dron M, Rahire M, Rochaix J-D (1982) J Mol Biol 162:775–793
Goldschmidt-Clermont M, Choquet Y, Girard-Bascou J, Michel F, Schirmer-Rahire M, Rochaix J-D (1991) Cell 65:135–143
Hanley-Bowdoin L, Chua N-H (1987) Trends Biochem Sci 12:67–70
Herrmann RG, Alt J, Schiller B, Widger WR, Cramer WA (1984) FEBS Lett 176:239–244
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C-R, Meng B-Y, Li Y-Q, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Knoll AH, Rothwell GW (1981) Paleobiology 7:7–35
Lidholm J, Szmidt AE, Hällgren J-E, Gustafsson P (1988) Mol Gen Genet 212:6–10
Lidholm J, Szmidt AE, Gustafsson P (1991) Mol Gen Genet (in press)
Morris J, Herrmann RG (1984) Nucleic Acids Res 12:2837–2850
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574
Ohyama K, Kohchi T, Sano T, Yamada Y (1988) Trends Biochem Sci 13:19–22
Palmer JD (1985) Annu Rev Genet 19:325–354
Palmer JD, Thompson WF (1982) Cell 29:537–550
Palmer JD, Jansen RK, Michaels HJ, Chase MW, Manhart JR (1988) Ann Missouri Bot Gard 75:1180–1206
Rochaix JD (1978) J Mol Biol 126:597–617
Ruf M, Kössel H (1988) FEBS Lett 240:41–44
Shinozaki K, Ohme M, Tanaka M, Wakazugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Strauss SH, Palmer JD, Howe GT, Doerksen AH (1988) Proc Natl Acad Sci USA 85:3898–3902
Szmidt AE, Lidholm J, Hällgren J-E (1986) In: Lindgren D (ed) Provenances and forest tree breeding for high latitudes. Proceedings of the Frans Kempe symposium, Umeå, pp 269–280
Szmidt AE, El-Kassaby YA, Sigurgeirsson A, Aldén T, Lindgren D, Hällgren J-E (1988) Theor Appl Genet 76:841–845
Takeshita S, Sato M, Toba M, Masahashi W, Hashimoto-Gotoh T (1987) Gene 61:63–74
Tomioka N, Shinozaki K, Sugiura M (1981) Mol Gen Genet 184:359–363
Umesono, K, Ozeki H (1987) Trends Genet 3:281–287
Wagner DB, Furnier GR, Saghai-Marof MA, Williams SM, Dancik BP, Allard RW (1987) Proc Natl Acad Sci USA 84:2097–2100
White EE (1990) Theor Appl Genet 79:119–124
Vieira J, Messing J (1982) Gene 19:259–268
Wolfe KH, Sharp PM (1988) Gene 66:215–222
Wolfe KH, Gouy M, Yang Y-W, Sharp PM, Li W-H (1989) Proc Natl Acad Sci USA 86:6201–6205
Yanisch-Perron C, Vieira J, Messing J (1985) Gene 33:103–119
Zurawski G, Clegg MT (1987) Annu Rev Plant Physiol 38:391–418
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Lidholm, J., Gustafsson, P. The chloroplast genome of the gymnosperm Pinus contorta: a physical map and a complete collection of overlapping clones. Curr Genet 20, 161–166 (1991). https://doi.org/10.1007/BF00312780
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DOI: https://doi.org/10.1007/BF00312780