Summary
The main and accessory olfactory bulbs (MOB and AOB) of the rat were immunohistochemically stained with a monoclonal antibody against choline acetyltransferase (ChAT) in order to know the difference in the distribution patterns of cholinergic fibers between these two structures. A few ChAT-immunoreactive cell bodies were found in the superficial and middle parts of the external plexiform layer (EPL) of the MOB, in the granule cell layer (GCL) of the MOB, and in the GCL of the AOB. The frequency in appearance of these cells was 0.9 cells/section in the MOB and 0.3 cells/section in the AOB. While the glomerular layer (GL) and the superficial part of the EPL were most densely innervated in the MOB, the internal plexiform layer received the richest innervation in the AOB. There were no immunoreactive structures in the olfactory nerve layer of the MOB and in the vomeronasal nerve layer and glomerular layer of the AOB. In addition to a relatively homogenous distribution of cholinergic fibers in the MOB and AOB, there were several foci of very dense network of immunoreactive fibers at the posterior level of the OB. These foci formed a part of the modified glomerular complex that was recently identified using 2-deoxyglucose method and was presumed to be related to suckling behaviour in the neonatal rat.
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References
Cajal RyS (1955) The structure of the accessory olfactory lobule. In: Studies on the cerebral cortex (Limbic structures). Translated from the Spanish by Kraft LM. Lloyd-Luke, London
Carson KA, Burd GD (1980) Localization of acetylcholinesterase in the main and accessory olfactory bulbs of the mouse by light and electron microscopic histochemistry. J Comp Neurol 191:353–371
Chao L-P, Karen Kan K-S, Hong F-M (1982) Immunohistochemical localization of choline acetyltransferase in rabbit forebrain. Brain Res 235:65–82
Cuello AC, Sofroniew MV (1984) The anatomy of the CNS cholinergic neurons. Trends Neurosci 7:74–78
Eckenstein F, Sofroniew MV (1983) Identification of central cholinergic neurons containing both choline acetyltransferase and acetylcholinesterase and of central neurons containing only acetylcholinesterase. J Neurosci 3:2286–2291
Funnum F (1973) Recent developments in biochemical investigations of cholinergic transmission. Brain Res 62:497–507
Greer CA, Stewart WB, Teicher MH, Shepherd GM (1982) Functional development of the olfactory bulb and a unique glomerular complex in the neonatal rat. J Neurosci 2:1744–1759
Godfrey DA, Ross CD, Herrmann AD, Tatschinsky FM (1980) Distribution and derivation of cholinergic elements in the rat olfactory bulb. Neurosci 5:273–292
Ichikawa T, Hirata Y (1986) Organization of choline acetyltransferase-containing structures in the forebain of the rat. J Neurosci 6:281–292
Kimura H, McGeer PL, Peng JH, McGeer EG (1981) The central cholinergic system studied by choline acetyltransferase immunohistochemistry in the cat. J Comp Neurol 200:151–201
Macrides F, Davis BJ (1983) The olfactory bulb. In: Emson PC (ed) Chemical neuroanatomy. Raven Press, New York
Ojima H, Yamasaki T, Kojima H, Akashi A (1988) Immunohistochemical conditions for staining choline acetyltransferase (ChAT)-containing axon terminals in the rat. Stain Tech (in press)
de Olmos J, Hardy H, Heimer L (1978) The afferent connections of the main and accessory olfactory bulb formations in the rat: An experimental HRP-study. J Comp Neurol 181:213–244
Pederson PE, Jastreboff PJ, Stewart WB, Shepherd GM (1986) Mapping of an olfactory receptor population that projects to a specific region in the rat olfactory bulb. J Comp Neurol 250:93–108
Pinching AJ, Powell TSP (1971) The neuropile of the glomeruli of the olfactory bulb. J Cell Sci 9:347–377
Rao ZR, Yamano M, Wanaka A, Tatehata T, Shiosaka S, Tohyama M (1987) Distribution of cholinergic neurons and fibers in the hypothalamus of the rat using choline acetyltransferase as a marker. Neurosci 20:923–934
Ross CD, Godfrey DA, Williams AD, Matshinsky FM (1978) Evidence for a cetral origin of cholinergic structures in the olfactory bulb. Neurosci [Abstr] 4:91
Rotter A (1984) Cholinergic receptors. In: Bjorklund A, Hokfelt T, Kuhaf MJ (eds) Handbook of chemical neuroanatomy. Elsevier, Amsterdam New York Oxford
Satoh K, Armstrong DM, Fibiger HC (1983) A comparison of the distribution of central cholinergic neurons as demonstrated by acetylcholinesterase pharmacohistochemistry and choline acetyltransferase immunohistochemistry. Brain Res Bull 11:693–720
Sharma NN (1968) Studies on the histochemical distribution of simple esterase and cholinesterase in the olfactory bulb of the rat. Acta Anat 69:168–175
Shute CCD, Lewis PR (1967) The ascending cholinergic reticular system: Neocortical, olfactory, and subcortical projections. Brain 90:497–520
Sofroniew MV, Campbell PE, Cuello AC, Eckenstein F (1985) Central cholinergic neurons visualized by immunohistochemical detection of choline acetyltransferase. In: Paxinos G (ed) The rat nervous system, Forebrain and Midbrain, vol 1. Academic Press, Sydney Orlando San Diego New York London Toronto Montreal Tokyo
Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley. New York
Teicher MH, Stewart WD, Kauer JS Shepherd GM (1980) Suckling pheromone stimulation of a modified glomerular region in the developing rat olfactory bulb revealed by the 2-deoxyglucose method. Brain Res 194:530–535
Zaborszky L, Carlsen J, Brashear HR, Heimer L (1986) Cholinergic and GABAergic afferents to the olfactory bulb in the rat with special emphasis on the projection neurons in the nucleus of the horizontal limb of the diagonal band. J Comp Neurol 243:488–509
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Ojima, H., Yamasaki, T., Kojima, H. et al. Cholinergic innervation of the main and the accessory olfactory bulbs of the rat as revealed by a monoclonal antibody against choline acetyltransferase. Anat Embryol 178, 481–488 (1988). https://doi.org/10.1007/BF00305035
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DOI: https://doi.org/10.1007/BF00305035