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On the localization of a prostaglandin-dehydrogenase activity in the kidney

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Summary

Using the kidneys from white rats a method for the localization of a prostaglandin-dehydrogenase activity is presented.

The activity demonstrated showed NAD dependence and displayed a high substrate specificity.

The most pronounced activity was observed in the thick ascending limb of the loop of Henle and in the distale tubule. Lesser activity was found in the collecting tubules of the inner medulla, in the interstitial cells of the medulla, in the epithelial cells of the pelvis, in the tunica media of the cortical arteries and arterioles and in the visceral epithelium of the renal corpuscles.

The pitfalls of the cytochemistry of the co-enzyme linked dehydrogenases are discussed and correlated to the present study. Similarly the observations noticed is discussed in relation to the metabolism and biological activity of the prostaglandins.

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References

  • Andersen, H.: Sulphydryl “Nothing dehydrogenase” activity and lactic dehydrogenase-NADH2 cytochrome c reductase reactions in tissues of the human foetus. Acta histochem. (Jena) 21, 120–134 (1965).

    Google Scholar 

  • Änggård, E.: The biological activities of three metabolites of prostaglandin E1. Acta physiol. scand. 66, 509–510 (1966).

    Google Scholar 

  • —, and S. Bergström: Biological effects of an unsaturated trihydroxy acid (PGF2α) from normal swine lung. Acta physiol. scand. 58, 1–12 (1963).

    Google Scholar 

  • —, K. Green, and B. Samuelsson: Synthesis of tritium-labeled prostaglandin E2 and studies on its metabolism in guinea pig lung. J. biol. Chem. 240, 1932–1940 (1965).

    Google Scholar 

  • —, and B. Samuelsson: Prostaglandins and related factors. Metabolism of prostaglandin E1 in guinea pig lung. The structures of two metabolites. J. biol. Chem. 239, 4097–4102 (1964).

    Google Scholar 

  • —: The metabolism of prostaglandin E3 in guinea pig lung. Biochemistry 4, 1864–1871 (1965).

    Google Scholar 

  • —: Biosynthesis of prostaglandins from arachidonic acid in guinea pig lung. J. biol. Chem. 240, 3518–3521 (1965).

    Google Scholar 

  • —: Purification and properties of a 15-hydroxyprostaglandin dehydrogenase from swine lung. Ark. Kemi 25, 293–300 (1966).

    Google Scholar 

  • —: The metabolism of prostaglandins in lung tissue. In: Prostaglandins. Proc. 2nd Nobel Symp. Stockholm 1966, ed. by S. Bergström and B. Samuelsson, p. 97–105. Stockholm: Almqvist & Wiksell 1967.

    Google Scholar 

  • Bergström, S.: Prostaglandins, a group of hormonal compounds of widespread occurrence. Biochem. Pharmacol. 12, 413–414 (1963).

    Google Scholar 

  • —: Prostaglandins. Recent Progr. Hormone Res. 22, 153–175 (1966).

    Google Scholar 

  • —: Prostaglandins. Members of a new hormonal system. Science 157, 382–391 (1967).

    Google Scholar 

  • —, H. Danielsson, D. Klenberg, and B. Samuelsson: The enzymatic conversion of essential fatty acids into prostaglandins. J. biol. Chem. 239, PC4006-PC4008 (1964).

    Google Scholar 

  • —, and B. Samuelsson: The enzymatic formation of prostaglandin E2 from arachidonic acid. Prostaglandins and related factors 32. Biochim. biophys. Acta (Amst.) 90, 207–210 (1964).

    Google Scholar 

  • — and B. Samuelsson: Prostaglandins. Ann. Rev. Biochem. 34, 101–108 (1965).

    Google Scholar 

  • Brooke, M. H.: The role of substantive tetrazolium compounds and of phenazine methosulfate (PMS) in the false localization of histochemical reactions. J. Histochem. Cytochem. 13, 701 (1965).

    Google Scholar 

  • Conklin, J. L.: A cytochemical study of lipid, glycogen and lactate dehydrogenase in the developing liver. J. exp. Zool. 155, 151–160 (1964).

    Google Scholar 

  • —: Phenazine methosulfate, its use in evaluating activity of dehydrogenase systems of avian liver. Stain Technol. 41, 105–113 (1966).

    Google Scholar 

  • Daniels, E. G., J. W. Hinman, B. A. Johnson, F. P. Kupiecki, J. W. Nelson, and J. E. Pike: The isolation of an additional prostaglandin derivative from the enzymatic cyclization of homo-γ-linolenic acid. Biochem. biophys. Res. Commun. 21, 413–417 (1965).

    Google Scholar 

  • —, B. E. Leach, and E. E. Muirhead: Identification of prostaglandin E2 as the principal vasodepressor lipid of rabbit renal medulla. Nature (Lond.) 215, 1298–1299 (1967).

    Google Scholar 

  • Dariush Fahimi, H., and C. R. Amarasingham: Cytochemical localization of lactic dehydrogenase in white skeletal muscle. J. Cell Biol. 22, 29–48 (1964).

    Google Scholar 

  • Dorp, D. A. van, R. K. Beerthuis, D. H. Nugteren, and H. Vonkeman: The biosynthesis of prostaglandins. Biochim. biophys. Acta (Amst.) 90, 204–207 (1964).

    Google Scholar 

  • —, G. H. Jouvenaz, and C. B. Struijk: The biosynthesis of prostaglandin in pig eye iris. Biochim. biophys. Acta (Amst.) 137, 396–399 (1967).

    Google Scholar 

  • Granström, E., U. Inger, and B. Samuelsson: The structure of an urinary metabolite of prostaglandin F1α in the rat. J. biol. Chem. 240, 457–461 (1965).

    Google Scholar 

  • Hamberg, M., and B. Samuelsson: Isolation and structure of a new prostaglandin from human seminal plasma. Biochim. biophys. Acta (Amst.) 106, 215–217 (1965).

    Google Scholar 

  • —: New groups of naturally occurring prostaglandins. In: Prostaglandins. Proc. 2nd Nobel Symp., Stockholm june 1966, ed. by S. Bergström and B. Samuelsson, p. 63–70. Stockholm: Almqvist & Wiksell 1967.

    Google Scholar 

  • —: Prostaglandins in human seminal plasma. J. biol. Chem. 241, 257–263 (1966).

    Google Scholar 

  • Hashimoto, T., J. S. Kaluza, and M. S. Burstone: The effect of menadione and phenazine methosulfate on the tetrazolium reduction system under histochemical conditions. J. Histochem. Cytochem. 12, 797–804 (1964).

    Google Scholar 

  • Hickler, R. B., D. P. Lauler, C. A. Saravis, and G. W. Thorn: Vasodepressor lipid from the renal medulla. Canad. med. Ass. J. 90, 280–287 (1964).

    Google Scholar 

  • Hitzeman, J. W.: Observations on the subcellular localization of oxidative enzymes with Nitro Blue Tetrazolium. J. Histochem. Cytochem. 11, 62–70 (1963).

    Google Scholar 

  • Horton, E. W., and I. H. M. Main: A comparison of the biological activities of four prostaglandins Brit. J. Pharmacol. 21, 182–189 (1963).

    Google Scholar 

  • Jasmin, G.: Histochimie enzymatique du nephron chez le rat. Acta histochem. (Jena) 26, 160–168 (1967).

    Google Scholar 

  • Kataoka, K., P. W. Ramwell, and S. Jessup: Prostaglandins. Localization in subcellular particles of rat cerebral cortex. Science 157, 1187–1189 (1967).

    Google Scholar 

  • Kriz, W.: Der architektonische und funktionelle Aufbau der Rattenniere. Z. Zellforsch. 82, 495–535 (1967).

    Google Scholar 

  • Lee, J. B.: Chemical and physiological properties of renal prostaglandins. In: Prostaglandins. Proc. 2nd Nobel Symp. Stockholm 1966, ed. by S. Bergström and B. Samuelsson, p. 197–210. Stockholm: Alqvist & Wiksell 1967.

    Google Scholar 

  • —, B. G. Covino, B. H. Takman, and E. R. Smith: Chemical and physiological properties of medullin. Clin. Res. 13, 310 (1965).

    Google Scholar 

  • —: Renomedullary vasodepressor substance, medullin. Isolation, chemical characterization and physiological properties. Circulat. Res. 17, 57–77 (1965).

    Google Scholar 

  • —, K. Crowshaw, and B. H. Takman: Isolation and identification of prostaglandin E2 from rabbit kidney medulla. J. clin. Invest. 46, 1082–1083 (1967).

    Google Scholar 

  • —: J. Z. Gougoutas, B. H. Takman, E. G. Daniels, M. P. Grostic, J. E. Pike, J. W. Hinman, and E. E. Muirhead: Vasodepressor and hypertensive prostaglandins of PGE type with emphasis on the identification of medullin as PGE2-217. J. clin. Invest. 45, 1036 (1966).

    Google Scholar 

  • Lehrer, G. M., D. Silides, and M. F. Farmer: Pyridine nucleotide dependent dehydrogenases: A comparison of quantitative and slide histochemistry. J. Histochem. Cytochem. 13, 700–701 (1965).

    Google Scholar 

  • McMillan, P. J.: Differential demonstration of muscle and heart type lactic dehydrogenase of rat muscle and kidney. J. Histochem. Cytochem. 15, 21–31 (1967).

    Google Scholar 

  • Möllendorff, W. v.: Exkretionsorgane. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. VII/I. Berlin: Springer 1930.

    Google Scholar 

  • Muirhead, E. E., E. Daniels, J. E. Pike, and J. W. Hinman: Renomedullary antihypertensive lipids and prostaglandins. In: Prostaglandins. Proc. 2nd Nobel Symp. Stockholm 1966, ed. by S. Bergström and B. Samuelsson, p. 183–196. Stockholm: Almqvist & Wiksell 1967.

    Google Scholar 

  • Nissen, H. G. M.: On lipid droplets in renal interstitial cells. I. A histochemical study. Z. Zellforsch. 83, 76–81 (1967).

    Google Scholar 

  • —: On lipid droplets in renal interstitial cells. II. A histological study on the number of droplets in saltdepletion and acute saltrepletion. Z. Zellforsch. 85, 483–491 (1968).

    Google Scholar 

  • Novikoff, A. B., W. -Y. Shin, and J. Drucker: Mitochondrial localization of oxidative enzymes: Staining results with two tetrazolium salts. J. biophys. biochem. Cytol. 9, 47–61 (1961).

    Google Scholar 

  • Nugteren, D. H., R. K. Beerthuis, and D. A. van Dorp: The enzymic conversion of allcis 8, 11, 14-eicosatrienoicacid into prostaglandin E1. Rec. Trav. chim. Pays-Bas 85, 405–419 (1966).

    Google Scholar 

  • Orloff, J., and J. Grantham: The effect of prostaglandin (PGE1) on the permeability response of rabbit collecting tubules to vasopressin. In: Prostaglandins. Proc. 2nd Nobel Symp. Stockholm 1966, ed. by S. Bergström and B. Samuelsson, p. 143–146. Stockholm: Almqvist & Wiksell 1967.

    Google Scholar 

  • —, J. S. Handler, and S. Bergström: The effect of prostaglandin (PGE1) on the permeability response of toad bladder to vasopressin, theophylline and adenosine-3,5-monophosphat. Nature (Lond.) 205, 397–398 (1965).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, theoretical and applied, 2nd ed. London: J. & A. Churchill 1960.

    Google Scholar 

  • —, and R. Hess: Substantivity and other factors responsible for formazan patterns in dehydrogenase histochemistry. Experientia (Basel) 17, 136–141 (1961).

    Google Scholar 

  • Pette, D., and H. Brandau: Enzyme-Histiogramme und Enzymaktivitätsmuster der Rattenleber. Enzym, biol. clin. 6, 79–122 (1966).

    Google Scholar 

  • Pickles, V. R.: Prostaglandins. Biol. Rev. 42, 614–652 (1967).

    Google Scholar 

  • Samuelsson, B.: Synthesis of tritium-labeled prostaglandin E1 and studies on its distribution and excretion. J. biol. Chem. 239, 4091–4096 (1964).

    Google Scholar 

  • Shaw, C. R., and A. L. Koen: On the identity of “Nothing Dehydrogenase”. J. Histochem. Cytochem. 13, 431–433 (1965).

    Google Scholar 

  • Strong, C. G., and D. F. Bohr: Effects of prostaglandins E1, E2, A1 and F1 on isolated vascular smooth muscle. Amer. J. Physiol. 213, 725–733 (1967).

    Google Scholar 

  • —, R. Boucher, W. Nowaczynsky, and J. Genest: Renal vasodepressor lipid. Proc. Mayo Clin. 41, 433–452 (1966).

    Google Scholar 

  • Thomas, E., and A. G. E. Pearse: The fine localization of dehydrogenases in the nervous system. Histochemie 2, 266–282 (1961).

    Google Scholar 

  • Walker, D. G., and A. M. Seligman: The use of formalin fixation in the cytochemical demonstration of succinic and DPN and TPN-dependent dehydrogenases in mitochondria. J. Cell Biol. 16, 465–469 (1963).

    Google Scholar 

  • Wallach, D. P.: The enzymic conversion of aracidonic acid to prostaglandin E2 with acetone powder preparations of bovine seminal vesicles. Life Sci. 4, 361–364 (1965).

    Google Scholar 

  • Williams, D., and H. J. Whiteley: Variations in formazan production in mitochondrial dehydrogenase studies on osmotically protected fresh frozen sections. J. Histochem. Cytochem. 11, 89–95 (1963).

    Google Scholar 

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Authors and Affiliations

  1. Anatomy Department C, University of Copenhagen, Copenhagen Ø, Denmark

    H. M. issen

  2. Anatomy Department A, University of Copenhagen, Laboratory of Cyto and Histochemistry, Copenhagen Ø, Denmark

    H. Andersen

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  1. H. M. issen
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  2. H. Andersen
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Additional information

This work was supported by a grant from the Danish State Research Foundation.

The authors thank Dr. John E. Pike of the Upjohn Company, Kalamazoo, Michigan, for the gift of prostaglandins used in these studies.

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issen, H.M., Andersen, H. On the localization of a prostaglandin-dehydrogenase activity in the kidney. Histochemie 14, 189–200 (1968). https://doi.org/10.1007/BF00306340

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