Abstract
The presence of high affinity binding sites for 3H-imipramine (3H-IMI) in human platelets is by now well established. This recognition site is associated with the transporter for 5HT, and may be a biological marker in depression. Fluctuations of other putative biological markers of depression (i.e. platelet MAO activity) have been demonstrated and shown to be correlated with variations in steroid hormones. Therefore, the KD and Bmax of 3H-IMI binding was determined in platelets of young women during the menstrual cycle. Our results indicate that within the limits of intraindividual variations, neither the KD or the Bmax of 3H-IMI binding in platelets is significantly modified during the menstrual cycle.
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References
Abbot R, Chandra M, Chandra R (1983) Total platelet count, adhesive platelet count, platelet adhesiveness in normal menstrual cycle and functional uterine bleedings: a comparative study. Asia-Oceania J Obstet Gynaecol 913:265–269
Asberg M, Bertilsson L, Tuck D, Cronholm B, Sjöqvist F (1973) Indoleamine metabolites in the cerebrospinal fluid of depressed patients before and during treatment with nortriptyline. Clin Pharmacol Ther 14:277–286
Baron M, Kowalik S, Barkai A, Gruen R (1984) High affinity 3H-imipramine binding in human platelets: age and sex effects. Neuropsychobiology 11:178–180
Belmaker RH, Murphy DL, Wyatt JR, Loriaux DL (1974) Human platelet monoamine oxidase changes during the menstrual cycle. Arch Gen Psychiatry 31:553–556
Briley MS, Langer SZ, Raisman R, Sechter D, Zarifian E (1980) Tritiated imipramine binding sites are decreased in platelets of untreated depressed patients. Science 209:303–305
Castren E, Tuomisto J (1982) Influence of the hormonal balance of late pregnancy on the active uptake of 5-hydroxytryptamine by human blood platelets. Br J Pharmacol 77:436P
Gonzales GF (1980) Blood levels of 5-hydroxytryptamine in human beings under several physiological situations. Life Sci 27:647–650
Guicheney P, Marcel D (1985) Decrease of platelet serotonin content in ovariectomized female rats. Endocrinology (in press)
Harrison KL, Bramich L, Collins KA (1982) Platelet count during normal pregnancy. Aust NZ J Obstet Gynaecol 22:74–75
Katona CLE, Theodorou AE, Missouris CG, Bourke MP, Chamberlain GVP, Horton RW, Paykel ES, Kelly S (1984) Platelet 3H-imipramine binding and mood in pregnancy and the puerperium. Abstracts 14th C.I.N.P. Congress, Florence, Italy, p 202
Karpatkin S (1978) Heterogeneity of human platelets VI Correlation of platelet function with platelet volume. Blood 51:307–316
Langer SZ, Moret C, Raisman R, Dubocovich ML, Briley M (1980a) High affinity 3H-imipramine binding in rat hypothalamus is associated with the uptake of serotonin but not norepinephrine. Science 210:1133–1135
Langer SZ, Briley MS, Raisman R, Henry JF, Morselli PL (1980b) Specific 3H-imipramine binding in human platelets: influence of sex and age. Naunyn-Schmiedeberg's Arch Pharmacol 313:189–194
Langer SZ, Zarifian E, Briley M, Raisman R, Sechter D (1981) High-affinity binding of [3H]-imipramine in brain and platelets and its relevance to the biochemistry of affective disorders. Life Sci 29:211–220
Lewis DA, Mc Chesney C (1985) Tritiated imipramine binding distinguishes among subtypes of depression. Arch Gen Psychiatry 42:485–488
Mellerup ET, Plenge P, Rosenberg R (1982) 3H-imipramine binding sites in platelets from psychiatric patients. Psychiatr Res 7:221–227
Moos RH (1968) The development of a menstrual distress questionnaire. Psychosom Med 30:853–867
Paul SM, Rehavi M, Rice K (1981) Does high affinity [3H]imipramine binding label serotonin reuptake sites in brain and platelets? Life Sci 28:2253–2260
Poirier MF, Loo H, Scatton B, Le Fur G (1985) Platelet monoamine oxidase activity and dihydroxyphenyl glycol during the menstrual cycle. Neuropsychobiology (in press)
Raisman R, Sechter D, Briley MS, Zarifian E, Langer SZ (1981) High affinity 3H-imipramine binding in platelets from untreated and treated depressed patients compared to healthy volunteers. Psychopharmacology 75:368–371
Raisman R, Briley MS, Bouchami F, Sechter D, Zarifian E, Langer SZ (1982) 3H-Imipramine binding and serotonin uptake in platelets from untreated depressed patients and control volunteers. Psychopharmacology 77:332–335
Ritvo E, Yawiler A, Geller E, Plotkin S, Mason A, Saeger K (1971) Maturational changes in blood serotonin levels and platelet counts. Biochem Med 5:90–96
Robinson DS, Nies A (1980) Demographic, biologic and other variables affecting monoamine oxidase activity. Schizophr Bull 6:298–307
Schulman NR, Watkins SP Jr, Itscoitz SB (1968) Evidence that the spleen retain the youngest and hemostatically most effective platelets. Trans Assoc Am Physicians 81:302–313
Thomson J, Maddock J, Aylamard M, Oswald I (1977) Relationship between nocturnal plasma oestrogen concentration and free plasma tryptophan in perimenopausal women. J Endocrinol 72:395–397
Wirz-Justice A, Pühringer W, Hole G, Menzi R (1975) Monoamine oxidase and free tryptophan in human plasma: normal variations and their implications for biochemical research in affective disorders. Pharmacopsychiatry 8:310–317
Wirz-Justice A, Chappuis-Arnodt E (1976) Sex specific differences in chlorimipramine inhibition of serotonin uptake in human platelets. Eur J Pharmacol 40:21–25
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Poirier, MF., Benkelfat, C., Galzin, AM. et al. Platelet 3H-imipramine binding and steroid hormones serum concentrations during the menstrual cycle. Psychopharmacologia 88, 86–89 (1986). https://doi.org/10.1007/BF00310518
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DOI: https://doi.org/10.1007/BF00310518