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Catecholamine-containing, serotonin-like and neuropeptide FMRFamide-like immunoreactive cells and processes in the nervous system of the pilidium larva (Nemertini)

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Summary

Pilidium larvae at different developmental stages have been investigated for the occurrence of glyoxylic acid induced fluorescence in catecholamines (CA), and serotonin-like (5-HT) and neuropeptide FMRFamide-like (FMRFamide) immunoreactivity (ir). The distribution of CA, 5-HT-ir and FMRFamide-ir cells and processes was compared with the location of nerve processes as found by transmission electron microscopy (TEM). In the pilidium larvae the marginal and oral nerves contain CA and 5-HT-ir processes and 5-HT-ir unipolar cells. The posterior suboral nerve contain CA and 5-HT-ir processes, whereas in the anterior suboral nerve neither CA nor 5-HT-ir and FMRFamide-ir were observed. The lateral helmet nerve contains FMRFamide-ir processes and unipolar cells. In the epidermis CA and 5-HT-ir multipolar cells were found. The juvenile “worm” that develops inside the pilidium larva was found to contain only 5-HT-ir. A pair of lateral cords extent the whole length of the juvenile and anteriorly they form the anterior ventral cerebral commissure. Also, from the anterior part of the lateral cords projects a pair of circumrhynchodeal processes which dorsally form the dorsal cerebral commissure. A pair of proboscis processes originate from the circumrhynchodeal processes and extend the whole length of the probosics. From the dorsal cerebral commissure cephalic processes project rostrally and ventrally. Only unipolar 5-HT-ir cells were observed, and they were located along the lateral cords into which their processes extend.

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Abbreviations

AEC :

3-amino-9-ethylcarbazole

ap :

apical plate

arp :

anterior accessory ridge processes

ason :

anterior suboral nerve

CA :

catecholamines

cd :

cephalic discs

cp :

cephalic processes

crp :

circumrhynchodeal processes

DAB :

3,3'-diaminobenzidine

dc :

dorsal cerebral commissure

epi :

epidermis

es :

oesophagus

fl :

fore lobe

FMRFamide :

phe—met—arg—phe—NH2

Go :

goat

GS :

goat serum

hl :

hind lobe

int :

intestine of the juvenile

lhn :

lateral helmet nerve

lhp :

lateral helmet processes

ll :

lateral lobe

lp :

lateral processes of the juvenile

mcb :

marginal ciliary band

me :

mesoderm

mn :

marginal nerve

moc :

monociliary cell

mp :

marginal processes

mu :

muscle

muc :

multiciliary cell

n 1, n 2, n 3 :

division of marginal nerve

on :

oral nerve

op :

oral processes

pb :

proboscis

pp :

proboscis processes

pson :

posterior suboral nerve

psop :

posterior suboral processes

Ra :

rabbit

sd :

stomodeum

st :

stomach

td :

trunk discs

tr :

trunk

TRITC :

tetramethylrhodamine isothiocyanate

vc :

ventral cerebral commissure

z 1, z 2 :

ciliary zones of marginal ciliary band

5-HT :

serotonin

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  1. Institute of Cell Biology and Anatomy, University of Copenhagen, Universitetsparken 15, DK-2100, Copenhagen Ø, Denmark

    Anders Hay-Schmidt

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Hay-Schmidt, A. Catecholamine-containing, serotonin-like and neuropeptide FMRFamide-like immunoreactive cells and processes in the nervous system of the pilidium larva (Nemertini). Zoomorphology 109, 231–244 (1990). https://doi.org/10.1007/BF00312190

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