Summary
Fractionation of intact chromosomes of the ascomycete Podospora anserina by contour-clamped homogenous electric field (CHEF) gel electrophoresis resulted in the resolution of five distinct chromosomal bands. Two of these bands migrated as doublets. Using chromosomal standards from Schizosaccharomyces pombe we estimated the size of the P. anserina genome to about 33.4 megabases (Mb). By heterologous hybridization of fractionated chromosomes the rDNA locus was identified on one of the two chromosomes migrating at about 4.9 Mb.
This is a preview of subscription content, log in via an institution to check access.
References
Brody H, Carbon J (1989) Proc Natl Acad Sci USA 86: 6260–6263
Esser K (1974) In: King RC (ed) Handbook of genetics, vol I. Plenum Press, New York, pp 531–551
Esser K (1985) In: Bergener M, Ermini M, Stähelin HB (eds) The 1984 Sandoz Lectures in Gerontology. Thresholds in aging. Academic Press, London, pp 3–20
Franke G (1962) Z Vererbungsl 93: 109–117
Osiewacz HD (1990) Mutat Res 237: 1–8
Orbach MJ, Vollrath D, Davis RW, Yanofsky C (1988) Mol Cell Biol 8: 1469–1473
Skatrud PL, Queener SW (1989) Gene 78: 331–338
Verbeet MP, Kloodwijk J, van Heerrikhuizen H, Fontijin R, Vreugdenhil E, Plana RJ (1983) Gene 23: 53–63
Author information
Authors and Affiliations
Additional information
Communicated by K. Wolf
Rights and permissions
About this article
Cite this article
Osiewacz, H.D., Clairmont, A. & Huth, M. Electrophoretic karyotype of the ascomycete Podospora anserina . Curr Genet 18, 481–483 (1990). https://doi.org/10.1007/BF00309921
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00309921