Summary
A method is described which combines the histochemical fluorescence technique of Falck and Hillarp with isotope measurements in the same pieces of tissue. Tissue pieces incubated in isotope solutions were treated for fluorescence microscopy and examined. They were then removed from the microscopical slides, and the radioactivity determined. It was shown that NA1 content and estimated fluorescence intensity were well correlated. The procedure devised is of special value when isotope measurements are needed of structures which can be safely identified only in the fluorescence microscope, and it has been used for quantitative estimations of adrenergic innervation.
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Abbreviations
- Na:
-
noradrenaline
- cpm:
-
counts per minute
- dpm:
-
desintegrations per minute
References
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Olson, L., Hamberger, B., Jonsson, G. et al. Combined fluorescence histochemistry and 3h-noradrenaline measurements of adrenergic nerves. Histochemie 15, 38–45 (1968). https://doi.org/10.1007/BF00304572
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DOI: https://doi.org/10.1007/BF00304572