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Histochemical observations of protein-bound amino groups in human developing deciduous teeth

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Summary

Human developing deciduous teeth were studied by histochemical methods for protein groups; dinitrofluorobenzene (DNFB) H-acid stain for tyrosine histidine and tryptophan, coupled tetrazonium reaction with pre-iodination for histidine, diazotization and Millon's reaction for tyrosine revealed strong staining in the granular precursor of ameloblastic cytoplasma, pre- and young enamel layers. But only alloxan-Schiff stain showed a more intense stainability in the dentin matrix and pre-enamel than the young enamel matrix. The reaction of sulfhydryl and disulfide groups was observed in the matrix-forming ameloblast and Tomes' process with some variation of staining in the enamel matrix. The present histochemical results of protein groups in the enamel matrix were similar to those of the cornified layer of skin.

Ameloblasts moderately stained with DNFB H-acid, coupled tetrazonium and alloxanschiff reaction were similarly stained in both matrix-forming stage and reduced stage. Increasing stainability of odontoblasts was limited in the matrix-forming stage.

Pronounced changes of staining intensity in the enamel matrix for protein groups were found especially in the intra-rod organic substance with progress of mineralization and growth of deposited mineral crystallites, starting from the region of apex of cusp, parallel to the dentino-enamel junction.

The stainability of the dentin matrix for protein groups increases following decalcification, and non-decalcified dentin was weakly stained for the alloxan-schiff method, coupled tetrazonium reaction, sulfhydryl and disulfide method.

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  1. Department of Oral Surgery, Osaka University Dental School, Japan

    Yasuo Deguchi

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  1. Yasuo Deguchi
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Deguchi, Y. Histochemical observations of protein-bound amino groups in human developing deciduous teeth. Histochemie 7, 357–369 (1966). https://doi.org/10.1007/BF00306624

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