Summary
The axonal flow of neurosecretory elementary granules has been studied in the paraventricular neurons of the rat (PVN), with the help of three techniques: light microscopy, radioautography after labelling with 35S-L-cysteine, and electron microscopy.
Colchicine treatment does not alter the uptake of 35S cysteine in the PVN but the flow of labelled neurosecretory material towards the neurohypophysis is interrupted. Interruption of the axonal flow is also evidenced by the stagnation of neurosecretory granules at the periphery of the neuronal cytoplasm and by the presence of numerous axonal swellings, heavily loaded with neurosecretory granules and often containing abnormal elongated granules, surrounded by a single membrane, oriented more or less parallely to the long axis of the axons. Other cell organelles and neurotubules are not altered. The present experiments bring further evidence of the arrest by colchicine of the axonal flow of secretory granules without apparent changes of the neurotubules.
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Adelman, M. R., Borisy, G. G., Shelanski, M. L., Weisenberg, R. C., Taylor, E. W.: Cytoplasmic filaments and tubules. Fed. Proc. 27, 1186–1193 (1968).
Bajer, A.: Notes on ultrastructure and some properties of transport within the living mitotic spindle. J. Cell Biol. 33, 713–719 (1967).
Behnke, O., Forer, A.: Evidence for four classes of microtubules in individual cells. J. Cell Sci. 2, 169–192 (1967).
Borisy, G. G., Taylor, E. W.: The mechanism of action of colchicine. Binding of colchicine-3H to cellular protein. J. Cell Biol. 34, 525–534 (1967).
Borisy, G. G., Taylor, E. W.: Colchicine binding to sea urchin eggs and the mitotic apparatus. J. Cell Biol. 34, 535–548 (1967).
Bunge, R., Bunge, M.: Electron microscopic observations on colchicine-induced changes in neuronal cytoplasm. Anat. Rec. 160, 323 (1968).
Bunge, R., Bunge, M.: A comparison of neuronal changes following colchicine treatment with observations on other conditions involving the accumulation of neurofilaments. J. Neuropath. exp. Neurol. 28, 169 (1969).
Dahlström, A.: Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat. Europ. J. Pharmacol. 5, 111–113 (1968).
Dahlström, A., Häggendal, J.: Some quantitative studies on the noradrenaline content in the cell bodies and terminals of a sympathetic adrenergic neuron system. Acta physiol. scand. 67, 271–277 (1966).
Dahlström, A., Häggendal, J.: Studies on the transport and life-span of amine storage granules in a peripheral adrenergic neuron system. Acta physiol. scand. 67, 278–288 (1966).
Dustin, P., Jr.: Microtubules et microfilaments: leur rôle dans la dynamique cellulaire. Arch. Biol. (Liège) (sous presse).
Feit, H., Barondes, S. H.: Colchicine-binding activity in particulate fractions of mouse brain. J. Neurochem. 17, 1355–1364 (1970).
Feit, H., Dutton, G., Barondes, S. B., Shelanski, M. L.: Metabolism of microtubule protein in mouse brain. J. Cell Biol. 47, 60a (1970).
Fernandez, H. L., Burton, P. R., Samson, F. E.: Axoplasmic transport in the crayfish nerve cord. The role of fibrillar constituents of neurons. J. Cell Biol. 51, 176–192 (1971).
Flament-Durand, J.: Etude des relations hypothalamohypophysaires à l'aide de radioisotopes marqués au soufre 35. C. R. Acad. Sci. (Paris) 252, 3487–3500 (1961).
Flament-Durand, J.: Contribution a l'étude de la neurosécrétion chez le rat par la méthode autoradiographique. In: Neurosécrétion (F. Stutinsky, ed.), p. 51–76. Berlin-Heidelberg-New York: Springer 1967.
Flament-Durand, J.: Ultrastructural aspects of the paraventricular nuclei in the rat. Z. Zellforsch. 116, 61–69 (1971).
Fulton, C., Kane, R. E., Stephens, R. E.: Serological similarity of flagellar and mitotic microtubules. J. Cell Biol. 50, 762–773 (1971).
Hökfelt, T., Dahlström, A.: Effects of two mitosis inhibitors (colchicine and vinblastine) on the distribution and axonal transport of noradrenaline storage particles, studied by fluorescence and electron microscope. Z. Zellforsch. 119, 460–482 (1971).
Huneeus, F. C., Davison, P. F.: Fibrillar proteins from squid axons. I. Neurofilament protein. J. molec. Biol. 52, 415–428 (1970).
Karlsson, J.-O., Hansson, H.-A., Sjöstrand, J.: Effect of colchicine on axonal transport and morphology of retinal ganglion cells. Z. Zellforsch. 115, 265–283 (1971).
Kirkpatrick, J. B., Hyams, L., Thomas, V. L., Howley, P. M.: Purification of intact microtubules from brain. J. Cell Biol. 47, 384–394 (1970).
Klein, M. J., Porte, A., Stutinsky, F.: Comparaison ultrastructurale des noyaux neurosécrétoires hypothalamiques chez le rat normal ou en état de surcharge. Bull. Ass. Anat. (Nancy) 142, 1066–1072 (1968).
Kreutzberg, G. W.: Neuronal dynamics and axonal flow. IV. Blockage of intra-axonal enzyme transport by colchicine. Proc. nat. Acat. Sci. (Wash.) 62, 722–728 (1969).
McIntosh, J. R., Hepler, P. K., Van Wie, D. G.: Model for mitosis. Nature (Lond.) 224, 659–663 (1969).
Norström, A., Hansson, H.-A., Sjöstrand, J.: Effects of cholchicine on axonal transport and ultrastructure of the hypothalamo-neurohypophyseal system of the rat. Z. Zellforsch. 113, 271–293 (1971).
Ochs, S.: The dependence of fast transport in mammalian nerve fibers on metabolism. Acta neuropath. Suppl. (Berl.) 5, 86–96 (1971).
Ochs, S., Ranish, N.: Metabolic dependence of fast axoplasmic transport in nerve. Science 167, 878–879 (1970).
Peterson, E. R.: Neurofibrillar alterations in cord-ganglion cultures exposed to spindle inhibitors. J. Neuropath. exp. Neurol. 28, 168 (1969).
Pilgrim, C.: Morphologische und funktionelle Untersuchungen zur Neurosekretbildung. Ergebn. Anat. Entwickl.-Gesch. 41, 1–77 (1969).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 7, 280–213 (1963).
Schmitt, F. O.: Fibrous proteins-neuronal organelles. Proc. nat. Acad. Sci. (Wash.) 60, 1092–1101 (1969).
Shelanski, M. L., Taylor, E. W.: Isolation of a protein sub-unit from microtubules. J. Cell Biol. 34, 549–554 (1967).
Sjöstrand, J., Frizell, M., Hasselgren, P.-O.: Effects of colchicine on axonal transport in peripheral nerves. J. Neurochem. 17, 1563–1570 (1970).
Sloper, J. C.: The application of newer histochemical and isotope techniques for the localisation of protein-bound cystine or cysteine to the study of hypothalamic neurosecretin in normal and pathological conditions. II Int. Symp. Neurosekretion, eds. W. Bargmann, B. Hanström, E. Scharrer, p. 20. Berlin-Göttingen-Heidelberg: Springer 1958.
Sloper, J. C., Arnott, D. J., King, B. C.: Sulphur metabolism in the pituitary and hypothalamus of the rat: a study of radioisotope uptake after injection of S 35 DL-cysteine, methionine and sodium sulphate. J. Endocr. 20, 9–23 (1960).
Weisenberg, R. C., Borisy, G. G., Taylor, E. W.: The colchicine-binding protein of mammalian brain and its relation to microtubules. Biochemistry 7, 4466–4478 (1968).
Wiśniewski, H., Shelanski, M. L., Terry, R. D.: Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells. J. Cell Biol. 38, 224–229 (1968).
Wiśniewski, H., Terry, R. D., Shelanski, M. L.: Neurofibrillary degeneration of nerve cells after subarachnoid injection of mitotic spindle inhibitors. J. Neuropath. exp. Neurol. 28, 168–169 (1969).
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This work was supported by a grant (1970/1971) from the Belgian National Fund for Scientific Research (J. F.-D), and by grant No 1120 from the Belgian National Fund for Medical Research (P. D).
The authors wish to thank Mrs. A.-M. Hunninck-Couck for her devoted and skillful technical assistance, and are endebted to Dr. J. C. Heuson for kindly supplying the rats.
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Flament-Durand, J., Dustin, P. Studies on the transport of secretory granules in the magnocellular hypothalamic neurons. Z.Zellforsch 130, 440–454 (1972). https://doi.org/10.1007/BF00306998
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DOI: https://doi.org/10.1007/BF00306998