Skip to main content
SpringerLink
Log in

Studies on the histochemical “leucine aminopeptidase” reaction

V. Cathepsin B as a potential effector of LNA hydrolysis

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

Cathepsin B has been purified by gel filtration. A complete separation from cathepsin C and D was achieved. This particular proteinase hydrolysed the chromogenic substrates BANA and LNA. Cathepsin B must thus be included among the potential initiators of a positive histochemical LNase reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Haba, G. de la, P. S. Cammarata, and S. N. Timasheff: The partial purification and some physical properties of cathepsin C from beef spleen. J. biol. Chem. 234, 316–319 (1959).

    Google Scholar 

  • Smith, E. L., and D. H. Spackman: Leucine aminopeptidase. V. Activation, specificity, and mechanism of action. J. biol. Chem. 212, 271–299 (1955).

    Google Scholar 

  • Snellman, O., and B. Sylvén: Haptoglobin acting as a natural inhibitor of cathepsin B activity. Nature (Lond.) 216, 1033 (1967).

    Google Scholar 

  • Sylvén, B., and I. Bois: Studies on the histochemical “leucine aminopeptidase” reaction. I. Identity of the enzymes possibly involved. Histochemie 3, 65–78 (1962).

    Google Scholar 

  • , and I. Bois-Svensson: Studies on the histochemical “leucine aminopeptidase” reaction. IV. Chemical and histochemical characterization of the intracellular and stromal LNA reactions in solid tumor transplants. Histochemie 4, 135–149 (1964).

    Google Scholar 

  • and O. Snellman: A new metal-dependent enzyme present in cathepsin C preparations. Biochim. biophys. Acta (Amst.) 65, 350–353 (1962).

    Google Scholar 

  • : Studies on the histochemical “leucine aminopeptidase” reaction. III. On the different LNA-splitting enzymes from spleen. Histochemie 3, 484–486 (1964).

    Google Scholar 

  • Vanha-Perttula, T. P. J., and V. K. Hopsu: The ovarial enzymes hydrolysing L-leucyl and DL-alanyl-β-naphthylamide. Histochemie 6, 34–45 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Cancer Research Division of Radiumhemmet, Karolinska Institute, Stockholm 60, Sweden

    B. Sylvén & O. Snellman

Authors
  1. B. Sylvén
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. Snellman
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by grants from the Jubilee Fund, Cancer Society of Stockholm and the Swedish Natural Research Council.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sylvén, B., Snellman, O. Studies on the histochemical “leucine aminopeptidase” reaction. Histochemie 12, 240–243 (1968). https://doi.org/10.1007/BF00306001

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00306001

Keywords

Search

Navigation