Summary
Human testes contain trace amounts of heat-stable placental-like alkaline phosphatase. Using a recently described allotype-specific monoclonal antibody (F11) toward placental alkaline phosphatase (PLAP), we show that the frequencies of reactivity of the testis enzymes differ greatly from those of the placental phenotypes. By means of the enzyme inhibitors L-Phe, L-Phe-gly-gly, L-Leu, and L-Leu-gly-gly, the testis enzyme can be clearly distinguished in all cases from the placental enzyme. These results argue that the testis enzyme is not a product of the placental gene and suggest the possible existence of a new locus of alkaline phosphatase.
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References
Beckman G, Beckman L (1975) The placental alkaline phosphatase variant D and natural selection. Relationship to complications of pregnancy and to “the Regan isoenzyme”. Hereditas 81:85–88
Chang CH, Angellis D, Fishman WH (1980) Presence of the rare D-variant heat-stable, placental-type alkaline phosphatase in normal human testis. Cancer Res 40:1506–1510
Doellgast GJ, Fishman WH (1976) L-leucine, a specific inhibitor of a rare human placental alkaline phosphatase phenotype. Nature 259:49–51
Doellgast GJ, Fishman WH (1977) Inhibition of human placental alkaline phosphatase variants by peptides containing L-leucine. Clin Chim Acta 75:449–454
Donald LJ, Robson EB (1974) Rare variants of placental alkaline phosphatase. Ann Hum Genet 37:303–313
Inglis NR, Kirley S, Stolbach LL, Fishman WH (1973) Phenotypes of the Regan isoenzyme and identity between the placental D-variant and the Nagao isoenzyme. Cancer Res 33:1657–1661
Millán JL, Stigbrand T (1981) “Sandwich” enzyme immunoassay for placental alkaline phosphatase. Clin Chem 27:2014–2018
Millán JL, Beckman G, Jeppsson A, Stigbrand T (1982a) Genetic variants of placental alkaline phosphatase as detected by a monoclonal antibody. Hum Genet 60:145–149
Millán JL, Stigbrand T, Ruoslahti E, Fishman WH (1982b) Characterization and use of an allotype-specific monoclonal antibody to placental alkaline phosphatase in the study of cancer-related phosphatase polymorphism. Cancer Res 42:2444–2449
Mullivor RA, Plotkin LI, Harris H (1978) Differential inhibition of the products of the human alkaline phosphatase loci. Ann Hum Genet 42:1–13
Nakayama T, Yoshida M, Kitamura M (1970) L-leucine-sensitive heat stable alkaline phosphatase isoenzyme detected in a patient with pleuritis carcinomatosa. Clin Chim Acta 30:546–548
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Millán, J.L., Eriksson, A. & Stigbrand, T. A possible new locus of alkaline phosphatase expressed in human testis. Hum Genet 62, 293–295 (1982). https://doi.org/10.1007/BF00304541
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DOI: https://doi.org/10.1007/BF00304541