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Determining feulgen-DNA of individual chromosomes by fluorescence cytophotometry with incident light

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Summary

A microfluorometric method for measuring the DNA content of chromosomes is described. The measurements were made with the MPV (microscope photometer with adjustable measuring diaphragm, E. Leitz, Wetzlar) with an incident light fluorescence illuminator of 0,05% Feulgen (pararosaniline) stained chromosomes of the cell line B 14 FAF 28 of the Chinese hamster. The variation coefficient of the measurements ranged between 4 and 11%. The measured values correlate well with DNA measurements quoted in the literature for absorption cytophotometry and length measurements for the same chromosomes.

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Authors and Affiliations

  1. Kinderpoliklinik der Universität, München

    Barnim Nitsch, Jan -Diether Murken & Hans -Jürgen Brück

Authors
  1. Barnim Nitsch
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  2. Jan -Diether Murken
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  3. Hans -Jürgen Brück
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Additional information

With support from the Deutsche Forschungsgemeinschaft, AZ Mu 258/3.

Parts of the results reported here are submitted by Barnim Nitsch to the medical faculty of Munich in fulfilment of the requirements for the Dr. med. degree.

We thank Dr. K.-R. Trott, Gesellschaft für Strahlenforschung mbH, Neuherberg, for the original cell cultures of the Chinese hamster; Dr. F. Back, Institut für Hämatologie der GSF, München, for allowing us to use the MPV; Dr. K. Moritz, Zoologisches Institut der Universität München, for carrying out the absorption measurements on the UMSP I.

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Nitsch, B., Murken, J.D. & Brück, H.J. Determining feulgen-DNA of individual chromosomes by fluorescence cytophotometry with incident light. Histochemie 23, 254–265 (1970). https://doi.org/10.1007/BF00306427

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  • DOI: https://doi.org/10.1007/BF00306427

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