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5′-Nucleotidase

II. The significance of 5′-nucleotidase in the metabolism of nucleotides studied by histochemical and biochemical methods

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Summary

To get more insight in the function of 5′-nucleotidase catabolic and anabholic processes were investigated in which 5-nucleotides are involved. The catabolism of adenosine-5-monophosphate was studied by investigating the reaction products obtained after incubation of homogenates of several organs of rat and mouse with adenosine-5-monophosphate and with adenosine. Two experimental tumours of the mouse were investigated in the same way. It was found that in tissues containing a high activity of 5′-nucleotidase other enzymes involved in the catabolism of 5-nucleotides, such as nucleosidase, adenosine deaminase and adenosine-5-monophosphate deaminase could also be demonstrated.

The anabolic processes in which 5-nucleotides are involved had been studied by investigating the incorporation of tritium-labeled thymidine in several tissues of the mouse. It appeared that in cells showing a high 5′-nucleotidase activity no incorporation of radioactive thymidine could be found, while in cells showing incorporation of thymidine enzyme activity could not be demonstrated.

A discussion is given about the possible role of 5′-nucleotidase in the control of nucleic acid biosynthesis and in the catabolism of nucleic acids.

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Abbreviations

DNA:

deoxyribonucleic acid

RNA:

ribonucleic acid

AMP:

Adenosine-5′-monophosphate

ADP:

Adenosine-5′-diphosphate

ATP:

Adenosine-5′-triphosphate

IMP:

Inosine-5′-monophosphate

GMP:

Guanosine-5′-monophosphate

GDP:

Guanosine-5′-diphosphate

GTP:

guanosine-5′-triphosphate

CMP:

Cytidine-5′-monophosphate

CDP:

Cytidine-5′-diphosphate

CTP:

Cytidine-5′-triphosphate

UMP:

Uridine-5′-monophosphate

UDP:

Uridine-5′-diphosphate

UTP:

Uridine-5′-triphosphate

TMP:

Thymidine-5′-monophosphate

TDP:

Thymidine-5′-diphosphate

TTP:

Thymidine-5′-triphosphate

Ado:

Adenosine

Ad:

Adenine

Ino:

Inosine

Hypox:

Hypoxanthine

Xanth:

Xanthine

Xantho:

Xanthosine

Guano:

Guanosine

Gua:

Guanine

Ura:

Uracil

U:

Uridine

Cyt:

Cytidine

Cyto:

Cytosine

Thym:

Thymidine

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Authors and Affiliations

  1. Department of Pathology, Biochemical Section, University of Groningen, Groningen, The Netherlands

    M. J. Hardonk & J. Koudstaal

Authors
  1. M. J. Hardonk
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  2. J. Koudstaal
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Additional information

The corresponding deoxy-compounds have been indicated with the prefix d for instance dCMP, deoxycytidine-5′-monophosphate.

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Hardonk, M.J., Koudstaal, J. 5′-Nucleotidase. Histochemie 12, 18–28 (1968). https://doi.org/10.1007/BF00306344

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  • DOI: https://doi.org/10.1007/BF00306344

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