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Enzym-Topochemie von Frühentwicklung und Implantation des Kaninchens

I. Glykogenstoffwechsel

Enzyme topochemistry of the early development and implantation in the rabbit

I. Metabolism of glycogen

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Summary

The early stages of development from the ovulation to implantation (including the Fallopian tube and uterus) are studied in the rabbit. The distribution of enzymes of the metabolism of glycogen is demonstrated.

For phosphorylase, a new method is used. A high activity is found in the epithelium of the Fallopian tube and in the egg during cleavage. In blastocyst formation, the degradation of glycogen seems to be important, whereas phosphorylase activity in the epithelium of the uterus at the time of implantation is correlated with a storage of glycogen.

Glycogen synthetase could not be demonstrated in these tissues.

The possibility of a physiologic role of amylase is discussed.

Zusammenfassung

An Frühstadien der Entwicklung des Kaninchens von der Ovulation bis zur Implantation (einschließlich der umgebenden mütterlichen Gewebe) wird die Topochemie von Enzymen des Glykogenstoffwechsels untersucht.

Für den Phosphorylase-Nachweis kommt eine neue methodische Variante zur Anwendung. Furchungsstadien sind gekennzeichnet durch eine hohe Aktivität in Keim und Tubenepithel. Beim Übergang in das Blastozytenstadium scheint der Glykogenabbau von besonderer Bedeutung zu sein. Gegen den Zeitpunkt der Implantation beginnt, gemessen an Phosphorylaseaktivität und Glykogenkonzentration, erneut ein reger Glykogenstoffwechsel, jetzt im Uterusepithel.

Glykogensynthetase war in den hier interessierenden Strukturen nicht nachweisbar.

Eine eventuelle Bedeutung von Amylase wird diskutiert (Tubenepithel, Blastozystenhöhle).

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Abbreviations

EDTA:

Äthylendiamintetraazeta

PAS:

Perjodsäure-Schiff-Reaktion

p.c:

post coitum

h p.c., d p.c.:

Stunden p.c., Tage p.c.

PVP:

Polyvinylpyrrolidon

UDP:

Uridindiphosphat

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Authors and Affiliations

  1. Anatomisches Institut der Universität Marburg a. d. Lahn, Deutschland

    H. W. Denker

  2. Max-Planck-Institut für Immunbiologie, Freiburg i. Br. Stefan-Meier-Str. 8, BRD-7800, Deutschland

    H. W. Denker

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Denker, H.W. Enzym-Topochemie von Frühentwicklung und Implantation des Kaninchens. Histochemie 25, 256–267 (1971). https://doi.org/10.1007/BF00305944

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