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Kontrollierte Gefriertrocknung von Kryostatschnitten

Controlled freeze-drying of cryostat sections

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Summary

In contrast to freeze-drying of tissue blocks the freeze-drying of cryostat sections can be controlled manometrically. The Pirani-element has been found to be fully sufficient for this task. It is therefore possible to shorten the procedure without any risk by regulating the cooling temperature. The pressure curve shows clearly, when the sections have reached optimum humidity for histological and histochemical staining.

Zusammenfassung

Die Gefriertrocknung von Kryostatschnitten kann, im Gegensatz zur Trockung von Gewebsblöcken, manometrisch kontrolliert werden. Das in die herkömmlichen Gefriertrockungsanlagen eingebaute Pirani-Element genügt für diesen Zweck vollauf. Es ist damit möglich, das Verfahren ohne jedes Risiko durch Regelung der Kühltemperatur abzukürzen. Die Druckkurve zeigt eindeutig, wann die Schnitte eine für histologische und histochemische Reaktionen optimale Restfeuchtigkeit erreicht haben.

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  1. Anatomisches Institut der Universität Würzburg, Bundesrepublik Deutschland

    J. Winckler

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  1. J. Winckler
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Winckler, J. Kontrollierte Gefriertrocknung von Kryostatschnitten. Histochemie 22, 234–240 (1970). https://doi.org/10.1007/BF00306100

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  • DOI: https://doi.org/10.1007/BF00306100

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