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Specificity of the glutaraldehyde silver technique for catecholamines and related compounds

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Summary

Studies regarding the specificity of glutaraldehyde-silver technique showed that this technique, used as described previously is positive for noradrenalin, dopamine, melanin and lipofuscin. Adrenalin and 5-hydroxytryptamin storing cell produce negative results.

Light microscopy does not reveal 5-hydroxytryptamin containing cells even following prolonged (30 minutes) silver treatment, but a fine silver precipitate appears under these conditions in 5-hydroxytryptamin containing granules when the material is observed under the electron microscopy.

Dopamine is not revealed by light microscopy because the silver precipitate is very fine and scattered in the granule but it is easily seen by electron microscopy.

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Abbreviations

DA:

dopamine

NA:

noradrenalin

A:

adrenalin

5-HT:

5-hydroxytrdytamin

GA:

glutaraldehyde

S:

silver

References

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Authors and Affiliations

  1. Instituto de Biología y Medicina Experimental, Obligado, 2490, Buenos Aires, Argentina

    Miguel A. Cannata, Sara R. Chiocchio & Juan H. Tramezzani

Authors
  1. Miguel A. Cannata
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  2. Sara R. Chiocchio
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  3. Juan H. Tramezzani
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Cannata, M.A., Chiocchio, S.R. & Tramezzani, J.H. Specificity of the glutaraldehyde silver technique for catecholamines and related compounds. Histochemie 12, 253–264 (1968). https://doi.org/10.1007/BF00306003

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  • DOI: https://doi.org/10.1007/BF00306003

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