Skip to main content
SpringerLink
Log in

Artunspezifische und reversible Wachstumshemmung durch Chalone in Plattenepithelkarzinomen des Menschen in vitro

Species-non-specific and reversible growth inhibition by chalones in human epidermoid carcinomas in vitro

  • Originalarbeiten
  • Published:
Zeitschrift für Krebsforschung und Klinische Onkologie Aims and scope Submit manuscript

Summary

Human epidermoid carcinomas and adenocarcinomas from the respiratory tract were established as cell cultures and grown on Cell finder film slides (Microlab, Holland).

The cultures showed lack of contact inhibition, and cytogenetical analyses revealed both numerical and structural karyotype anomalies, with various abnormal chromosome stemlines.

Pig and mouse skin extracts supposed to contain the epidermal G2 chalone and mouse liver extracts prepared in a similar way were added to the cell cultures, which were then assayed for mitotic inhibition by means of the Colcemid technique, i.e. the number of Colcemid-arrested mitoses per 1000 cells during 4 h was counted.

The results indicate that the species-non-specificity and the reversibility of the epidermal G2 chalone activity, previously demonstrated in both malignant and non-malignant animal tissues and in human epidermis, is to be found even in human epidermoid carcinomas in vitro.

The reversibility of the chalone-induced mitotic depression could be demonstrated by repeated counts of the number of proliferating cells in the cultures before and after addition of chalone extracts.

Whether this holds true for malignant human tumours in general remains, however, to be seen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Bullough,W.S.: The control of mitotic activity in adult mammalian tissues. Biol. Rev. 37, 307–342 (1962)

    Google Scholar 

  • Bullough,W.S.: The Evolution of Differentiation. London—New York: Academic Press 1967

    Google Scholar 

  • Bullough,W.S., Laurence,E.B.: Mitotic control by internal secretion: The role of the chalone-adrenalin complex. Exp. Cell. Res. 33, 176–194 (1964)

    Google Scholar 

  • Bullough,W.S., Laurence,E.B.: Chalones and cancer. Nature (Lond.) 220, 134–135 (1963)

    Google Scholar 

  • Chopra,D.P., Flaxman,B.A.: Mitotic inhibition of epidermal cells from psoriasis lesions in vitro by extracts from normal human skin. J. Nat. Cancer Inst. 50, 281–283 (1973)

    Google Scholar 

  • Chopra,D.P., Yu,R.J., Flaxman,B.A.: Demonstration of a tissue specific inhibitor of mitosis of human epidermal cells in vitro. J. Invest. Dermatol. 59, 207–210 (1972)

    Google Scholar 

  • Elgjo,K.: The stability of the epidermal mitosis inhibiting factor (chalone) in water solution. Acta Path. Microbiol. Scand. 76, 31–34 (1969)

    Google Scholar 

  • Elgjo,K.: Epidermal growth inhibitors (chalones) in dermis and serum. Virchows Arch. Abt. B Zell-path. 13, 14–23 (1973)

    Google Scholar 

  • Elgjo,K., Hennings,H.: Epidermal chalone and cell proliferation in a transplantable squamous cell carcinoma in hamsters. I. In vivo results. Virchows Arch. Abt. B. Zellpath. 7, 1–7 (1971)

    Google Scholar 

  • Forscher,B.K., Houck,J.C. (Editors): Chalones: Concepts and Current Researches. Nat. Cancer Inst., Monogr. 38, 1–233 (1973)

  • Hondius Boldingh,W., Laurence,E.: Extraction, purification and preliminary characterization of the epidermal chalone: A tissue specific mitotic inhibitor obtained from vertebrate skin. Europ. J. Biochem. 5, 191–198 (1968)

    Google Scholar 

  • Houck,J.C. (Editor): Chalones. New York-Amsterdam: North—Holland Publishing Company 1976

    Google Scholar 

  • Iversen,O.H.: Effect of epidermal chalone on human epidermal mitotic activity in vitro. Nature (Lond.) 219, 75 (1968)

    Google Scholar 

  • Iversen,O.H.: Some theoretical considerations on chalones and the treatment of cancer: A review. Cancer Res. 30, 1481–1484 (1970)

    Google Scholar 

  • Iversen,O.H.: The chalones. Acta Pat Microbiol. Scand. Sect A, Suppl 236, 71–76 (1973)

    Google Scholar 

  • Iversen,O.H., Aandahl,E., Elgjo,K.: The effect of an epidermis-specific mitotic inhibitor (chalone) extracted from epidermal cells. Acta Path. Microbiol. Scand. 64, 506–510 (1965)

    Google Scholar 

  • Iversen,O.H., Bjerknes,L: Kinetics of epidermal reaction to carcinogens. Acta Path. Microbiol. Scand. Suppl 165, 1–74 (1963)

    Google Scholar 

  • Korsgaard,R., Burton,D., Isaksson-Forsén,G.: Chalone-induced cell growth inhibition of human epidermoid carcinomas in vitro. I.R.C.S. Med. Sci. 4, 218 (1976)

    Google Scholar 

  • Laurence,E.B., Elgjo,K.: Epidermal chalone and cell proliferation in a transplantable squamous cell carcinoma in hamsters. II. In vitro results. Virchows Arch. Abt. B Zellpath. 7, 8–15 (1971)

    Google Scholar 

  • Marks,F., Grimm,W., Krieg,L.: Disturbance by tumor promotors of epidermal growth control (chalone mechanism). Hoppe-Seyler's Z. Physiol. Chem. 35, 1970–1972 (1972)

    Google Scholar 

  • Mohr,U., Althoff,J., Kinzel,V., Güss,L., Volm,M.: Melanoma regression induced by “chalone”: A new tumor inhibiting principle acting in vivo. Nature (Lond.) 220, 138–139 (1968)

    Google Scholar 

  • Rytömaa,T., Kiviniemi,K.: Regression of generalized leukemia in rat induced by the granulocytic chalone. Eur. J. Cancer 6, 401–410 (1970)

    Google Scholar 

  • Starling,E.H.: Die chemische Koordination der Körpertätigkeiten. Verh. Ges. Naturforsch. Ärzte (Stuttgart) pt. 1, 246–260 (1906)

  • Summary of Proceedings of the Seventeenth International Medical Congress, 6–12 August 1913, Section of Physiology, Br. Med. J. Vol. III, p. 380 (1913)

Download references

Author information

Authors and Affiliations

  1. Institut für Tumorzytogenetik im Wallenberglaboratorium der Universität Lund, S-220 07, Lund, Schweden

    R. Korsgaard

  2. Forschungsinstitut 1 und Lungenklinik des Universitätskrankenhauses Lund, S-221 85, Lund, Schweden

    R. Korsgaard, O. H. Iversen, D. R. Burton & G. Isaksson-Forsén

  3. Institut für Physikalische Chemie 2 im Chemischen Zentrum der Universität Lund, S-220 07, Lund, Schweden

    R. Korsgaard, O. H. Iversen, D. R. Burton & G. Isaksson-Forsén

  4. Institut für Pathologie der Universität Oslo, Oslo 1, Norwegen

    R. Korsgaard, O. H. Iversen, D. R. Burton & G. Isaksson-Forsén

Authors
  1. R. Korsgaard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. H. Iversen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. R. Burton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Isaksson-Forsén
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Wir danken Herrn Dr. W. Hondius Boldingh, Biochemische Forschungsabteilung der N. V.Organon, Oss, Holland, für die freundliche Überlassung des Schweinehaut-Chalonpulvers sowie der Schwedischen Nationalvereinigung gegen Herz- und Lungenkrankheiten und der John und Augusta Persson Stiftung für Medizinische Forschung für die finanzielle Unterstützung.

Rolf Korsgaard ist Empfänger eines persönlichen Forschungsstipendium (746-B74-02P) der Schwedischen Krebsgesellschaft.

Dennis R. Burton ist Empfänger eines CIBA-GEIGY Forschungsstipendium.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Korsgaard, R., Iversen, O.H., Burton, D.R. et al. Artunspezifische und reversible Wachstumshemmung durch Chalone in Plattenepithelkarzinomen des Menschen in vitro. Z. Krebsforsch. 88, 217–221 (1977). https://doi.org/10.1007/BF00305359

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00305359

Search

Navigation