Structure and expression characteristics of the chloroplast DNA region containing the split gene for tRNA^Gly (UCC) from mustard (Sinapis alba L.)

Abstract

The mustard chloroplast gene trnG-UCC is split by a 717-bp group-II intron. Northern hybridization and RNase protection experiments suggest cotranscription with the upstream psbK-psbI operon, but not with the downstream trnR-UCU gene. The ends of most RNase-protected fragments between psbI and trnG correlate with the position of two potential stem-loop structures in this region, which could act as RNA processing elements. However, one RNA 5′ end, approximately 75 bp upstream of the trnG 5′ exon, does not so correlate and is preceded by prokaryotic-type ‘-10’ and ‘-35’ sequence elements. This suggests the possibility that a fraction of the trnG transcripts is initiated here. All precursor transcripts spanning the trnG region seem to have a common 3′ end, which was located 117 bp downstream from the 3′ exon, immediately after a stem-loop region. During seedling development, the major 0.8–0.9-kb trnG precursor transcripts show a transient maximum level at around 48 h after sowing, at a time when the mature tRNA begins to accumulate to constant levels. No significant differences in transcript patterns were observed either in the light or in darkness.