Abstract
The cytokinetics of the human pancreatic adenocarcinoma cell line Capan-2 were analyzed by a bromodeoxyuridine (BrdUrd)/DNA analysis with flow cytometry (FCM). The reproducibility of data was shown to be superior to that achieved using a DNA analysis program with FCM. The perturbation of cytokinetics following irradiation was examined. Bivariate distribution was able to distinguish each cell phase cohort, even following as strong an irradiation as 10 Gy, a condition for which DNA analysis proved unusable. BrdUrd/DNA analysis showed the G2M phase population after irradiation with 2 Gy to be significantly greater (P<0.005) than that of the untreated cells. This difference was not detectable by the DNA analysis program due to the large standard deviation (SD) of the data. BrdUrd/DNA analysis has a sufficient quantitative accuracy that strongly perturbed cytokinetics in patients treated with various therapeutic agents, for example, with combined therapies, can thus be analyzed by this approach.
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References
Simpson-Herren L, Sanford AH, Holmquist JP, Springer TA, Lloyd HH (1976) Ambiguity of the thymidine index. Cancer Res 36:4705–4709
Baisch H, Beck HP, Christensen IJ, et al (1982) A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry. Cell Tissue Kinet 15:235–249
Gratzner HG, Leif RC (1981) An immunofluorescence method for monitoring DNA synthesis by flow cytometry. Cytometry 1:385–389
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW (1983) Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA 80:5573–5577
Dean PN, Dolbeare F, Gratzner H, Rice GC, Gray JW (1984) Cell-cycle analysis using a monoclonal antibody to BrdUrd. Cell Tissue Kinet 17:427–436
Pallavicini MG, Summers LJ, Dolbeare FD, Gray JW (1985) Cytokinetic properties of asynchronous and cytosine arabinoside perturbed murine tumors measured by simultaneous bromodeoxyuridine/DNA analyses. Cytometry 6:602–610
Sasaki K, Murakami T, Ogino T, Takahashi M, Kawasaki S (1986) Flow cytometric estimation of cell cycle parameters using a monoclonal antibody to bromodeoxyuridine. Cytometry 7:391–395
Tsurusawa M, Niwa M, Katano N, Fujimoto T (1988) Flow cytometric analysis by bromodeoxyuridine/DNA assay of cell cycle perturbation of methotrexate-treated mouse L1210 leukemia cells. Cancer Res 48:4288–4293
Tsurusawa M, Niwa M, Katano N, Fujimoto T (1990) Methotrexate cytotoxicity as related to irreversible S phase arrest in mouse L1210 leukemia cells. Jpn J Cancer Res 81:85–90
Dean PN, Jett JH (1974) Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol 60:523–527
Dean PN (1980) A simplified method of DNA distribution analysis. Cell Tissue Kinet 13:299–308
Fogh J, Fogh JM, Orfeo T (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59:221–225
Tobey RA, Crissman HA (1972) Use of flow microfluorometry in detailed analysis of effects of chemical agents on cell cycle progression. Cancer Res 32:2726–2732
Yataganas X, Clarkson BD (1974) Flow microfluorometric analysis of cell killing with cytotoxic drugs. J Histochem Cytochem 22:651–659
Krishan A, Pitman SW, Tattersall MHN, Paika KD, Smith DC, Frei E III (1976) Flow microfluorometric patterns of human bone marrow and tumor cells in response to cancer chemotherapy. Cancer Res 36:3813–3820
Fried J (1976) Method for the quantitative evaluation of data from flow microfluorometry. Comp Biomed Res 9:263–276
Kal HB, Hatfield M, Hahn GM (1975) Cell cycle progression of murine sarcoma cells after X-irradiation or heat shock. Radiology 117:215–217
Pallavicini MG, Lalande ME, Miller RG, Hill RP (1979) Cell cycle distribution of chronically hypoxic cells and determination of the clonogenic potential of cells accumulated in G2+M phases after irradiation of a solid tumor in vivo. Cancer Res 39:1891–1897
Cao S, Skog S, Tribukait B (1982) Growth kinetics of Bp8 mouse ascites sarcoma after single dose whole body irradiation. I. Analysis of the relative and total numbers of cells in various parts of the cell cycle. Acta Radiol Oncol 21:255–265
Dressler LG, Seamer L, Owens MA, Clark GM, McGuire WL (1987) Evaluation of a modeling system for S-phase estimation in breast cancer by flow cytometry. Cancer Res 47:5294–5302
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Nakata, B., Chung, YS. & Sowa, M. A flow cytometric bromodeoxyuridine/DNA analysis for cytokinetics of the pancreatic cancer cell line capan-2 after irradiation. Surg Today 23, 717–721 (1993). https://doi.org/10.1007/BF00311711
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DOI: https://doi.org/10.1007/BF00311711