Skip to main content
SpringerLink
Log in

Histochemical demonstration of rat liver glycogen phosphorylase activity with iron (Fe++)

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

A new histochemical method for light microscopic demonstration of liver glycogen phosphorylase activity has been introduced in this study.

The method demonstrates phosphorylase activity by precipitating phosphate ions, liberated in the reaction catalyzed by the enzyme, with Fe++ present in the incubating medium. The precipitate is visualized as ferrous sulphide.

The new glycogen, formed in the same reaction, can also be demonstrated in this method after staining with iodine.

The lobular localization of the reaction products obtained according to this method in the liver, corresponds well to that obtained according to earlier methods for the demonstration of only new-formed glycogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Belfanti, S., Contardi, A., Ercoli, A.: Inactivation and reactivation of the phosphatases of animal organs. Biochem. J. 29, 842–846 (1935)

    Google Scholar 

  • Cleland, W. W.: Dithiothreitol, a new protective reagent for SH groups. Biochemistry 3, 480–482 (1964)

    Google Scholar 

  • Coimbra, A., Leblond, C. P.: Sites of glycogen synthesis in rat liver cells as shown by electron microscope radioautography after administration of glucose-H3. J. Cell. Biol. 30, 151–175 (1966)

    Google Scholar 

  • Cori, C. F., Schmidt, G., Cori, G. T.: The synthesis of a polysaccharide from glucose-1-phosphate in muscle extract. Science (N. Y.) 89, 464–465 (1939)

    Google Scholar 

  • Dorn, A.: Die Hemmwirkung des 2,4-Dichlorophenoxyazetats auf den histochemischen Nachweis der Phosphorylase mit Hilfe der Bleifällungstechnik. Act histochem. (Jena) 30, 391–392 (1968)

    Google Scholar 

  • Dunnick, J. K., Marinetti, G. V., Greenland, P.: Effect of detergents and sodium fluoride on the enzyme activities of the rat liver plasma membrane. Biochem. biophys. Acta (Amst.) 266, 684–694 (1972)

    Google Scholar 

  • Eränkö, O., Palkama, A.: Improved localization of phosphorylase by the use of polyvinyl pyrrolidone and high substrate concentration. J. Histochem. Cytochem. 9, 585 (1961)

    Google Scholar 

  • Goodlad, G. A. J., Mills, G. T.: The acid phosphatases of rat liver. Biochem. J. 66, 346–354 (1957a)

    Google Scholar 

  • Goodlad, G. A. J., Mills, G. T.: The hydrolysis of glucose-1-phospate by rat-liver extracts. Biochem. J. 66, 354–357 (1957b)

    Google Scholar 

  • Hers, H. G., De Wulf, H., Stalmans, W.: The control of glycogen metabolism in the liver. FEBS Letters 12, 73–82 (1970)

    Google Scholar 

  • Hori, S. H.: Cytological phosphorylase locations in rat liver and muscle as shown by a lead precipitation method. Stain Technol. 39, 275–278 (1964)

    Google Scholar 

  • Hori, S. H.: Fine-structure locations of α-glucan phosphorylase as shown by lead precipitation and electron microscopy. Stain Technol. 41, 91–95 (1966)

    Google Scholar 

  • Illingworth, B., Cori, G. T.: Crystalline muscle phosphorylase. In: Biochemical Preparations, ed. H. E. Carter, vol. 3, p. 1–9. New York: John Wiley & Sons, Inc. 1953

    Google Scholar 

  • Kurata, Y., Maeda, S.: Histochemical demonstration of pyrophosphatase. Stain Technol. 31, 13–16 (1956)

    Google Scholar 

  • Lindberg, L.-A.: Lead and some other metals in the histochemical demonstration of rat liver glycogen phosphorylase activity. Histochemie 36, 347–353 (1973)

    Google Scholar 

  • Lindberg. L.-A., Palkama, A.: Effect of some chemical factors on the histochemical demonstration of liver glycogen phosphorylase activity. J. Histochem. Cytochem. 20, 331–335 (1972)

    Google Scholar 

  • Najjar, V. A.: The isolation and properties of phosphoglucomutase. J. biol. Chem. 175, 281–290 (1948)

    Google Scholar 

  • Passonneau, J. V., Gatfield, P. D., Schulz, D. W., Lowry, O. H.: An enzymic method for measurement of glycogen. Analyt. Biochem. 19, 315–326 (1967)

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, Theoretical and applied, 3rd ed., vol. II. London: J. & A. Churchill 1972

    Google Scholar 

  • Revel, J. P., Napolitano, L., Fawcett, D. W.: Identification of glycogen in electron micrographs of thin tissue sections. J. biophys. biochem. Cytol. 8, 575–589 (1960)

    Google Scholar 

  • Ryman, B. E., Whelan, W. J.: New aspects of glycogen metabolism. In: Advances in enzymology, ed. F. F. Nord, vol. 34, p. 285–443. New York; John Wiley & Sons, Inc. 1971

    Google Scholar 

  • Sutherland, E. W.: The effect of the hyperglycemic factor and epinephrine on enzyme systems of liver and muscle. Ann. N. Y. Acad. Sci. 54, 693–706 (1951)

    Google Scholar 

  • Takeuchi, T., Glenner, G. G.: The histochemical demonstration of UDPG-pyrophosphorylase (uridyl transferase) activity. J. Histochem. Cytochem. 9, 623–624 (1961)

    Google Scholar 

  • Takeuchi, T., Kuriaki, H.: Histochemical detection of phosphorylase in animal tissues. J. Histochem. Cytochem. 3, 153–160 (1955)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy and Embryology, College of Veterinary Medicine, Helsinki

    L. -A. Lindberg

Authors
  1. L. -A. Lindberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lindberg, L.A. Histochemical demonstration of rat liver glycogen phosphorylase activity with iron (Fe++). Histochemie 36, 355–365 (1973). https://doi.org/10.1007/BF00305714

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00305714

Keywords

Search

Navigation