Abstract
Humic compounds with β-glucosidase activity were extracted from soil using tetrasodium pyrophosphate as extracting solution. Mixture of soil samples with 0.01 M pyrophosphate in a ratio of 1:5 (w/v), adjustment to pH 7.0–7.3, extraction for 18 h with reciprocating agitation, and bacteriological filtration after centrifugation were the optimum conditions for extraction of the β-glucosidase complexes. Otherwise, experimental conditions for extraction indicated that the concentration and pH of pyrophosphate were the factors with the most influence on enzymatic extraction yields. The results indicated that the β-glucosidase was extracellular and associated with soil humates.
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Busto, M.D., Perez-Mateos, M. Extraction of humic-β-glucosidase fractions from soil. Biol Fert Soils 20, 77–82 (1995). https://doi.org/10.1007/BF00307845
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DOI: https://doi.org/10.1007/BF00307845